Restriction sites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length[1]) sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences[2] (because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby.
^Russell, Peter J. (2006). iGenetics: A Mendelian Approach. Benjamin Cummings. ISBN 978-0805346664.
^Lehninger, Albert L.; Nelson, David L.; Cox, Michael M. (2008). Principles of Biochemistry (5th ed.). New York, NY: W.H. Freeman and Company. p. 305–306. ISBN 978-0-7167-7108-1.
Restrictionsites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides...
recognition sites within molecules known as restrictionsites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes...
polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences...
Restrictionsite associated DNA (RAD) markers are a type of genetic marker which are useful for association mapping, QTL-mapping, population genetics,...
does not have a Type IIS restriction enzyme recognition site, the correctly-ligated product cannot be cut again by the restriction enzyme, meaning the reaction...
well. In a restriction digest, DNA molecules are cleaved at specific restrictionsites of 4-12 nucleotides in length by use of restriction enzymes which...
of physical mapping are fluorescent in situ hybridization (FISH), restrictionsite mapping and sequencing by clones. The goal of physical mapping, as...
multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restrictionsites - a standard feature...
either blunt or overhung ends, either at or nearby the recognition site. Restriction enzymes are quite variable in the short DNA sequences they recognize...
A restriction map is a map of known restrictionsites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular...
for profiling of microbial communities based on the position of a restrictionsite closest to a labelled end of an amplified gene. The method is based...
sequence recognized for cleavage by a restriction enzyme is called the restrictionsite. Typically, a restrictionsite will be a palindromic sequence about...
complementary to the adaptor sequence, the restrictionsite sequence and a few nucleotides inside the restrictionsite fragments (as described in detail below)...
isolated examples of the two types of enzymes responsible for phage growth restriction in Escherichia coli (E. coli) bacteria. One of these enzymes added a...
process called restriction. Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restrictionsite, and cutting both...
microalgae. Site-specific recombination makes use of phage integrases instead of restriction enzymes, eliminating the need for having restrictionsites in the...
cloning schemes, as it requires fewer steps and fewer reagents. No restrictionsite scar remains between two DNA fragments, but the region between the...
used in plant breeding. Although GBS presents an approach similar to restriction-site-associated DNA sequencing (RAD-seq) method, they differ in some substantial...
unmethylated forms of restrictionsites. In contrast, the other restriction enzyme can recognize only the unmethylated form of the restrictionsite. This property...
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the...
vector is treated with a restriction endonuclease to cleave the DNA at the site where foreign DNA will be inserted. The restriction enzyme is chosen to generate...
random occurrence of a Restrictionsite, a 4-bp sequence can occur once every 256 bp. A restriction enzyme having a recognition site of more than 4 bp would...
Erlich, "Enzymatic Amplification of β-globin Genomic Sequences and RestrictionSite Analysis for Diagnosis of Sickle Cell Anemia"—the polymerase chain...
restrictionsites for more than forty restriction enzymes. Eleven of these forty sites lie within the TetR gene. There are two sites for restriction enzymes...
amplification is directed across the altered restrictionsite, and the products digested with the restriction enzyme. When fractionated by agarose or polyacrylamide...
example through the presence of restrictionsites. The vector and the foreign DNA may be treated with a restriction enzyme that cuts the DNA, and DNA...
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