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Ethanol precipitation information


Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA, and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding ethanol as an antisolvent.

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Ethanol precipitation

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Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA, and polysaccharides such as pectin and xyloglucan from aqueous solutions...

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Plasmid preparation

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biomolecules precipitate, allowing the nucleic acid to be separated. Ethanol precipitation is a widely used method for purifying and concentrating nucleic...

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Ethanol

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Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula CH3CH2OH. It...

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DNA extraction

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lysis step. The most commonly used procedures are: Ethanol precipitation usually by ice-cold ethanol or isopropanol. Since DNA is insoluble in these alcohols...

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Polyacrylamide

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(6): 958–65. PMID 13665142. Gaillard C, Strauss F (January 1990). "Ethanol precipitation of DNA with linear polyacrylamide as carrier". Nucleic Acids Research...

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Sodium acetate

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acetate is also useful for increasing yields of DNA isolation by ethanol precipitation. Sodium acetate is used in the textile industry to neutralize sulfuric...

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Cellulosic ethanol

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Cellulosic ethanol is ethanol (ethyl alcohol) produced from cellulose (the stringy fiber of a plant) rather than from the plant's seeds or fruit. It can...

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Chromatography in blood processing

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in a definite direction. Cold ethanol precipitation, developed by Cohn in 1946, manipulates pH, ionic strength, ethanol concentration and temperature...

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RNA extraction

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bare skin touch the equipment. Column purification DNA extraction Ethanol precipitation Phenol-chloroform extraction Peirson SN, Butler JN (2007). "RNA...

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Denatured alcohol

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denaturant would interfere. In molecular biology, denatured ethanol should not be used for the precipitation of nucleic acids, since the additives may interfere...

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Protein precipitation

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Cohn process for plasma protein fractionation relies on solvent precipitation with ethanol to isolate individual plasma proteins. a clinical application...

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Oligonucleotide synthesis

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of methods. Most commonly, the crude product is desalted using ethanol precipitation, size exclusion chromatography, or reverse-phase HPLC. To eliminate...

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Restriction map

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proteins and other molecules. Then the DNA can be subjected to ethanol precipitation to concentrate the sample. Vector NTI, bioinformatics software used...

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SYBR Gold

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unbound dye. SYBR Gold can be readily removed from nucleic acids by ethanol precipitation, leaving pure templates available for subsequent manipulation or...

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Comparative genomic hybridization

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aqueous layer is removed and further treated using ether and finally ethanol precipitation is used to concentrate the DNA. May be completed using DNA isolation...

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Surveyor nuclease assay

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standard extraction methods such as Proteinase K digestion followed by ethanol precipitation or by other commercially available methods. If the DNA is predicted...

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Cohn process

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by reducing the consumption of ethanol. Instead of 40% in certain steps, Gerlough used 20% ethanol for precipitation. This is especially used for Fractions...

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Boom method

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Chaotropic agent DNA extraction DNA separation by silica adsorption Ethanol precipitation Minicolumn purification Nucleic acid methods Phenol-chloroform extraction...

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Halostagnicola larsenii

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nucleic extraction by an organic solvent, and DNA recovery by ethanol precipitation were used. The 16S ribosomal RNA gene sequence of H. larsenii was...

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Electroelution

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buffer and purified, using phenol–chloroform extraction followed by ethanol precipitation. This method is simple, rapid and yields high recovery (75%) of...

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Still

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beverages containing ethanol. Since ethanol boils at a much lower temperature than water, simple distillation can separate ethanol from water by applying...

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