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Ammonium sulfate precipitation information


Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory scale protein purification and fractionation that can be used to separate proteins by altering their solubility in the presence of a high salt concentration.

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Ammonium sulfate

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and glufosinate herbicides. Ammonium sulfate precipitation is a common method for protein purification by precipitation. As the ionic strength of a solution...

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Ammonium sulfate precipitation

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Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory scale protein purification and fractionation that can...

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Biuret test

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this assay inappropriate for protein samples purified from ammonium sulfate precipitation. Due to its insensitivity and little interference by free amino...

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Ammonium chlorate

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with ammonium carbonate or ammonium sulfate, producing the respective carbonate or sulfate precipitate and an ammonium chlorate solution. Ammonium chlorate...

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Ammonium nitrite

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peroxide, or in a precipitation reaction of barium or lead nitrite with ammonium sulfate, or silver nitrite with ammonium chloride, or ammonium perchlorate...

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Salting out

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chromatography may be required. Ionic strength Protein precipitation Salting in Ammonium sulfate precipitation Hofmeister series Genck, Wayne (2010). "Make The...

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Protein purification

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isolate proteins is precipitation with ammonium sulfate (NH4)2SO4. This is performed by adding increasing amounts of ammonium sulfate and collecting the...

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Quaternary ammonium cation

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strips, the sulfate salts are often used. Older aluminium electrolytic capacitors and spermicidal jellies also contain quaternary ammonium salts. Quats...

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Ammonium bicarbonate

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(NH4)HCO3}}} Since ammonium bicarbonate is thermally unstable, the reaction solution is kept cold, which allows the precipitation of the product as white...

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Diammonium phosphate

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2012-12-10. George, C.W.; Susott, R.A. (April 1971). "Effects of Ammonium Phosphate and Sulfate on the Pyrolysis and Combustion of Cellulose". Research Paper...

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Hofmeister series

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commonly exploited in protein purification through the use of ammonium sulfate precipitation. However, these salts also interact directly with proteins (which...

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Microbiologically induced calcite precipitation

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that can induce the carbonate precipitation are photosynthetic microorganisms such as cyanobacteria and microalgae; sulfate-reducing bacteria; and some...

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Centrifugation

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proteins using purification techniques such as salting out, e.g. ammonium sulfate precipitation. Centrifugation is also an important technique in waste treatment...

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Protein precipitation

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used to precipitate a protein. Addition of a neutral salt, such as ammonium sulfate, compresses the solvation layer and increases protein–protein interactions...

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Ammonium metavanadate

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hydrogen bonds with ammonium ions. Vanadium is often purified from aqueous extracts of slags and ore by selective precipitation of ammonium metavanadate. The...

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Phycocyanin

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Then, C-PC is isolated with centrifugation and purified with ammonium sulfate precipitation or chromatography -either ion or gel-filtration. After, the...

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Keyhole limpet hemocyanin

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Megathura crenulata by a series of steps that typically includes ammonium sulfate precipitation and dialysis, and may involve chromatographic purification to...

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List of alchemical substances

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copper(II) sulfate pentahydrate. Green vitriol – a mineral; iron(II) sulfate heptahydrate. (or ferrous sulfate) Red vitriol - cobalt sulfate. Sweet vitriol...

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Herapathite

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herapathite consists of quinine (in a cationic doubly-protonated ammonium form), sulfate counterions, and triiodide units, all as a hydrate. They combine...

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Mallein test

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Purification of malleo proteins Trichloroacetic acid (TCA) preparation Ammonium sulfate precipitation Gel filtration chromatography Sensitization of animals Potency...

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Barium hydroxide

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sulfate and barium phosphate with sulfuric and phosphoric acids, respectively. Reaction with hydrogen sulfide produces barium sulfide. Precipitation of...

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Thermometric titration

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the determination of sulfate in brine (including electrolysis brines), in nickel refining solutions and particularly for sulfate in wet process phosphoric...

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Solubility chart

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are insoluble, unless the compound includes group 1 elements or ammonium. "Ammonium oxide" does not exist. However, its theoretical molecular formula...

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Kosmotropic

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kosmotropes. Chaotropic agent and guanidinium chloride Protein precipitation, on ammonium sulfate "salting out" Moelbert S, Normand B, De Los Rios P (2004)...

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Cobalt extraction

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autoclave and sulfuric acid added to remove nickel as nickel(II) sulfate-ammonium sulfate hexahydrate ([NiSO4]•[(NH4)2SO4]•6H2O) which is then sent to have...

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Lysis buffer

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functions). Ionic detergents like sodium dodecyl sulfate (SDS) and cationic detergents like ethyl trimethyl ammonium bromide are denaturing (will disrupt protein...

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