The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids, phosphates, and glycoconjugates. It is most often used to detect carbohydrate epitopes. Thus, eastern blot can be considered an extension of the biochemical technique of western blot. Multiple techniques have been described by the term "eastern blot(ting)", most use phosphoprotein blotted from sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel on to a polyvinylidene fluoride or nitrocellulose membrane. Transferred proteins are analyzed for post-translational modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. Eastern blotting should be used to refer to methods that detect their targets through specific interaction of the post-translational modifications and the probe, distinguishing them from a standard far-western blot. In principle, eastern blotting is similar to lectin blotting (i.e., detection of carbohydrate epitopes on proteins or lipids).[1]
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The easternblot, or easternblotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids...
first published it in 1975. Other blotting methods (i.e., western blot, northern blot, easternblot, southwestern blot) that employ similar principles,...
The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a...
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics...
northern, western and easternblotting are derived from what initially was a molecular biology joke that played on the term Southern blotting, after the technique...
matrix. Southern blot Western blot Northern blot Southwestern blotEasternblot Gel electrophoresis SDS-PAGE Chromatography Northwestern Blot of Protein-RNA...
extensively used experimental tool. The northern blot, western blot and easternblot, related procedures for the analysis of RNA, protein and post-translational...
associated with glycoproteins. Proteins can be blotted from CTAB-gels in analogy to western blots ("easternblot"), and Myelin-associated high hydrophobic...
Runic Mesoamerican Long Count Calendar round Christian variants Coptic Ethiopian and Eritrean Julian Revised Liturgical year Eastern Orthodox Saints...
gel electrophoresis Capillary electrophoresis DNA electrophoresis Easternblotting Electroblotting Fast parallel proteolysis (FASTpp) History of electrophoresis...
religious practices, being one of the most common animals sacrificed in blóts and found in graves, notably in examples such as at Sutton Hoo and the Oseberg...
having been repealed, all time may be dumb concerning him ; and that the blot of our age may not appear by the mention of him ; and that those who by their...
board. Modern backgammon follows the same rules as tabula for hitting a blot and for bearing off; and the rules for re-entering pieces in backgammon are...
WB" or "the West" Weber (unit) (Wb), the SI unit of magnetic flux Western blot, a medical diagnosis tool White balance, a technical term in color photography...
This article will attempt to outline the common traits of ancient Near Eastern religions, and refer to sub-articles for in-depth descriptions. The ancient...
higher Aqua Mahjong AquaMan Doug Diego 2002 Arcade Freeware 10 Aquaria Bit Blot 2008 Action-adventure Commercial 10.6 Aquataxx Aquazone 9003 Inc. 1993 Simulation...
this custom ceased, the squadrons of soldiers and the boundary wall were blotted out together. The troop at Batavis, however, held out. Some soldiers of...
of a prophecy of the city's demise. Four days later, the whole city was blotted out by a thick fog, a condition unknown in that part of the world in May...
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