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Restriction fragment length polymorphism information


In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. The term may refer to a polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences can be illustrated. In RFLP analysis, a DNA sample is digested into fragments by one or more restriction enzymes, and the resulting restriction fragments are then separated by gel electrophoresis according to their size.

RFLP analysis is now largely obsolete due to the emergence of inexpensive DNA sequencing technologies, but it was the first DNA profiling technique inexpensive enough to see widespread application. RFLP analysis was an important early tool in genome mapping, localization of genes for genetic disorders, determination of risk for disease, and paternity testing.

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Restriction fragment length polymorphism

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biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations...

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Amplified fragment length polymorphism

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Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic...

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Terminal restriction fragment length polymorphism

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Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based...

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Restriction fragment mass polymorphism

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Restriction Fragment Mass Polymorphism (RFMP) is a technology which digests DNA into oligonucleotide fragments, and detects variation of DNA sequences...

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SNP genotyping

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employed to generate high-fidelity SNP genotyping methods. Restriction fragment length polymorphism (RFLP) is considered to be the simplest and earliest method...

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Restriction digest

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techniques: RFLP – Restriction fragment length polymorphism AFLP – Amplified fragment length polymorphism STRP – Short tandem repeat polymorphism There are numerous...

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DNA profiling

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stands for restriction fragment length polymorphism and, in terms of DNA analysis, describes a DNA testing method which utilizes restriction enzymes to...

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STR analysis

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method differs from restriction fragment length polymorphism analysis (RFLP) since STR analysis does not cut the DNA with restriction enzymes. Instead,...

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Barreleye

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species have been isolated and cultured in the lab. Through restriction fragment length polymorphism analysis, the two strains have been shown to differ only...

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Community fingerprinting

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fingerprinting. Terminal restriction fragment length polymorphism (T-RFLP) is a method that uses fluorescently-labeled DNA fragments to produce a community...

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Restriction enzyme

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gene mutations (polymorphisms) have occurred within a population. The latter example is called restriction fragment length polymorphism (RFLP). Artificial...

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Polysomy

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properties. Restriction enzymes cut DNA at a specific site and the DNA fragments that are left are called restriction fragment length polymorphisms, or RFLPs...

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Restriction site associated DNA markers

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are other restriction site marker techniques, like RFLP or amplified fragment length polymorphism (AFLP), which use fragment length polymorphism caused by...

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DNA paternity testing

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testing are using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Paternity testing can now also be performed while...

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Restriction site

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enzyme. Restriction sites can be used for multiple applications in molecular biology such as identifying restriction fragment length polymorphisms (RFLPs)...

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DNA bank

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for testing and analyzing genetic information including restriction fragment length polymorphism (RFLP) and polymerase chain reactions (PCR). DNA banking...

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Molecular marker

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studies. In genetics, a molecular marker (identified as genetic marker) is a fragment of DNA that is associated with a certain location within the genome. Molecular...

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Cleaved amplified polymorphic sequence

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analysis of genetic markers. It is an extension to the restriction fragment length polymorphism (RFLP) method, using polymerase chain reaction (PCR) to...

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Armillaria gallica

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with an estimated age of 1,500 years. The analysis used restriction fragment length polymorphism (RFLP) and random amplification of polymorphic DNA (RAPD)...

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Felidae

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three evolutionary lineages of Felidae using mitochondrial restriction fragment length polymorphism variation". Journal of Mammalian Evolution. 3 (2): 97–120...

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Sardine

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Retrieved 11 December 2018. Grant, W. S.; et al. (1998). "Why restriction fragment length polymorphism analysis of mitochondrial DNA failed to resolve sardine...

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Genotyping

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of an individual. Current methods of genotyping include restriction fragment length polymorphism identification (RFLPI) of genomic DNA, random amplified...

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Amplified Ribosomal DNA Restriction Analysis

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Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the...

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Forensic DNA analysis

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When DNA analysis was first discovered, a process called Restriction Fragment Length Polymorphism (RFLP) was used to analyze DNA. However, RFLP was an inefficient...

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Bulked segregant analysis

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samples can then be analysed using techniques such as Restriction fragment length polymorphism or RAPD to detect similarities and differences in the various...

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