Artist's conception of DNA ligase repairing chromosomal damage
Identifiers
EC no.
6.5.1.1
CAS no.
9015-85-4
Databases
IntEnz
IntEnz view
BRENDA
BRENDA entry
ExPASy
NiceZyme view
KEGG
KEGG entry
MetaCyc
metabolic pathway
PRIAM
profile
PDB structures
RCSB PDB PDBe PDBsum
Gene Ontology
AmiGO / QuickGO
Search
PMC
articles
PubMed
articles
NCBI
proteins
ligase I, DNA, ATP-dependent
Identifiers
Symbol
LIG1
NCBI gene
3978
HGNC
6598
OMIM
126391
RefSeq
NM_000234
UniProt
P18858
Other data
Locus
Chr. 19 [1]
Search for
Structures
Swiss-model
Domains
InterPro
ligase III, DNA, ATP-dependent
Identifiers
Symbol
LIG3
NCBI gene
3980
HGNC
6600
OMIM
600940
RefSeq
NM_002311
UniProt
P49916
Other data
Locus
Chr. 17 q11.2-q12
Search for
Structures
Swiss-model
Domains
InterPro
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template,[1] with DNA ligase creating the final phosphodiester bond to fully repair the DNA.
DNA ligase is used in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Research applications). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA.
^Pascal JM, O'Brien PJ, Tomkinson AE, Ellenberger T (November 2004). "Human DNA ligase I completely encircles and partially unwinds nicked DNA". Nature. 432 (7016): 473–8. Bibcode:2004Natur.432..473P. doi:10.1038/nature03082. PMID 15565146. S2CID 3105417.
DNAligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role...
ligases include the word "ligase", such as in DNAligase, an enzyme commonly used in molecular biology laboratories to join together DNA fragments. However,...
discontinuously and later linked together by the enzyme DNAligase to create the lagging strand during DNA replication. They were discovered in the 1960s by...
enzyme, DNAligase, fragments generated by restriction enzymes could be joined in new combinations, termed recombinant DNA. By recombining DNA segments...
DNAligase 1 is an enzyme that in humans is encoded by the LIG1 gene. DNAligase I is the only known eukaryotic DNAligase involved in both DNA replication...
ability of a cell to alter its genetics by taking up extracellular ("naked") DNA from its environment in the process called transformation. Competence may...
eukaryotes, newly synthesized lagging-strand DNA transiently contains nicks (before being sealed by DNAligase) and provides a signal that directs mismatch...
joining (MMEJ), and homologous recombination (HR): In NHEJ, DNALigase IV, a specialized DNAligase that forms a complex with the cofactor XRCC4, directly...
of a DNA strand with no attached complement) known as a sticky end on each end of AATT. The overhang can then be used to ligate in (see DNAligase) a piece...
strand DNA replication. DNAligase III along with its cofactor XRCC1 catalyzes the nick-sealing step in short-patch BER in humans. DNAligase I ligates...
can be joined by DNAligase. There are hundreds of restriction endonucleases known, each attacking a different restriction site. The DNA fragments cleaved...
artificial DNA primers can be used to start DNA synthesis at known sequences in a template DNA molecule. Polymerase chain reaction (PCR), ligase chain reaction...
involvement of various polymerases, primers, and/or ligases. During the replication of DNA, for example, the DNA polymerase I leaves behind a hole between the...
MJ, Weinfeld M (January 2015). "DNAligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair". Nucleic Acids...
gene. XRCC1 is involved in DNA repair, where it complexes with DNAligase III. XRCC1 is involved in the efficient repair of DNA single-strand breaks formed...
and DNA fingerprinting. Enzymes called DNAligases can rejoin cut or broken DNA strands. Ligases are particularly important in lagging strand DNA replication...
Final ligation to complete NER and form a double stranded DNA is carried out by DNAligase. NER can be divided into two subpathways: global genomic NER...
The ligase chain reaction (LCR) is a method of DNA amplification. The ligase chain reaction (LCR) is an amplification process that differs from PCR in...
oligonucleotide annealing, the synthesis step relies on a set of thermostable DNAligase and polymerase enzymes. To date, several methods for gene synthesis have...
second component involves the bridging of DNA to DNALigase IV (LigIV), by XRCC4, with the aid of Cernunnos-XLF. DNA-PKcs and XRCC4 are anchored to Ku70 /...
RNase H to nick mRNA that is replaced with E. Coli DNA Polymerase I and sealed with E. Coli DNALigase. An optimization of this procedure relies on low...
during storage. Upon germination, seeds of Vicia faba undergo DNA repair. A plant DNAligase that is involved in repair of single- and double-strand breaks...
adjacent DNA fragments can anneal. The DNA polymerase incorporates nucleotides to fill in any gaps. The DNAligase covalently joins the DNA of adjacent...
with the help from T4 DNAligase. This is not a standard T4 DNAligase ligation reaction, where two pieces of double stranded DNA are ligated together...
Blunt ends are not always desired in biotechnology since when using a DNAligase to join two molecules into one, the yield is significantly lower with...
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