DNA recognition sequence of EcoRV. The green line represents the cut site.EcoRV cleaving DNA. The protein loosely binds DNA and scans for its recognition sequence. Once found, EcoRV kinks the DNA in a 50° angle and cleaves at the cognate sequence.
Restriction endonuclease EcoRV
EcoRV crystal structure complexed with double-stranded DNA
Identifiers
Symbol
Endonuc-EcoRV
Pfam
PF09233
InterPro
IPR015314
SCOP2
1sx5 / SCOPe / SUPFAM
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
EcoRV (pronounced "eco R five") is a type II restriction endonuclease isolated from certain strains of Escherichia coli. It has the alternative name Eco32I.
In molecular biology, it is a commonly used restriction enzyme. It creates blunt ends. The enzyme recognizes the palindromic 6-base DNA sequence 5'-GAT|ATC-3' and makes a blunt end at the vertical line.[1]
The complementary sequence is then 3'-CTA|TAG-5'. The ends are blunt and can be ligated into a blunt cloning site easily but with lower efficiency than sticky ends.
^Schildkraut I, Banner CD, Rhodes CS, Parekh S (1984). "The cleavage site for the restriction endonuclease EcoRV is 5'-GAT/ATC". Gene. 27 (3): 327–329. doi:10.1016/0378-1119(84)90078-7. PMID 6329909.
EcoRV (pronounced "eco R five") is a type II restriction endonuclease isolated from certain strains of Escherichia coli. It has the alternative name Eco32I...
restriction endonucleases, which cut DNA at specific sequences. For instance, the EcoRV enzyme shown to the left recognizes the 6-base sequence 5′-GATATC-3′ and...
with the chemical groups of the DNA. In the case of endonucleases such as EcoRV, BamHI, and PvuII, this nonspecific binding involves electrostatic interactions...
catalysis in EcoRV have led to the following proposed catalytic mechanism. It has been suggested that during the hydrolysis of DNA by EcoRV the catalytic...
solvents. EcoRII, another nuclease enzyme from E. coli. EcoRV, another nuclease enzyme from E. coli. Halford, S. E.; Johnson, N. P. (1980-11-01). "The EcoRI restriction...
processes. Some examples of type II restriction endonucleases include BamHI, EcoRI, EcoRV, HindIII, and HaeIII. Type III, however, cleaves the DNA at about 25...
common and have greater biological importance than mirror-like palindromes. EcoRI digestion produces "sticky" ends, whereas SmaI restriction enzyme cleavage...
June 2012). "Quiet on board please: science underway". ECOS (172). "New CSIRO research vessel RV Investigator arrives in Hobart after two-week voyage"...
from PCR amplification from a human embryo cDNA librarywas digested with EcoRV and XhoI and cloned into the SmaI and XhoI restriction site of the expression...
CIMMARON AIRE United States EJT Eclipse Aviation ECLIPSE JET United States ECQ Eco Air SKYBRIDGE Nigeria DEI Ecoair Algeria NAK École Nationale de l'Aviation...
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encampments and recreational vehicle (RV) parking by homeless persons who live in RVs. City law prohibits the use of eco-blocks on city streets, but as of...
wider, more streamlined, and roomier. A more efficient, direct-injected EcoTec3 V8 powertrain (5.3 for the Suburban, 6.2 for Yukon XL/Yukon Denali XL)...
RV Farley Mowat was a long-range, ice class ship. Originally built as a Norwegian fisheries research and enforcement vessel, she was purchased by the Sea...
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