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Sample preparation information


In analytical chemistry, sample preparation (working-up) refers to the ways in which a sample is treated prior to its analyses. Preparation is a very important step in most analytical techniques, because the techniques are often not responsive to the analyte in its in-situ form, or the results are distorted by interfering species. Sample preparation may involve dissolution, extraction, reaction with some chemical species, pulverizing, treatment with a chelating agent (e.g. EDTA), masking, filtering, dilution, sub-sampling or many other techniques. Treatment is done to prepare the sample into a form ready for analysis by specified analytical equipment. Sample preparation could involve: crushing and dissolution, chemical digestion with acid or alkali, sample extraction, sample clean up and sample pre-concentration.

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Sample preparation

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analytical chemistry, sample preparation (working-up) refers to the ways in which a sample is treated prior to its analyses. Preparation is a very important...

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Sample preparation in mass spectrometry

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Sample preparation for mass spectrometry is used for the optimization of a sample for analysis in a mass spectrometer (MS). Each ionization method has...

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Sample preparation equipment

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Sample preparation equipment refers to equipment used for the preparation of physical specimens for subsequent microscopy or related disciplines - including...

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Miltenyi Biotec

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and clinical applications. These services include techniques of sample preparation, cell separation, cell sorting, flow cytometry, spatial biology, cell...

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Focused ion beam

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modification, photomask repair and transmission electron microscope (TEM) sample preparation of site specific locations on integrated circuits have become commonplace...

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Infrared spectroscopy

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clinical applications is the high sensitivity to technical equipment and sample preparation techniques, which makes it difficult to construct large-scale databases...

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Electron microscope

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a sample rather than its interior, the electrons do not have to travel through the sample. This reduces the need for extensive sample preparation to...

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Sanger sequencing

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fluorescently labelled for detection in automated sequencing machines. The DNA sample is divided into four separate sequencing reactions, containing all four...

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Supercritical fluid chromatography

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Supercritical fluid chromatography (SFC) is a form of normal phase chromatography that uses a supercritical fluid such as carbon dioxide as the mobile...

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MALDI imaging

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analytes at one time without destroying the sample, make it a useful method in tissue-based study. Sample preparation is a critical step in imaging spectroscopy...

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Van der Pauw method

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coefficient of a sample. Its power lies in its ability to accurately measure the properties of a sample of any arbitrary shape, as long as the sample is approximately...

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Histology

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laboratory technicians, and biomedical scientists. Most histological samples need preparation before microscopic observation; these methods depend on the specimen...

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Peptide mass fingerprinting

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and are ready to be analyzed. There is one predominantly MALDI-MS sample preparation technique, namely dried droplet technique. The target is inserted...

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Inductively coupled plasma mass spectrometry

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techniques for analysis of glass due to the short time for sample preparation and sample, small sample size of less than 250 nanograms. In addition there is...

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Fluorescence microscope

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order for a sample to be suitable for fluorescence microscopy it must be fluorescent. There are several methods of creating a fluorescent sample; the main...

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Transmission electron microscopy

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to the TEM technique. Many materials require extensive sample preparation to produce a sample thin enough to be electron transparent, which makes TEM...

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Bioanalysis

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often deal with large numbers of samples, for example resulting from clinical trials. As such, automated sample preparation methods and liquid-handling robots...

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Oedometer test

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also provides background information and best-practice advice on sample preparation for various geotechnical investigations. There are also two ISO standards...

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Homogenizer

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aerosols, risk of infection, or noise. Homogenization is a very common sample preparation step prior to the analysis of nucleic acids, proteins, cells, metabolism...

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DNA sequencing

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challenge for sample preparation method to scale-up. Several liquid handling instruments are being used for the preparation of higher numbers of samples with a...

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Scanning electron microscope

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best light microscopes. SEM samples have to be small enough to fit on the specimen stage, and may need special preparation to increase their electrical...

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Immunohistochemistry

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to be tested. Then acetone or NBF can be used. Sectioning of the tissue sample is done using a microtome. For paraffin embedded tissue 4 μm is normal thickness...

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Bismuth ferrite

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a mortar or by ball milling and then fired at elevated temperatures. Preparation of pure stoichiometric BiFeO3 is challenging due to the volatility of...

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Environmental monitoring

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Diffusion Sampling Devices for Monitoring Ambient Air Concentrations". In Barcelo, D. (ed.). Monitoring of Air Pollutants: Sampling, Sample Preparation and...

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Microtome

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Important in science, microtomes are used in microscopy for the preparation of samples for observation under transmitted light or electron radiation. Microtomes...

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Western blot

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completing the sample preparation, the protein content is ready to be separated by the utilization of gel electrophoresis. The proteins of the sample are separated...

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Certified reference materials

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reference material typically include: Collection or synthesis of material Sample preparation (including homogenization, stabilization, bottling etc.) Homogeneity...

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