The blue bottle experiment is a color-changing redox chemical reaction. An aqueous solution containing glucose, sodium hydroxide, methylene blue is prepared in a closed bottle containing some air. Upon standing, it spontaneously turns from blue to colorless due to reduction of methylene blue by the alkaline glucose solution. However, shaking the bottle oxidizes methylene blue back into its blue form. With further shaking, this color-change cycle can be repeated many times.[1] This experiment is a classic chemistry demonstration that can be used in laboratory courses as a general chemistry experiment to study chemical kinetics and reaction mechanism.[2] The reaction also works with other reducing agents besides glucose[3] and other redox indicator dyes besides methylene blue.[4]
^Baker, Colin (1 November 2006). "The 'blue bottle' reaction". Education in Chemistry. Vol. 43, no. 6. Royal Society of Chemistry. p. 155.
^Engerer, Steven C.; Cook, A. Gilbert (1999). "The Blue Bottle Reaction as a General Chemistry Experiment on Reaction Mechanisms". Journal of Chemical Education. 76 (11): 1519–1520. Bibcode:1999JChEd..76.1519E. doi:10.1021/ed076p1519.
^Cook, A. Gilbert; Tolliver, Randi M.; Williams, Janelle E. (1994). "The Blue Bottle Experiment Revisited: How Blue? How Sweet?". Journal of Chemical Education. 71 (2): 160. Bibcode:1994JChEd..71..160C. doi:10.1021/ed071p160.
^Limpanuparb, Taweetham; Areekul, Cherprang; Montriwat, Punchalee; Rajchakit, Urawadee (2017). "Blue Bottle Experiment: Learning Chemistry without Knowing the Chemicals". Journal of Chemical Education. 94 (6): 730. Bibcode:2017JChEd..94..730L. doi:10.1021/acs.jchemed.6b00844.
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