screening and diagnosis of paroxysmal nocturnal hemoglobinuria
The sucrose lysis test is a diagnostic laboratory test used for diagnosing paroxysmal nocturnal hemoglobinuria (PNH), as well as for hypoplastic anemias and any hemolytic anemia with an unclear cause.[1] The test works by using sucrose, which creates a low ionic strength environment that allows complement to bind to red blood cells.[1] In individuals with PNH, some red blood cells are especially vulnerable to lysis caused by complement.[1] The test may also produce suspicious results in other hematologic conditions, including megaloblastic anemia and autoimmune hemolytic anemia.[1] False-negative results can occur when complement activity is absent in the serum.[1] A simpler alternative called the sugar water test also involves mixing blood with sugar and observing for hemolysis, using the same principle as the sucrose lysis test.[1]
^ abcdefMcPherson, Richard A.; Pincus, Matthew R.; Henry, John Bernard (2007). Henry's clinical diagnosis and management by laboratory methods. Philadelphia: Saunders Elsevier. ISBN 978-1-4160-0287-1. OCLC 70327781.
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The sucroselysistest is a diagnostic laboratory test used for diagnosing paroxysmal nocturnal hemoglobinuria (PNH), as well as for hypoplastic anemias...
blood cell production in addition to the hemolysis. Historically, the sucroselysistest, in which a patient's red blood cells are placed in low-ionic-strength...
organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony....
increase blood uric acid levels by interfering with renal clearance. Tumor lysis syndrome, a metabolic complication of certain cancers or chemotherapy, due...
ability to be lysed by sodium deoxycholate. Lysis indicates S. Pneumoniae while no lysis does not. A CAMP test is used to differentiate between Streptococcus...
the sucrose and pH levels had minor effects, as well. Research has also been done on the response of P. multocida to the host environment. These tests use...
purification of a given particle. Sucrose gradient centrifugation—a linear concentration gradient of sugar (typically sucrose, glycerol, or a silica based...
thought to serve as a protective mechanism against UV irradiation, enzymatic lysis, oxidant attack, and fungal infections from other competing fungi. A method...
pathways and are not involved in cell wall lysis. For example, Invertase catalyzes the conversion of sucrose (common table sugar) into glucose and fructose...
bacteria is reduced when simple sugars like glucose, fructose as well as sucrose, honey and orange juice were ingested, while the ingestion of starches...
trimethylammonium bromide (CTAB). Detergents are key reagents to extract protein by lysis of the cells and tissues: They disorganize the membrane's lipid bilayer...
this process, a bacteriophage must first infect the donor cell so that lysis of the cell will occur. At this point, the cell lysate will be filled with...
other six-carbon sugars (also, disaccharides of six-carbon sugars, e.g. sucrose or lactose) are converted into cellular energy and the metabolite lactate...
ExoU, which degrades the plasma membrane of eukaryotic cells, leading to lysis. Increasingly, it is becoming recognized that the iron-acquiring siderophore...
Moreover, it was found that CB has an inhibitory effect on the uptake of sucrose-3H by chang-strain human liver cells and in CB-treated cells alterations...
while most of the original grape must resources have been consumed, the lysis of dead yeast cells (the "lees") can be a source for some nutrients, particularly...
NADPH and releases three-carbon sugars, which are later combined to form sucrose and starch. The overall equation for the light-independent reactions in...
away from other cellular components. This process usually begins with cell lysis, in which a cell's membrane is disrupted and its internal contents released...
fish extract) originating from Phú Quốc. The extract is produced through lysis, hydrolysis and self-fermentation of fish flesh by enzymes, most of which...
break. This can decrease the rate of soil respiration temporarily, but the lysis of bacteria causes for a spike in resources for many other bacteria. This...
infects humans. Phage particles are secreted from bacterial cells without lysis. When CTXφ infects V. cholerae cells, it integrates into specific sites...
activates NK is HN. HN protein binding to NKp46 and/or NKp44 results in the lysis of cells whose surfaces display the HN protein or its fragments. It can...