A thrombin generation assay (TGA) or thrombin generation test (TGT) is a global coagulation assay (GCA) and type of coagulation test which can be used to assess coagulation and thrombotic risk.[1][2][3] It is based on the potential of a plasma to generate thrombin over time, following activation of coagulation via addition of phospholipids, tissue factor, and calcium.[4] The results of the TGA can be output as a thrombogram or thrombin generation curve using computer software with calculation of thrombogram parameters.[5][1]
TGAs can be performed with methods like the semi-automated calibrated automated thrombogram (CAT) (2003) or the fully-automated ST Genesia system (2018).[6][1][7] TGAs were first used as manual assays in the 1950s and have since become increasingly automated.[1]
^ abcdDepasse F, Binder NB, Mueller J, Wissel T, Schwers S, Germer M, Hermes B, Turecek PL (December 2021). "Thrombin generation assays are versatile tools in blood coagulation analysis: A review of technical features, and applications from research to laboratory routine". J Thromb Haemost. 19 (12): 2907–2917. doi:10.1111/jth.15529. PMC 9291770. PMID 34525255. S2CID 237535808.
^Duarte RC, Ferreira CN, Rios DR, Reis HJ, Carvalho MD (2017). "Thrombin generation assays for global evaluation of the hemostatic system: perspectives and limitations". Rev Bras Hematol Hemoter. 39 (3): 259–265. doi:10.1016/j.bjhh.2017.03.009. PMC 5568585. PMID 28830606.
^Morimont L, Haguet H, Dogné JM, Gaspard U, Douxfils J (2021). "Combined Oral Contraceptives and Venous Thromboembolism: Review and Perspective to Mitigate the Risk". Front Endocrinol (Lausanne). 12: 769187. doi:10.3389/fendo.2021.769187. PMC 8697849. PMID 34956081.
^Tripodi A (May 2016). "Thrombin Generation Assay and Its Application in the Clinical Laboratory". Clin Chem. 62 (5): 699–707. doi:10.1373/clinchem.2015.248625. PMID 26955824. S2CID 207378147.
^Reda S, Morimont L, Douxfils J, Rühl H (August 2020). "Can We Measure the Individual Prothrombotic or Prohemorrhagic Tendency by Global Coagulation Tests?". Hamostaseologie. 40 (3): 364–378. doi:10.1055/a-1153-5824. PMID 32726831. S2CID 220878363.
^Kintigh J, Monagle P, Ignjatovic V (January 2018). "A review of commercially available thrombin generation assays". Res Pract Thromb Haemost. 2 (1): 42–48. doi:10.1002/rth2.12048. PMC 6055498. PMID 30046705.
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Fibrin glue Fibrinogen PA clan of proteases The Proteolysis Map Thrombingenerationassay GRCh38: Ensembl release 89: ENSG00000180210 – Ensembl, May 2017...
calibrated automated thrombogram (CAT or CT) is a thrombingenerationassay (TGA) and global coagulation assay (GCA) which can be used as a coagulation test...
to a thrombingenerationassay (TGA). This results in an inhibition of thrombingeneration as measured by reduction of the endogenous thrombin potential...
global coagulation assay which can be used to measure coagulation. The OHP assay measures total fibrin generation in the presence of thrombin or tissue factor...
prothrombin ratio (PR) and international normalized ratio (INR) – is an assay for evaluating the extrinsic pathway and common pathway of coagulation....
thrombin clotting time (TCT). Measurement of the exact amount of fibrinogen present in the blood is generally done using the Clauss fibrinogen assay....
activated partial thromboplastin times are related to increased thrombingeneration and an increased risk for thromboembolism". American Journal of Clinical...
Reptilase time Ecarin clotting time Dilute Russell's viper venom time Thrombingenerationassay Calibrated automated thrombogram ST Genesia-based test Thromboelastography...
with thrombin, and thus slow down clot formation by preventing the conversion of fibrinogen to fibrin. This effect can be seen in the thrombin clotting...
"Dabigatran etexilate – a novel, reversible, oral direct thrombin inhibitor: Interpretation of coagulation assays and reversal of anticoagulant activity". Thrombosis...
is a fully automated commercial analyzer system for performing thrombingenerationassays (TGAs) and hence for coagulation testing. It was developed by...
research and medical tests. If multiple aptamers are combined into a single assay, they can measure large numbers of different proteins in a sample. They...
PT (prothrombin time), aPTT (activated partial thromboplastin time) or thrombin time are not very sensitive for hyperfibrinolysis, and influenced by numerous...
disulfide bond.: 4673 The protein C zymogen is activated when it binds to thrombin, another protein heavily involved in coagulation, and protein C's activation...
receptors, for example in the biosynthesis of cholesterol, or the mediation of thrombin signalling through protease-activated receptors. Some enzymes at important...
When factor V remains active, it facilitates overproduction of thrombin leading to generation of excess fibrin and excess clotting. The excessive clotting...
Factor Va and/or Factor VIIIa, which allows for longer duration of thrombingeneration and may lead to a hypercoagulable state. This may be hereditary or...
nonetheless possibly influence vascular function, with some upregulation of the thrombin receptor observed with etonogestrel in vascular smooth muscle cells in...
specific chemical reaction Anti-thrombin aptamers – Oligonucleotides which recognize the exosites of human thrombin Bacterial one-hybrid system – Method...
intravascular coagulation (DIC): Involving abnormal, excessive generation of thrombin and fibrin within the blood. Relative to hypoprothrombinemia, due...
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