A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. The history of suspension cell culture closely aligns with the history of cell culture overall, but differs in maintenance methods and commercial applications. The cells themselves can either be derived from homogenized tissue or from heterogenous cell solutions. Suspension cell culture is commonly used to culture nonadhesive cell lines like hematopoietic cells, plant cells, and insect cells.[1] While some cell lines are cultured in suspension, the majority of commercially available mammalian cell lines are adherent.[2][3] Suspension cell cultures must be agitated to maintain cells in suspension, and may require specialized equipment (e.g. magnetic stir plate, orbital shakers, incubators) and flasks (e.g. culture flasks, spinner flasks, shaker flasks).[4] These cultures need to be maintained with nutrient containing media and cultured in a specific cell density range to avoid cell death.[5]
A cell suspension or suspensionculture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply...
refer to: Car suspension Cell suspension or suspension culture, in biology Guarded suspension, a software design pattern in concurrent programming suspending...
an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspensionculture. This is typically...
proteases could damage the cell cultures. Unlike suspensioncultures, the other main type of cell culture, adherent cultures require regular passaging performed...
have been a cell line of choice because of their rapid growth in suspensionculture and high protein production. The thrombolytic medication against myocardial...
patch clamping cells in suspensioncultures. One system uses a traditional pipette and cells in a droplet suspensionculture to obtain patch clamp recordings...
Antifeedant Activity in a Two-Stage Bioreactor Process with Cell SuspensionCulture of Azadirachta indica". Applied Biochemistry and Biotechnology. 177...
monolayer cultures, however, the spheroid structures that are formed when ESCs aggregate enables the non-adherent culture of EBs in suspension, making EB...
Suspension of disbelief is the avoidance—often described as willing—of critical thinking and logic in understanding something that is unreal or impossible...
epidermal cells of roots, hypocotyls, and petals, and in callus and suspensionculture cells of tobacco. In some cell types at certain stages of development...
Air suspension is a type of vehicle suspension powered by an electric or engine-driven air pump or compressor. This compressor pumps the air into a flexible...
Suspension bondage is a form of sexual bondage where a bound person is hung from one or more overhead suspension points. It carries a higher risk than...
sterile solid culture medium but are sometimes placed directly into a sterile liquid medium, particularly when cell suspensioncultures are desired. Solid...
lymphoblastoid cells in suspensioncultures, but can also support a wide variety of adherent cells. It was originally developed to culture human leukemic cells...
export signal in a protein HEK 293 cells were adapted to grow in suspensionculture, as opposed to proliferation on plastic plates, in 1985. This enabled...
(2014). "SuspensionCulture of Plant Cells under Heterotrophic Conditions". In Meyer HP, Schmidhalter D (eds.). Industrial Scale SuspensionCulture of Living...
was by Steward et al. in 1958 and Reinert in 1959 with carrot cell suspensioncultures. Somatic embryogenesis has been described to occur in two ways: directly...
cell formation and physiology. HL-60 proliferates continuously in suspensionculture in nutrient and antibiotic chemicals. The doubling time is about 36–48...
cells markers such as CD90, CD105. When subjected to single cell suspensionculture, the cells will generate clusters that are similar to embryoid bodies...
Mexican-American youth culture. Conversion of standard production vehicles included adding lowering blocks and cut-down spindles, reduced-length suspension spring coils...
The Capilano Suspension Bridge is a simple suspension bridge crossing the Capilano River in Upper Capilano, British Columbia, Canada, in the District of...
A simple suspension bridge (also rope bridge, swing bridge (in New Zealand), suspended bridge, hanging bridge and catenary bridge) is a primitive type...
standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range to standardize...
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