Restriction fragment length polymorphism information
Molecular biology technique
In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. The term may refer to a polymorphism itself, as detected through the differing locations of restriction enzyme sites, or to a related laboratory technique by which such differences can be illustrated. In RFLP analysis, a DNA sample is digested into fragments by one or more restriction enzymes, and the resulting restriction fragments are then separated by gel electrophoresis according to their size.
RFLP analysis is now largely obsolete due to the emergence of inexpensive DNA sequencing technologies, but it was the first DNA profiling technique inexpensive enough to see widespread application. RFLP analysis was an important early tool in genome mapping, localization of genes for genetic disorders, determination of risk for disease, and paternity testing.
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biology, restrictionfragmentlengthpolymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations...
Amplified fragmentlengthpolymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic...
Terminal restrictionfragmentlengthpolymorphism (TRFLP or sometimes T-RFLP) is a molecular biology technique for profiling of microbial communities based...
RestrictionFragment Mass Polymorphism (RFMP) is a technology which digests DNA into oligonucleotide fragments, and detects variation of DNA sequences...
employed to generate high-fidelity SNP genotyping methods. Restrictionfragmentlengthpolymorphism (RFLP) is considered to be the simplest and earliest method...
techniques: RFLP – Restrictionfragmentlengthpolymorphism AFLP – Amplified fragmentlengthpolymorphism STRP – Short tandem repeat polymorphism There are numerous...
stands for restrictionfragmentlengthpolymorphism and, in terms of DNA analysis, describes a DNA testing method which utilizes restriction enzymes to...
method differs from restrictionfragmentlengthpolymorphism analysis (RFLP) since STR analysis does not cut the DNA with restriction enzymes. Instead,...
species have been isolated and cultured in the lab. Through restrictionfragmentlengthpolymorphism analysis, the two strains have been shown to differ only...
fingerprinting. Terminal restrictionfragmentlengthpolymorphism (T-RFLP) is a method that uses fluorescently-labeled DNA fragments to produce a community...
gene mutations (polymorphisms) have occurred within a population. The latter example is called restrictionfragmentlengthpolymorphism (RFLP). Artificial...
properties. Restriction enzymes cut DNA at a specific site and the DNA fragments that are left are called restrictionfragmentlengthpolymorphisms, or RFLPs...
are other restriction site marker techniques, like RFLP or amplified fragmentlengthpolymorphism (AFLP), which use fragmentlengthpolymorphism caused by...
testing are using polymerase chain reaction (PCR) and restrictionfragmentlengthpolymorphism (RFLP). Paternity testing can now also be performed while...
enzyme. Restriction sites can be used for multiple applications in molecular biology such as identifying restrictionfragmentlengthpolymorphisms (RFLPs)...
for testing and analyzing genetic information including restrictionfragmentlengthpolymorphism (RFLP) and polymerase chain reactions (PCR). DNA banking...
studies. In genetics, a molecular marker (identified as genetic marker) is a fragment of DNA that is associated with a certain location within the genome. Molecular...
analysis of genetic markers. It is an extension to the restrictionfragmentlengthpolymorphism (RFLP) method, using polymerase chain reaction (PCR) to...
with an estimated age of 1,500 years. The analysis used restrictionfragmentlengthpolymorphism (RFLP) and random amplification of polymorphic DNA (RAPD)...
three evolutionary lineages of Felidae using mitochondrial restrictionfragmentlengthpolymorphism variation". Journal of Mammalian Evolution. 3 (2): 97–120...
Retrieved 11 December 2018. Grant, W. S.; et al. (1998). "Why restrictionfragmentlengthpolymorphism analysis of mitochondrial DNA failed to resolve sardine...
of an individual. Current methods of genotyping include restrictionfragmentlengthpolymorphism identification (RFLPI) of genomic DNA, random amplified...
Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restrictionfragmentlengthpolymorphism) to the gene encoding the...
When DNA analysis was first discovered, a process called RestrictionFragmentLengthPolymorphism (RFLP) was used to analyze DNA. However, RFLP was an inefficient...
samples can then be analysed using techniques such as Restrictionfragmentlengthpolymorphism or RAPD to detect similarities and differences in the various...