The slice preparation or brain slice is a laboratory technique in electrophysiology that allows the study of neurons from various brain regions in isolation from the rest of the brain, in an ex-vivo condition. Brain tissue is initially sliced via a tissue slicer then immersed in artificial cerebrospinal fluid (aCSF) for stimulation and/or recording.[1] The technique allows for greater experimental control, through elimination of the effects of the rest of the brain on the circuit of interest, careful control of the physiological conditions through perfusion of substrates through the incubation fluid, to precise manipulation of neurotransmitter activity through perfusion of agonists and antagonists. However, the increase in control comes with a decrease in the ease with which the results can be applied to the whole neural system.[2]
Mouse brain slices, schematically
^Schwartzkroin, Philip A. (1975). "Characteristics of CA1 neurons recorded intracellularly in the hippocampalin vitro slice preparation". Brain Research. 85 (3): 423–436. doi:10.1016/0006-8993(75)90817-3. PMID 1111846. S2CID 30478336.
^Edwards, F. A.; Konnerth, A.; Sakmann, B.; Takahashi, T. (1989). "A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system". Pflügers Archiv European Journal of Physiology. 414 (5): 600–612. doi:10.1007/BF00580998. hdl:11858/00-001M-0000-002C-2F28-1. PMID 2780225. S2CID 2616816.
The slicepreparation or brain slice is a laboratory technique in electrophysiology that allows the study of neurons from various brain regions in isolation...
method Projection-slice theorem, a theorem about Fourier transforms Bers slice in theory of Kleinian groups Slicepreparation or brain slice, an in vitro technique...
An egg slicer is a food preparation utensil used to slice peeled, hard-boiled eggs quickly and evenly. An egg slicer consists of a slotted dish for holding...
model file, in preparation for 3D printing. Slice (disambiguation) This disambiguation page lists articles associated with the title Slicing. If an internal...
A meat slicer, also called a slicing machine, deli slicer or simply a slicer, is a tool used in butcher shops and delicatessens to slice meats, sausages...
Food preparation utensils are a specific type of kitchen utensil, designed for use in the preparation of food. Some utensils are both food preparation utensils...
than from fixed tissue samples. National Society for Histotechnology Slicepreparation The word histology (/hɪstˈɒlədʒi/) is Neo-Latin using the combining...
diamond knives are also used for slicing thin sections for electron microscopy. Microtomy is a method for the preparation of thin sections for materials...
developmental biology In vitro toxicology In vitro to in vivo extrapolation Slicepreparation "In vitro methods - ECHA". echa.europa.eu. Retrieved 2023-04-11. Toxicity...
aqueous sodium cyanide injection into the whole animal and in vitro slicepreparation. These methods can be used to mimic some forms of hypoxic hypoxia...
name. Usually, takuan is washed with water to remove excess brine and then sliced thinly before serving. It is eaten as a side dish during meals, and eaten...
two slices of toasted sourdough bread, mayonnaise, pastrami, lettuce, tomato slices. Preparation involves placing the pastrami on a toasted slice of sourdough...
Maintaining healthy brain slices is pivotal for successful electrophysiological recordings. The preparation of these slices is commonly achieved with...
original Milanese preparation, cotoletta alla milanese, which is similar to the Austrian Wiener schnitzel. A milanesa is a thin slice of beef, chicken...
cakes were modifications of bread, but cakes now cover a wide range of preparations that can be simple or elaborate and which share features with desserts...
subthalamic neurons were performed using sharp electrodes in a rat slicepreparation.[citation needed] In these recordings three key observations were...
butter sandwich or jam sandwich. In basic preparation methods, a layer of peanut butter is spread on one slice of bread and a layer of fruit preserves is...
Campari, generally served on the rocks, and commonly garnished with an orange slice or orange peel. It is considered an apéritif. The drink has been documented...
equation Goldman equation Microelectrode array Planar patch clamp Slicepreparation "The Nobel Prize in Physiology or Medicine 1991". nobelprize.org....
cola. After stirring gently, the drink may also be garnished with a lemon slice. Robert Butt's recipe uses sour mix instead of lemon juice and simple syrup...
because they are frequently observed in pharmacologically treated brain slices, but are hard to measure in intact animals brains. The authors used a network...
commonly sliced, particularly for use in sandwiches. For this purpose specialized egg slicers exist, to ease slicing and yield even slices. For consistent...
International Bartenders Association recipe includes a garnish of half an orange slice and a maraschino cherry. A variant of the whiskey sour is the Ward 8, which...
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