Cell cycle regulated Methyltransferase information
Bacterial enzyme
The X-ray crystal structure Caulobacter crescentus CcrM showing the CcrM homodimer in complex with double strand DNA. The protein monomers are shown in blue and green while the DNA is shown in tan. The DNA Bases in the GANTC recognition site are shown in all atom resolution.
CcrM (or M.CcrMI) is an orphan DNA methyltransferase, that is involved in controlling gene expression in most Alphaproteobacteria. This enzyme modifies DNA by catalyzing the transference of a methyl group from the S-adenosyl-L methionine substrate to the N6 position of an adenine base in the sequence 5'-GANTC-3' with high specificity.[1] In some lineages such as SAR11, the homologous enzymes possess 5'-GAWTC-3' specificity.[2] In Caulobacter crescentus Ccrm is produced at the end of the replication cycle when Ccrm recognition sites are hemimethylated, rapidly methylating the DNA. CcrM is essential in other Alphaproteobacteria but its role is not yet determined. CcrM is a highly specific methyltransferase with a novel DNA recognition mechanism.[3]
^Reich, Norbert O.; Dang, Eric; Kurnik, Martin; Pathuri, Sarath; Woodcock, Clayton B. (2018-12-07). "The highly specific, cell cycle–regulated methyltransferase from Caulobacter crescentus relies on a novel DNA recognition mechanism". Journal of Biological Chemistry. 293 (49): 19038–19046. doi:10.1074/jbc.RA118.005212. ISSN 0021-9258. PMC 6295719. PMID 30323065.
Woodcock, Clayton B. (2018-12-07). "The highly specific, cellcycle–regulatedmethyltransferase from Caulobacter crescentus relies on a novel DNA recognition...
Methyltransferases are a large group of enzymes that all methylate their substrates but can be split into several subclasses based on their structural...
in a 2018 Phase III AML trial. Methyltransferase DNA methylation PRMT4 pathway CellcycleregulatedMethyltransferase Iyer LM, Zhang D, Aravind L (January...
is not the only example of an orphan methyltransferase as there exists the CellcycleregulatedMethyltransferase (CcrM) which methylates 5'-GANTC-'3 hemi-methylated...
differentiation, a precursor cell formerly capable of cell division permanently leaves the cellcycle, dismantles the cellcycle machinery and often expresses...
diagnostics and staging. Life cycle of a cancer cell Cancer cells are created when the genes responsible for regulatingcell division are damaged. Carcinogenesis...
cardiac competent cells when treated with G9a histone methyltransferase inhibitor BIX01294. Cell plasticity, which is the adaptation of cells to stimuli without...
pRb is to prevent excessive cell growth by inhibiting cellcycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is...
(December 2010). "Phosphorylation of the PRC2 component Ezh2 is cellcycle-regulated and up-regulates its binding to ncRNA". Genes & Development. 24 (23): 2615–2620...
regulation of gene expression, arginine methyltransferases control the cellcycle and death of eukaryotic cells. While all PRMT enzymes catalyze the methylation...
trapped DNA methyltransferases. As a consequence, methylation marks become lost during DNA replication. Azacitidine causes anemia (low red blood cell counts)...
DNA (cytosine-5)-methyltransferase 1 (Dnmt1) is an enzyme that catalyzes the transfer of methyl groups to specific CpG sites in DNA, a process called...
critical for cell fate determination in the developing and adult mammalian brain. DNA cytosine methylation is catalyzed by DNA methyltransferases (DNMTs)....
O-methyltransferase (HIOMT), also known as acetylserotonin O-methyltransferase (ASMT). Activity of these enzymes is high during the night and regulated...
Histone-lysine N-methyltransferase 2A, also known as acute lymphoblastic leukemia 1 (ALL-1), myeloid/lymphoid or mixed-lineage leukemia 1 (MLL1), or zinc...
replication to once per cellcycle. Expression of certain genes, for example, those coding for pilus expression in E. coli, is regulated by the methylation...
by which a cellregulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range...
rapidly inactivated by its primary degradative enzymes, histamine-N-methyltransferase or diamine oxidase. In the central nervous system, histamine released...
Masumoto H, Hawke D, Kobayashi R, Verreault A (July 2005). "A role for cell-cycle-regulated histone H3 lysine 56 acetylation in the DNA damage response". Nature...
networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, cell proliferation, cell differentiation, cell adhesion, apoptosis...
trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1". Genes & Development...
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