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Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out)[1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength. Salting out is typically used to precipitate large biomolecules, such as proteins or DNA.[2] Because the salt concentration needed for a given protein to precipitate out of the solution differs from protein to protein, a specific salt concentration can be used to precipitate a target protein. This process is also used to concentrate dilute solutions of proteins. Dialysis can be used to remove the salt if needed.
^Genck, Wayne (2010). "Make The Most of Antisolvent Crystallization". Chemical Processing. PutmanMedia.
^Chacon-Cortes, D; Griffiths, L (4 December 2020). "Methods for extracting genomic DNA from whole blood samples: current perspectives". Journal of Biorepository Science for Applied Medicine. 2014 (2): 1–9. doi:10.2147/BSAM.S46573.
Saltingout (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out)...
proteins drop sharply and proteins can precipitate out, referred to as "saltingout". Initial salting in at low concentrations is explained by the Debye–Huckel...
protein to fall out of solution (aggregate and precipitate). Saltingout is a spontaneous process when the right concentration of the salt is reached in...
Look up salting in Wiktionary, the free dictionary. Salting or Salted may refer to: George Salting (1835–1909), Australian-born English art collector,...
increases with increasing salt concentration, an effect termed "salting in". As the salt concentration is further increased, the solubility of the protein...
casings. In textiles and dyeing, salt is used as a brine rinse to separate organic contaminants, to promote "saltingout" of dyestuff precipitates, and...
Notably, it is not miscible with salt solutions and can be separated by adding sodium chloride in a process known as saltingout. It forms an azeotrope with...
nonpolar molecules ("salting in") and decrease the order in water; in effect, they weaken the hydrophobic effect. The "saltingout" effect is commonly...
and precipitate out of the solution in a concentrated form. This method is called saltingout and necessitates the use of high salt concentrations that...
ability to act as water structure makers (salting-out) or water structure breakers (salting-in). Salting-out salts increase the self-hydration of water...
of salt (sodium chloride) in seawater lowers the solubility of soaps made with sodium hydroxide, due to the common ion effect, a form of saltingout. Potassium...
unpalatable food. Salting, brining, and pickling are also ancient and important methods of food preservation. Some of the earliest evidence of salt processing...
Salted fish, such as kippered herring or dried and salted cod, is fish cured with dry salt and thus preserved for later eating. Drying or salting, either...
the solution. Ammonium sulfate is the traditional kosmotropic salt for the saltingout of protein from an aqueous solution. Kosmotropes are used to induce...
equations Poisson–Boltzmann equation Specific ion Interaction Theory Salting in Saltingout Ionic strength Ionic strength introduction at the EPA web site Solomon...
hydrogel. This method, called "saltingout", has been applied to poly(vinyl alcohol) hydrogels by adding a sodium sulfate salt solution. Some of these processing...
using isopropanol. DNA extraction Phenol–chloroform extraction Salting in Saltingout SCODA DNA purification Spin column-based nucleic acid purification...
hard soap production. After adding sodium chloride (a process known as saltingout), the soap nucleus rises and separates. The water-soluble glycerin and...
isolated protein has to be confirmed by an independent method. Salting in Saltingout Protein tag Protein production Host cell protein Berg, Jeremy M...
performing the extraction including the uses of "detergents, low ionic salt (saltingout), shearing force, and rapid pressure change". The Protein Structure...
additive K is the salting coefficient, which is a measure of the sensitivity of the activity coefficient of the solute towards the salt. Hydrotropes are...
meaning. A common example of surface tension increase is saltingout: adding an inorganic salt to an aqueous solution of a weakly polar substance will...
this process, a small amount of saturated saline solution is added ("saltingout"). Research is being done on alternative, more efficient techniques,...
mechanism. This process has the disadvantage of requiring the use of a salting-out reagent (aluminium nitrate) to increase the nitrate concentration in...
crystals may form. Microdialysis can produce crystals by saltingout, employing high concentrations of salt or other small membrane-permeable compounds that decrease...
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