Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur.[1] Most vertebrate derived cells (with the exception of hematopoietic cells) can be cultured and require a 2 dimensional monolayer that to facilitate cell adhesion and spreading.[2] Cell samples can be taken from tissue explants or cell suspension cultures. Adherent cell cultures with an excess of nutrient-containing growth medium will continue to grow until they cover the available surface area.[3] Proteases like trypsin are most commonly used to break the adhesion from the cells to the flask. Alternatively, cell scrapers can be used to mechanically break the adhesion if introducing proteases could damage the cell cultures.[4][5] Unlike suspension cultures, the other main type of cell culture, adherent cultures require regular passaging performed using mechanical or enzymatic dissociation.[1] The culture can be visualized using an inverted microscope, however the growth of adherent cultures is dependent on the available surface area. For this reason, adherent cell cultures are not commonly used to obtain a high yield of cells, instead the use of suspension cultures is preferred.[4]
Capsaspora owczarzaki adherent cells visualized using fluorescence microscopy.
Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur. Most vertebrate derived...
Suspension culture is one of the two classical types of cell culture, the other being adherentculture. The history of suspension cell culture closely aligns...
form an adherentculture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. This is...
disinformation actors insert polarizing claims in culture wars by taking one side or the other, thus making the adherents circulate and parrot disinformation as...
art and culture which are influenced by their faith and synthesize traditions from many other cultures depending on the locality of the adherents of the...
of laïcité, which relies on the division between private life, where adherents believe religion belongs, and the public sphere, in which each individual...
Tamil culture is the culture of the Tamil people. The Tamil people speak the Tamil language, one of the ancient languages in the world. Archaeological...
Northeastern Neo-Aramaic and Central Neo-Aramaic. They are predominantly adherents of several denominations of Syriac Christianity, notably the Ancient Church...
Chinese culture (simplified Chinese: 中华文化; traditional Chinese: 中華文化; pinyin: Zhōnghuá wénhuà) is one of the world's oldest cultures, originating thousands...
Minangkabau culture is the culture of the Minangkabau ethnic group in Indonesia, part of the Indonesian culture. This culture is one of the two major cultures in...
cultures with stronger 'extended family traditions', such as Asian and Catholic countries Major Branches of Religions Ranked by Number of Adherents Thomas...
contrast to monolayer cultures, however, the spheroid structures that are formed when ESCs aggregate enables the non-adherentculture of EBs in suspension...
mainly inhabit the highlands. Religious denominations with fewer than 5% adherents include Protestants, Seventh-day Adventists, Jehovah's Witnesses, Baha'is...
Indian culture is the heritage of social norms and technologies that originated in or are associated with the ethno-linguistically diverse India, pertaining...
legal status for the use of LSD and other psychedelics for the religion's adherents based on a "freedom of religion" argument. The Psychedelic Experience...
The traditional culture of Korea is the shared cultural and historical heritage of Korea before the division of Korea in 1945. Since the mid-20th century...
Islamic culture or Muslim culture refers to the historic cultural practices that developed among the various peoples living in the Muslim world. These...
scale for patterning, and the use of culture conditions derived from directed differentiation of ESCs in adherentculture Brachyury (T/Bra), a gene which marks...
The culture of Nigeria is shaped by Nigeria's multiple ethnic groups. The country has 527 languages, seven of which are extinct. Nigeria also has over...
The Ethical movement (also the Ethical Culture movement, Ethical Humanism, and Ethical Culture) is an ethical, educational, and religious movement established...
came from the Galilean village of Nazareth, today in northern Israel. Adherents of Messianic Judaism are referred to in modern Hebrew as יְהוּדִים מְשִׁיחִיִּים...
Roman Catholic Church with 249,619 adherents in 2010; the Church of Jesus Christ of Latter-day Saints with 68,128 adherents in 2009; the United Church of Christ...
religious festivals include the Xooy, Mbosseh, and Randou Rande. Each year, adherents of Serer religion make the annual pilgrimage to Sine in Senegal for the...
with the Avestan word yasna, meaning "worship, devotion". In English, an adherent of the faith is commonly called a Zoroastrian or a Zarathustrian. An older...
of one culture or identity by members of another culture or identity. This can be especially controversial when members of a dominant culture appropriate...
Philippines in 200–300 AD while Vajrayana Buddhism arrived around 900 AD. Most adherent of Hinduism have Indian origins while those practicing Buddhism have Chinese...
weakness of being in perpetual diaspora away from Israel. Islam, whose adherents had been treated as overtly "feminine" during the colonial era, came to...
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