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DNA ends refer to the properties of the ends of linear DNA molecules, which in molecular biology are described as "sticky" or "blunt" based on the shape of the complementary strands at the terminus. In sticky ends, one strand is longer than the other (typically by at least a few nucleotides), such that the longer strand has bases which are left unpaired. In blunt ends, both strands are of equal length – i.e. they end at the same base position, leaving no unpaired bases on either strand.
The concept is used in molecular biology, in cloning, or when subcloning insert DNA into vector DNA. Such ends may be generated by restriction enzymes that break the molecule's phosphodiester backbone at specific locations, which themselves belong to a larger class of enzymes called exonucleases and endonucleases. A restriction enzyme that cuts the backbones of both strands at non-adjacent locations leaves a staggered cut, generating two overlapping sticky ends, while an enzyme that makes a straight cut (at locations directly across from each other on both strands) generates two blunt ends.[1]
^Sullivan, Mary (17 May 2016). Ball. Houghton Mifflin Harcourt Publishing Company. ISBN 9780544819016. OCLC 949423125.
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DNA ends refer to the properties of the ends of linear DNA molecules, which in molecular biology are described as "sticky" or "blunt" based on the shape...
chimera. rDNA technology uses palindromic sequences and leads to the production of stickyandbluntends. The DNA sequences used in the construction of recombinant...
relying on stickyends, the bluntend TOPO vector has bluntends where the topoisomerase molecules are bound. Commercial kits, such as the Zero Blunt® Cloning...
leaves no overhang, called a bluntend. Bluntends are much less likely to be ligated by a DNA ligase because the bluntend doesn't have the overhanging...
inserting a fragment into a plasmid vector would use about 0.01 (stickyends) to 1 (bluntends) units of ligase. The optimal incubation temperature for T4...
The ends are bluntand can be ligated into a blunt cloning site easily but with lower efficiency than stickyends. The structure of this enzyme, and several...
protospacer adjacent motif preference, and for creating sticky rather than bluntends at the cut site. These and other differences may make it more suitable...
it is used as a restriction enzyme. EcoRI creates 4 nucleotide stickyends with 5' end overhangs of AATT. The nucleic acid recognition sequence where...
restriction enzyme and the stickyends are ligated together into concatamers. Following concatenation, the fragments are ligated into plasmids and are used to...
Both the cDNA and the linker have bluntends which can be ligated together using a high concentration of T4 DNA ligase. Then stickyends are produced in...
short petioles, blades oval or ovate, on both ends acuminate or blunt. Stem erect, 90 – 150 cm long, sparse and inconspicuously warted, inflorescence paniculate...
contain either blunt ends or overhangs known as stickyends, and vector DNA and foreign DNA with compatible ends can then be joined by molecular ligation. After...
leaves usually have a few blunt teeth near their endsand flowers which are usually lilac-coloured. It is a distinctive and widespread species. Eremophila...
Argentipallium obtusifolium, commonly known as blunt everlasting, is a species of flowering plant in the family Asteraceae. It is a small, multi-stemmed...
and 5–10 mm (0.2–0.4 in) wide. They are sticky, glabrous and green with a few blunt teeth along their edges near the ends. The flowers are white and mauve...
into fragments. Not all restriction endonucleases cut symmetrically and leave bluntends like HindII described above. Many endonucleases cleave the DNA backbones...
Scrophulariaceae and is endemic to a small area in the south-west of Western Australia. It is an erect shrub with sticky, aromatic leaves and pink, blue or...
anxiety, paranoia and panic. The sticky resins of the fresh flowering female cannabis plant are collected. Traditionally this was, and still is, done in...
approaching opponents and hitting the right buttons. With Blunt Attack mode ON the samurai will use the blunt side of the blade, and as the result, enemies...
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with a bluntend. The flowers are white or cream-coloured and arranged singly in upper leaf axils on a stalk 1–2 mm (0.04–0.08 in) long andsticky. The...
(distinctly enlarged in the middle and tapered toward both ends) with blunt to somewhat sharp apices, often covered with mucilage, and measures 26–38 by 7–11 μm;...
polymerases) have 5'→3' exonuclease activity and generate an adenosine overhang (stickyends) at the 3' end of the newly generated strand. The Klenow fragment...
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