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Salting out information


Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out)[1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength. Salting out is typically used to precipitate large biomolecules, such as proteins or DNA.[2] Because the salt concentration needed for a given protein to precipitate out of the solution differs from protein to protein, a specific salt concentration can be used to precipitate a target protein. This process is also used to concentrate dilute solutions of proteins. Dialysis can be used to remove the salt if needed.

  1. ^ Genck, Wayne (2010). "Make The Most of Antisolvent Crystallization". Chemical Processing. PutmanMedia.
  2. ^ Chacon-Cortes, D; Griffiths, L (4 December 2020). "Methods for extracting genomic DNA from whole blood samples: current perspectives". Journal of Biorepository Science for Applied Medicine. 2014 (2): 1–9. doi:10.2147/BSAM.S46573.

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Salting out

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Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out)...

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Salting in

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proteins drop sharply and proteins can precipitate out, referred to as "salting out". Initial salting in at low concentrations is explained by the Debye–Huckel...

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Protein precipitation

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protein to fall out of solution (aggregate and precipitate). Salting out is a spontaneous process when the right concentration of the salt is reached in...

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Salting

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Look up salting in Wiktionary, the free dictionary. Salting or Salted may refer to: George Salting (1835–1909), Australian-born English art collector,...

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Ammonium sulfate precipitation

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increases with increasing salt concentration, an effect termed "salting in". As the salt concentration is further increased, the solubility of the protein...

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Sodium chloride

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casings. In textiles and dyeing, salt is used as a brine rinse to separate organic contaminants, to promote "salting out" of dyestuff precipitates, and...

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Isopropyl alcohol

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Notably, it is not miscible with salt solutions and can be separated by adding sodium chloride in a process known as salting out. It forms an azeotrope with...

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Hofmeister series

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nonpolar molecules ("salting in") and decrease the order in water; in effect, they weaken the hydrophobic effect. The "salting out" effect is commonly...

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Ammonium sulfate

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and precipitate out of the solution in a concentrated form. This method is called salting out and necessitates the use of high salt concentrations that...

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Poloxamer

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ability to act as water structure makers (salting-out) or water structure breakers (salting-in). Salting-out salts increase the self-hydration of water...

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Saltwater soap

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of salt (sodium chloride) in seawater lowers the solubility of soaps made with sodium hydroxide, due to the common ion effect, a form of salting out. Potassium...

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Salted fish

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Salted fish, such as kippered herring or dried and salted cod, is fish cured with dry salt and thus preserved for later eating. Drying or salting, either...

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Salt

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unpalatable food. Salting, brining, and pickling are also ancient and important methods of food preservation. Some of the earliest evidence of salt processing...

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Kosmotropic

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the solution. Ammonium sulfate is the traditional kosmotropic salt for the salting out of protein from an aqueous solution. Kosmotropes are used to induce...

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Hydrogel

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hydrogel. This method, called "salting out", has been applied to poly(vinyl alcohol) hydrogels by adding a sodium sulfate salt solution. Some of these processing...

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Ionic strength

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equations Poisson–Boltzmann equation Specific ion Interaction Theory Salting in Salting out Ionic strength Ionic strength introduction at the EPA web site Solomon...

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Protein purification

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isolated protein has to be confirmed by an independent method. Salting in Salting out Protein tag Protein production Host cell protein Berg, Jeremy M...

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Hard soap

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hard soap production. After adding sodium chloride (a process known as salting out), the soap nucleus rises and separates. The water-soluble glycerin and...

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Integral membrane protein

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performing the extraction including the uses of "detergents, low ionic salt (salting out), shearing force, and rapid pressure change". The Protein Structure...

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Ethanol precipitation

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using isopropanol. DNA extraction Phenol–chloroform extraction Salting in Salting out SCODA DNA purification Spin column-based nucleic acid purification...

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Separatory funnel

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this process, a small amount of saturated saline solution is added ("salting out"). Research is being done on alternative, more efficient techniques,...

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Hydrotrope

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additive K is the salting coefficient, which is a measure of the sensitivity of the activity coefficient of the solute towards the salt. Hydrotropes are...

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Electrophoretic deposition

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and the resultant decrease in solubility. Concentration coagulation. Salting out. The primary electrochemical process which occurs during aqueous electrodeposition...

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Nuclear reprocessing

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mechanism. This process has the disadvantage of requiring the use of a salting-out reagent (aluminium nitrate) to increase the nitrate concentration in...

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Surfactant

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meaning. A common example of surface tension increase is salting out: adding an inorganic salt to an aqueous solution of a weakly polar substance will...

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Activity coefficient

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For uncharged species, the activity coefficient γ0 mostly follows a salting-out model: log 10 ⁡ ( γ 0 ) = b I {\displaystyle \log _{10}(\gamma _{0})=bI}...

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