Crystallographic structure of the HindIII restriction endonuclease dimer (cyan and green) complexed with double helical DNA (brown) based on the PDB: 2E52 coordinates.
Identifiers
Symbol
RE_Hindiii
Pfam
PF09518
InterPro
IPR019043
Available protein structures:
Pfam
structures / ECOD
PDB
RCSB PDB; PDBe; PDBj
PDBsum
structure summary
hindIIIR type II restriction endonuclease
Identifiers
Symbol
hindIIIR
NCBI gene
950303
PDB
2e52 More structures
UniProt
P43870
Other data
EC number
3.1.21.4
Search for
Structures
Swiss-model
Domains
InterPro
Preview warning: Page using Template:Infobox protein with unknown parameter "Synonyms"
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.[1]
HindIII restrictions process results in formation of overhanging palindromic sticky ends.
The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends:
5'-A |A G C T T-3'
3'-T T C G A| A-5'
Restriction endonucleases are used as defense mechanisms in prokaryotic organisms in the restriction modification system. Their primary function is to protect the host genome against invasion by foreign DNA, primarily bacteriophage DNA. There is also evidence that suggests the restriction enzymes may act alongside modification enzymes as selfish elements, or may be involved in genetic recombination and transposition.[2]
^Tang, D; et al. (2000). "Mutational analyses of restriction endonuclease-HindIII mutant E86K with higher activity and altered specificity". Protein Engineering. 13 (4): 283–9. doi:10.1093/protein/13.4.283. PMID 10810160.
^Pingoud, Alfred; Jeltsch, Albert. (2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Research. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the...
by digesting with NcoI restriction enzyme and replacing the site with a HindIII linker. Although the whole construct is commonly referred to as the "CAG...
digests. Star activity can happen because of presence of Mg2+, as is seen in HindIII, for example. The term star activity was introduced by Mayer who characterized...
Technology portal BglII – A restriction enzyme EcoRI – A restriction enzyme HindIII – A restriction enzyme Homing endonuclease List of homing endonuclease...
lie within the TetR gene. There are two sites for restriction enzymes HindIII and ClaI within the promoter of the TetR gene. There are six key restriction...
endonucleases from H. influenzae strain d are named HindI, HindII, HindIII, etc. Another example: "HaeII" and "HaeIII" refer to bacterium Haemophilus aegyptius...
available pSC101 gained restriction sites for several enzymes, including HindIII, in addition to the EcoRI site. Manen D.; Caro L. (February 1991). "The...
pMB1. The lacZ gene codes for β-galactosidase. The recognition sites for HindIII, SphI, PstI, SalI, XbaI, BamHI, SmaI, KpnI, SacI and EcoRI restriction...
golden horns, golden-horned hind, Ceryneia hind, Cerynitian hind, beast with golden antlers, Parrhasian hind, nimble hind of Maenalus and beast of Maenalus...
Jason T. Hinds (born c. 1974) is a United States Air Force major general who serves as the director of operations of the United States European Command...
fragment and a single HindIII enzyme fragment. Clones carrying additional adjacent fragments were found to code for the HaeIII restriction enzyme. The...
spinocerebellar ataxia. PLD3 was first characterized as a human homolog of the HindIII K4L protein in the vaccinia virus, having a DNA sequence 48.1% similar...
enzymes (REs) that make cuts on 6bp recognition sequences, such as EcoR1 or HindIII, are used for this purpose, as they cut the genome once every 4000bp, giving...
1128/mcb.11.6.3296. PMC 360182. PMID 1710027. Ueyama H (May 1991). "A HindIII DNA polymorphism in the human enteric type smooth muscle actin gene (ACTSG)"...
which is a 1.7kb segment of the phage T12 genome flanked by SalI and HindIII sites. The phage integrase gene (int) and the phage attachment site (attp)...
1126/science.1692159. PMID 1692159. Kenney RT, Leto TL (December 1990). "A HindIII polymorphism in the human NCF2 gene". Nucleic Acids Research. 18 (23):...
William Henry Gates III (born October 28, 1955) is an American businessman, investor, philanthropist, and writer best known for co-founding the software...
Operation Mount Hope III was a top secret clandestine American military operation to capture a Soviet-made Mil Mi-25 "Hind-D" attack helicopter, an export...
Zaysen's Mi-24 Hind-D, culminating in a head-on charge as both unleash their vehicles' weaponry on each other; Rambo survives by destroying the Hind-D with his...
L.; Ward, D. C. (1984). "Chromosomal and nuclear distribution of the HindIII 1.9-kb human DNA repeat segment". Chromosoma. 91 (1): 28–38. doi:10.1007/BF00286482...
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