Restriction enzyme mediated integration information
Restriction enzyme mediated integration (abbreviated as REMI) is a technique for integrating DNA (linearised plasmid) into the genome sites that have been generated by the same restriction enzyme used for the DNA linearisation.[1] The plasmid integration occurs at the corresponding sites in the genome, often by regenerating the recognition sites by same the restriction enzyme used for plasmid linearisation.
^Schiestl, R. H.; Petes, T. D. (1991-09-01). "Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae". Proceedings of the National Academy of Sciences of the United States of America. 88 (17): 7585–7589. Bibcode:1991PNAS...88.7585S. doi:10.1073/pnas.88.17.7585. ISSN 0027-8424. PMC 52346. PMID 1881899.
and 24 Related for: Restriction enzyme mediated integration information
Restrictionenzymemediatedintegration (abbreviated as REMI) is a technique for integrating DNA (linearised plasmid) into the genome sites that have been...
A restrictionenzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition...
secreted in liquid cultures of Coprinopsis cinerea strains Restrictionenzyme-mediated DNA integration in Coprinus cinereus "Coprinopsis cinerea Genome Project"...
new proteins of biological interest. Examples include Restrictionenzymemediatedintegration, Gibson assembly and Golden Gate Cloning. DNA damages caused...
such as that borne by bacteriophages. Bacteria have restrictionenzymes, also called restriction endonucleases, which cleave double-stranded DNA at specific...
immunity. In practice, CRISPR/Cas systems act as self-programmable restrictionenzymes. CRISPR loci are composed of short, palindromic repeats that occur...
PMID 17922573. Liang KH, Yeh CT (May 2013). "A gene expression restriction network mediated by sense and antisense Alu sequences located on protein-coding...
high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of restrictionenzyme sites along the...
self and prevents DNA cleavage. RNA-guided CRISPR enzymes are classified as type V restrictionenzymes. The cas genes in the adaptor and effector modules...
APOBEC3G (apolipoprotein B mRNA editing enzyme, catalytic subunit 3G) is a human enzyme encoded by the APOBEC3G gene that belongs to the APOBEC superfamily...
last parts are mediated either by the aldosterone synthase (for aldosterone) or by the 11β-hydroxylase (for corticosterone). These enzymes are nearly identical...
Staphylococcal resistance to penicillin is mediated by penicillinase (a form of beta-lactamase) production: an enzyme that cleaves the β-lactam ring of the...
function and manipulation. Important advances included the discovery of restrictionenzymes, DNA ligases, and the development of polymerase chain reaction and...
across species) in yeast (Saccharomyces cerevisiae) is Sir2. SIRT1 is an enzyme located primarily in the cell nucleus that deacetylates transcription factors...
the RCA products are digested with a restrictionenzyme and ligated into new circular templates using a restriction oligo, followed by a new round of RCA...
sialylation (by the enzyme Lst) prevents phagocytosis by neutrophils and complement deposition. LOS modification by phosphoethanolamine (by the enzyme LptA) provides...
proteins by proteolysis, a chemical reaction that breaks peptide bonds. Enzymes that help such reactions are called proteases. Proteasomes are part of...
sensitive restrictionenzymes paired with PCR is a way to evaluate methylation in DNA - specifically the CpG sites. If DNA is methylated, the restriction enzymes...
reacts with an enzyme electrode containing glucose oxidase (or dehydrogenase). The enzyme is reoxidized with an excess of a mediator reagent, such as...
that are not being recognized by methylation-sensitive restrictionenzymes. The restrictionenzyme DpnI can recognize 5'-GmeATC-3' sites and digest the...
By comparing restriction fragment sizes generated from the methylation-sensitive enzyme to those of the methylation-insensitive enzyme, it was possible...
dismutase and ability to avoid complement mediated killing (fHbp). Meningococci produce an IgA protease, an enzyme that cleaves IgA class antibodies and thus...
to harmless antigens driven by a TH2-mediated immune response. Many bacteria and viruses elicit a TH1-mediated immune response, which down-regulates...
invading a host cell's cytoplasm, the virus uses its own reverse transcriptase enzyme to produce DNA from its RNA genome, the reverse of the usual pattern, thus...
Global Information