This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. Find sources: "CDNA library" – news · newspapers · books · scholar · JSTOR(May 2008) (Learn how and when to remove this message)
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue-specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library.[1]
A cDNAlibrary is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of...
complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both...
amplifying a library of such in bacterial plasmids. In 1982–1983, the idea of selecting random or semi-random clones from such a cDNAlibrary for sequencing...
one-shot sequencing of a cloned cDNA. The cDNAs used for EST generation are typically individual clones from a cDNAlibrary. The resulting sequence is a...
monovalently). pVI has been widely used for the display of cDNAlibraries. The display of cDNAlibraries via phage display is an attractive alternative to the...
transcription. The early stages of transcriptome annotations began with cDNAlibraries published in the 1980s. Subsequently, the advent of high-throughput...
to screen against the W.bancrofti Larval stage 3 cDNAlibrary. This led to the identification of a cDNA sequence of W.bancrofti SXP-1 that encoded for a...
convert single-stranded RNA into double-stranded cDNA. In retroviruses and retrotransposons, this cDNA can then integrate into the host genome, from which...
stimulating activity, alpha (MGSA-α). CXCL1 was first cloned from a cDNAlibrary of genes induced by platelet-derived growth factor (PDGF) stimulation...
W.; Koehler, M. D.; Efstratiadis, A.; Maniatis, T. (1979). "Use of a cDNAlibrary for studies on evolution and developmental expression of the chorion...
assay, a genomic or cDNAlibrary is screened to identify the genetic sequence of a protein of interest. Expression cDNAlibraries may be screened with...
the CDNA 3 architecture, which is optimized for high-performance computing (HPC) and generative artificial intelligence (AI) workloads. The CDNA 3 architecture...
approaches were available. Libraries of silkmoth mRNA transcripts were collected and converted to complementary DNA (cDNA) for storage using reverse transcriptase...
clones is the cDNAlibrary. This generates a snap-shot of tissue mRNA that is stable and can be sequenced at a later stage. The cDNAlibrary must be sequenced...
obtain the cDNA for a specific gene, it is first necessary to construct a cDNAlibrary. The DNA of interest needs to be fragmented to provide a relevant DNA...
complementary DNA (cDNA) is synthesized, providing information about gene expression in the exact location of the sample. From the cDNA, libraries are synthesised...
be used to determine whether a particular DNA fragment is found in a cDNAlibrary. PCR has many variations, like reverse transcription PCR (RT-PCR) for...
nucleotides at the end) to create a cDNAlibrary for each sample, followed by PCR amplification using arbitrary 3′ primers for cDNA strand amplification together...
short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA (also called anti-sense RNA) sample (called target) under high-stringency...
"Isolation of markers for chondro-osteogenic differentiation using cDNAlibrary subtraction. Molecular cloning and characterization of a gene belonging...
cloned DNA sequence are also unnecessary when first making a genomic or cDNAlibrary of clones since the cloned genes are normally subcloned into a more appropriate...
Global Information