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Sporulation in Bacillus subtilis information


Figure Caption: The Bacillus subtilis (Stage I) in vegetative growth is in unfavorable conditions, so it begins the process of sporulation. Examples of unfavorable conditions are an environment that lacks the required nutrients, is too hot, or too cold. Asymmetric (unequal) division occurs from the tightening of the Z-ring (Stage II). The Z-ring is multiple FtsZ proteins assembled into a ring that depolymerizes to cause an inward constriction, which will form the septum that results in two daughter cells. The mother cell, which is the bigger of the two daughter cells, engulfs the pre-spore (Stage III). Next, the cortex (Stage IV) and the coat (Stage V) form around the spore. The cortex is made of peptidoglycan and the coat is composed of several layers of specific proteins. Once the spore is mature, the cell lyses (Stage VI and VII). Thus, a free endospore is formed that can withstand harsh environments. This endospore can later germinate into a vegetative cell. Vegetative cycle occurs in favorable conditions, such as nutrient abundance and a moderate temperature. This is a cycle of medial division and growth creating more vegetative Bacillus subtilis.
Fig1. The sporulation process of Bacillus subtilis

Bacillus subtilis is a rod-shaped, Gram-positive bacteria that is naturally found in soil and vegetation, and is known for its ability to form a small, tough, protective and metabolically dormant endospore. B. subtilis can divide symmetrically to make two daughter cells (binary fission), or asymmetrically, producing a single endospore that is resistant to environmental factors such as heat, desiccation, radiation and chemical insult which can persist in the environment for long periods of time. The endospore is formed at times of nutritional stress, allowing the organism to persist in the environment until conditions become favourable. The process of endospore formation has profound morphological and physiological consequences: radical post-replicative remodelling of two progeny cells, accompanied eventually by cessation of metabolic activity in one daughter cell (the spore) and death by lysis of the other (the ‘mother cell’).

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Sporulation in Bacillus subtilis

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SubtiList - GenoList - Institut Pasteur SubtilisWiki (archived) Bacillus subtilis - Parts Registry Video - Sporulation in Bacillus subtilis Portal: Biology...

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Bacillus subtilis

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Bacillus subtilis(/bəˈsɪl.əs subˈtiː.lis/), known also as the hay bacillus or grass bacillus, is a gram-positive, catalase-positive bacterium, found in...

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Bacillus megaterium

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subtilis for this purpose, B. megaterium was the main model organism among Gram-positive bacteria for intensive studies on biochemistry, sporulation,...

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Bacillus atrophaeus

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of sporulation. "Species: Bacillus atrophaeus". lpsn.dsmz.de. Nakamura, L. K. (1989). "Taxonomic Relationship of Black-pigmented Bacillus subtilis Strains...

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Endospore

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endospores were first hypothesized by Ferdinand Cohn after studying Bacillus subtilis growth on cheese after boiling the cheese. His notion of spores being...

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Flavoprotein

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the Genes Encoding Dipicolinic Acid Synthetase Required for Sporulation in Bacillus subtilis". Journal of Molecular Biology. 232 (2): 468–83. doi:10.1006/jmbi...

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Autolysin

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the involvement of two compensatory autolysins in mother cell lysis during sporulation of Bacillus subtilis 168". Journal of Bacteriology. 177 (13): 3855–3862...

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James Hoch

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dissecting the signal transduction mechanism that controls sporulation in Bacillus subtilis. He introduced the widely used phrase 'phosphorelay' to describe...

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Autoinducer

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adapted for dispersal and survival in unfavorable conditions. Sporulation in B. subtilis is mediated by CSF (sporulation factor), a pentapeptide cleaved...

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Peribacillus

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and Ectobacillus. In addition, the genus was restricted to only include species closely related to Bacillus subtilis and Bacillus cereus. The name Peribacillus...

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Sda protein domain

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inhibitor of the histidine kinases that regulate initiation of sporulation in Bacillus subtilis". Mol Cell. 13 (5): 689–701. doi:10.1016/S1097-2765(04)00084-X...

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Alan Grossman

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normal chromosome segregation as well as the initiation of sporulation in Bacillus subtilis". J Bacteriol. 176 (17): 5320–9. doi:10.1128/jb.176.17.5320-5329...

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SpoIVB peptidase

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of SpoIVB This enzyme participates in gene expression during the later stages of spore formation in Bacillus subtilis. Wakeley PR, Dorazi R, Hoa NT, Bowyer...

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Laetiporus

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(Staphylococcus aureus), as well as moderate ability to inhibit the growth of Bacillus subtilis. List of meat substitutes Lindner, D.L.; Banik, M.T. (2008). "Molecular...

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Network motif

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transcription for a single differentiating cell type during sporulation in Bacillus subtilis". PLOS Biology. 2 (10): e328. doi:10.1371/journal.pbio.0020328...

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Lysogenic cycle

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stresses. In addition to biofilm formation in B. anthracis, lysogenic conversion of Bacillus subtilis, Bacillus thuringiensis, and Bacillus cereus has...

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Genetic transformation

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Gram-positive soil bacterium Bacillus subtilis. It has also been reported in at least 30 species of Pseudomonadota distributed in several different classes...

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Palmaris longus muscle

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of Mutation Accumulation and Selection in Loss of Sporulation in Experimental Populations of Bacillus subtilis Genetics. 2007 Oct;177(2):937-48 PTCentral...

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Epulonipiscium

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spore formation. Although sporulation is widespread among other bacteria (such as Bacillus subtilis and Clostridium species) in the phylum Bacillota, spore...

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Heyndrickxia

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taxonomy of genus, resulting in the restriction of Bacillus to only include species closely related to Bacillus subtilis and Bacillus cereus, and the establishment...

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Competence factor

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observed in Sulfolobus spp. Solomon, J M (1995). "Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis"....

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Ernst Freese

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metabolic control of sporulation and germination of Bacillus subtilis bacteria. He identified the key metabolite for ignition of sporulation: a decrease of...

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Natural competence

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then, natural competence has been studied in a number of different bacteria, particularly Bacillus subtilis, Streptococcus pneumoniae (Griffith's "pneumococcus")...

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Saccharomyces boulardii

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by Bacillus subtilis or B. licheniformis contamination. S. boulardii has been shown to reduce body weight in an animal model of type 2 diabetes. In immunocompromised...

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Histidine phosphotransfer domain

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response regulator, such as the sporulation pathway in Bacillus subtilis, which can give rise to complex temporal variations. In some known cases, there is...

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