DNA end resection, also called 5′–3′ degradation, is a biochemical process where the blunt end of a section of double-stranded DNA (dsDNA) is modified by cutting away some nucleotides from the 5' end to produce a 3' single-stranded sequence.[1][2] The presence of a section of single-stranded DNA (ssDNA) allows the broken end of the DNA to line up accurately with a matching sequence, so that it can be accurately repaired.[1]
Mechanism of regulation of 5' resection of Mitotic and Telomeric DSBs.[3]
Double-strand breaks (DSBs) can occur at any phase of the cell cycle causing DNA end resection and repair activities to take place, but they are also normal intermediates in mitosis recombination.[3] Furthermore, the natural ends of the linear chromosomes resemble DSBs, and although DNA breaks can cause damage to the integrity of genomic DNA, the natural ends are packed into complex specialized DNA protective packages called telomeres that prevent DNA repair activities.[3][4] Telomeres and mitotic DSBs have different functionality, but both experience the same 5′–3′ degradation process.
^ abJimeno S, Mejías-Navarro F, Prados-Carvajal R, Huertas P (2019). "Controlling the balance between chromosome break repair pathways". Advances in Protein Chemistry and Structural Biology. 115. Elsevier: 95–134. doi:10.1016/bs.apcsb.2018.10.004. ISBN 9780128155592. PMID 30798939. S2CID 73459973.
^ abcLonghese MP, Bonetti D, Manfrini N, Clerici M (September 2010). "Mechanisms and regulation of DNA end resection". The EMBO Journal. 29 (17): 2864–2874. doi:10.1038/emboj.2010.165. PMC 2944052. PMID 20647996.
^Mimitou EP, Symington LS (September 2009). "DNA end resection: many nucleases make light work". DNA Repair. 8 (9): 983–995. doi:10.1016/j.dnarep.2009.04.017. PMC 2760233. PMID 19473888.
DNAendresection, also called 5′–3′ degradation, is a biochemical process where the blunt end of a section of double-stranded DNA (dsDNA) is modified...
distances to known points DNAendresection, the process of cutting away the 5′ side of a blunt end of double-stranded DNA This disambiguation page lists...
vivo and may serve to tether broken ends prior to repair by non-homologous end joining or to initiate DNAendresection prior to repair by homologous recombination...
cooperates with the WRN and BLM RecQ helicases to mediate long-range DNAendresection in human cells". J. Biol. Chem. 289 (39): 27314–26. doi:10.1074/jbc...
called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then "invades" a similar or identical DNA molecule...
Jackson, Stephen P. (2010-09-10). "RETRACTED: Human SIRT6 promotes DNAendresection through CtIP deacetylation". Science. 329 (5997): 1348–1353. Bibcode:2010Sci...
cooperates with the WRN and BLM RecQ helicases to mediate long-range DNAendresection in human cells". J. Biol. Chem. 289 (39): 27314–26. doi:10.1074/jbc...
recruitment of 53BP1 in G1 cells directs DNA double-strand break repair to BRCA1/CtIP-mediated DNA-endresection". Aging. 12 (24): 24872–24893. doi:10.18632/aging...
2013). "Microhomology-mediated End Joining and Homologous Recombination share the initial endresection step to repair DNA double-strand breaks in mammalian...
Jackson, Stephen P. (2010-09-10). "RETRACTED: Human SIRT6 promotes DNAendresection through CtIP deacetylation". Science. 329 (5997): 1348–1353. Bibcode:2010Sci...
scientific contributions include the structural and molecular mechanism of DNAendresection by RecBCD (E. coli) and DNA2-Sgs1-RPA and regulatory stimulation by...
referred to as endresection. When RECQL4 is deficient, endresection, and thus HR, is reduced. Evidence suggests that other forms of DNA repair including...
tumor DNA (ctDNA) is tumor-derived fragmented DNA in the bloodstream that is not associated with cells. ctDNA should not be confused with cell-free DNA (cfDNA)...
component of the nucleosome remodeling and deacetylase complex NuRD. DNAendresection is a pivotal step in HRR repair that produces 3’ overhangs that provide...
of the double-strand break to yield 3’ single-stranded DNA tails (a process called endresection). Next the Rad51 protein binds to these tails and initiates...
patients (44) (45) (46). Identification of new factors inhibiting DNAendresection, REV7 (47) and HELB (48), contributing to drug resistance in a mouse...
techniques, including endoscopic mucosal resection or endoscopic submucosal dissection. Endoscopic resection is possible if there is low possibility of...
Hollingsworth, Nancy M.; Haber, James E. (October 2004). "DNAendresection, homologous recombination and DNA damage checkpoint activation require CDK1". Nature...
PMID 31495888. Mimitou EP, Symington LS (September 2009). "DNAendresection: many nucleases make light work". DNA Repair. 8 (9): 983–995. doi:10.1016/j.dnarep.2009...
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