Functional domains in the Klenow Fragment (left) and DNA Polymerase I (right).
Identifiers
Organism
Escherichia coli (str. K-12 substr. MG1655)
Symbol
polA
Entrez
948356
PDB
1DPI
RefSeq (Prot)
NP_418300.1
UniProt
P00582
Other data
EC number
2.7.7.7
Chromosome
genome: 4.04 - 4.05 Mb
Search for
Structures
Swiss-model
Domains
InterPro
DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication. Discovered by Arthur Kornberg in 1956,[1] it was the first known DNA polymerase (and the first known of any kind of polymerase). It was initially characterized in E. coli and is ubiquitous in prokaryotes. In E. coli and many other bacteria, the gene that encodes Pol I is known as polA. The E. coli Pol I enzyme is composed of 928 amino acids, and is an example of a processive enzyme — it can sequentially catalyze multiple polymerisation steps without releasing the single-stranded template.[2] The physiological function of Pol I is mainly to support repair of damaged DNA, but it also contributes to connecting Okazaki fragments by deleting RNA primers and replacing the ribonucleotides with DNA.
^Lehman IR, Bessman MJ, Simms ES, Kornberg A (July 1958). "Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli". The Journal of Biological Chemistry. 233 (1): 163–70. doi:10.1016/S0021-9258(19)68048-8. PMID 13563462.
^Voet D, Voet JG, Pratt CW (1999). Fundamentals of Biochemistry. New York: Wiley.[page needed]
DNApolymeraseI (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication. Discovered by Arthur Kornberg in 1956, it was...
A DNApolymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors...
Taq polymerase is a thermostable DNApolymeraseI named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally...
DNApolymerase III holoenzyme is the primary enzyme complex involved in prokaryotic DNA replication. It was discovered by Thomas Kornberg (son of Arthur...
T7 DNApolymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNApolymerase “reads” existing DNA strands...
DNApolymerase epsilon is a member of the DNApolymerase family of enzymes found in eukaryotes. It is composed of the following four subunits: POLE (central...
DNApolymerase (DNA-directed DNApolymerase, DdDP) Family A: DNApolymeraseI; Pol γ, θ, ν Family B: DNApolymerase II; Pol α, δ, ε, ζ Family C: DNA polymerase...
In molecular biology, RNA polymerase (abbreviated RNAP or RNApol), or more specifically DNA-directed/dependent RNA polymerase (DdRP), is an enzyme that...
when DNApolymeraseI from E. coli is enzymatically cleaved by the protease subtilisin. First reported in 1970, it retains the 5' → 3' polymerase activity...
DNApolymerase beta, also known as POLB, is an enzyme present in eukaryotes. In humans, it is encoded by the POLB gene. In eukaryotic cells, DNA polymerase...
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...
helicase, RFC, PCNA, gyrase/topoisomerase, SSB/RPA, primase, DNApolymerase III, RNAse H, and DNA ligase. For prokaryotes, each dividing nucleoid (region containing...
in which DNAPolymeraseI is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which...
study the mechanism of DNA replication. By 1957 he has identified the first DNApolymerase. The enzyme was limited, creating DNA in just one direction...
genome. DNA replication is the action of DNApolymerases synthesizing a DNA strand complementary to the original template strand. To synthesize DNA, the...
transcribed by RNA Polymerase III. "gDNA - Definitions from Dictionary.com". dictionary.reference.com. Retrieved 2008-04-26. "What is Genomic DNA? (with pictures)"...
synthesized partner strand. DNApolymerases are a family of enzymes that carry out all forms of DNA replication. DNApolymerases in general cannot initiate...
the polymerase. This process involves the exonuclease's catching up to the pol II and terminating the transcription. Pol I then synthesizes DNA nucleotides...
DNApolymerase lambda, also known as Pol λ, is an enzyme found in all eukaryotes. In humans, it is encoded by the POLL gene. Pol λ is a member of the X...
and DNAPolymeraseI (exonuclease), and the gaps (or nicks) are filled with deoxyribonucleotides and sealed by the enzyme ligase. The rate of DNA replication...
added by a polymerase enzyme, such as DNApolymerase, per association event with the template strand. Because the binding of the polymerase to the template...
Coli RNase H to nick mRNA that is replaced with E. Coli DNAPolymeraseI and sealed with E. Coli DNA Ligase. An optimization of this procedure relies on low...
the replication of DNA, for example, the DNApolymeraseI leaves behind a hole between the phosphates in the newly formed backbone. DNA ligase is able to...
are ultimately replaced with DNA bases by RNase H nuclease (eukaryotes) or DNApolymeraseI nuclease (prokaryotes). DNA Ligase then acts to join the two...
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