Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA.[1][2] These bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation–emission spectra.
^Latt, SA; Stetten, G; Juergens, LA; Willard, HF; Scher, CD (July 1975). "Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence". Journal of Histochemistry and Cytochemistry. 23 (7): 493–505. doi:10.1177/23.7.1095650. PMID 1095650.
^Latt, SA; Stetten, G (January 1976). "Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis". Journal of Histochemistry and Cytochemistry. 24 (1): 24–33. doi:10.1177/24.1.943439. PMID 943439.
Hoechststains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed by Hoechst AG, which numbered...
used with eosin in the H&E stain (haematoxylin and eosin) staining, one of the most common procedures in histology. Hoechst is a bis-benzimidazole derivative...
cells stainingHoechstStaining is used. DAPI gives a higher signal in the fixed cells compare to HoechstStain but in the live cells HoechstStain is used"...
Hoechst, Hochst, or Höchst may refer to: Hoechst AG, a former German life-sciences company Hoechststain, one of a family of fluorescent DNA-binding compounds...
Bisbenzimide (Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several related chemical compounds...
Crystal violet Methyl violet Nile blue Hoechststain "English Medical Dictionary". N. Chandler Foot (1954). "Vital staining". Annals of the New York Academy...
Verwaltungsgebäude) of Hoechst AG is an expressionist office building by the architect Peter Behrens on the site of the former Hoechst company in Frankfurt-Höchst...
(antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, such as...
molecule of interest. Major examples of these are nucleic acid stains such as DAPI and Hoechst (excited by UV wavelength light) and DRAQ5 and DRAQ7 (optimally...
(Mesenchymal stem cells). Recent studies have used hypoxic-staining dyes, such as Hoechststain, to show that quiescent LT-HSCs and osteoblasts are found...
phytoplasma by light microscopy Giemsa and Dienes’ stain are employed. Aniline blue, Hoechst 33258 and DAPI stain are used in the non-specific detection by fluorescence...
serum early in 1894 were successful and in August the chemical company Hoechst started to market Behring's "Diphtheria Remedy synthesized by Behring-Ehrlich"...
methodology do detect DNA replicating (S-phase) cells. Unless staining is performed using Hoechst 33342, the first step in preparing cells for cell cycle analysis...
mirror the different contents of AT. Other fluorochromes like DAPI or Hoechst 33258 lead also to characteristic, reproducible patterns. Each of them...
a staining agent for detecting collagen degradation and denaturation via immunofluorescence and immunohistochemistry applications. CHPs can stain frozen...
Merlin HR-SEM. HeLa cells, an example of an immortalised cell line. DIC image, DNA stained with Hoechst 33258. Anatomical terminology [edit on Wikidata]...
human with Alzheimer Disease. Sample was treated with Hoechst 33342 dye that is used to stain DNA. The picture shows the release of DNA by a neutrophil...
various (1978). Dictionary of Fiber & Textile Technology. Internet Archive. Hoechst Celanese. p. 16. "Learn How to Understand the Label on a Tube of Paint"...
system dysfunction. The nucleolus is the largest of the discrete densely stained, membraneless structures known as nuclear bodies found in the nucleus....
immunoassays, PCR, plating on sensitive agar and staining with a DNA stain including DAPI or Hoechst. An estimated 11 to 15% of U.S. laboratory cell cultures...
including eosin, rhodamine, DAPI, Hoechst, acridine orange, propidium iodide, and proflavine. Eosin and rhodamine stain the cytoplasm and the extracellular...
alternative starting materials at Badische Anilin- und Soda-Fabrik (BASF) and Hoechst continued. Johannes Pfleger and Karl Heumann eventually came up with industrial...
dye is imaged separately (for example, a blue filter is used to image Hoechst, then rapidly switched to a green filter to image GFP). For consumption...
various (1978). Dictionary of Fiber & Textile Technology. Internet Archive. Hoechst Celanese. Aspland, J. Richard (1997). Textile Dyeing and Coloration. AATCC...
characterized by their small size and low staining with vital dyes such as rhodamine 123 (rhodamine lo) or Hoechst 33342 (side population). The classical...
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