Sequencing by ligation is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence. Unlike most currently popular DNA sequencing methods, this method does not use a DNA polymerase to create a second strand. Instead, the mismatch sensitivity of a DNA ligase enzyme is used to determine the underlying sequence of the target DNA molecule.
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Sequencingbyligation is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence...
SOLiD (Sequencingby Oligonucleotide Ligation and Detection) is a next-generation DNA sequencing technology developed by Life Technologies and has been...
structural variation in a human genome uncovered by short-read, massively parallel ligationsequencing using two-base encoding". Genome Research. 19 (9):...
lengths comparable to Illumina sequencing. This sequencingbyligation method has been reported to have some issue sequencing palindromic sequences. Ion Torrent...
created by DNA ligation. Another way, barcode-targeted sequencing, DNA circularization of a padlock probe with a barcode sequence is conducted byligation only...
polymerase-based sequence-by-synthesis on the Illumina (company) platform, or sequencingbyligation on the ABI Solid Sequencing platform. Central to miRNA-seq...
of probes that call-out a single base with each hybridization) Sequencingbyligation Drmanac, Radoje; Drmanac, Snezana; Chui, Gloria; Diaz, Robert; Hou...
released early 2018. SOLiD systems was acquired by Applied Biosystems in 2006. SOLiD applies sequencingbyligation and dual base encoding. The first SOLiD system...
Lagging strand DNA replication Okazaki fragment DNA polymerase Sequencingbyligation Pascal JM, O'Brien PJ, Tomkinson AE, Ellenberger T (November 2004)...
also called SOLiD (sequencingby oligonucleotide ligation and detection), is a next-generation sequencing technology developed by Applied Biosystems and...
By 2005, these early attempts had been overhauled to develop the existing polony sequencing technology. The highly parallel sequencing-by-ligation concepts...
genome bisulfite sequencing is a next-generation sequencing technology used to determine the DNA methylation status of single cytosines by treating the DNA...
generation HT-NGS sequencing. Three major second generation platforms include Roche/454 Pyro-sequencing, ABI/SOLiD sequencingbyligation, and Illumina’s...
Deterministic Barcoding in Tissue for Spatial Omics Sequencing (DBiT-seq) was developed at Yale University by Rong Fan and colleagues in 2020 to create a multi-omics...
in determining the success of ligation. In the last step of duplex sequencing library preparation, Illumina sequencing adapters are added to the tagged...
In ClickSeq, there are no RNA or DNA ligation steps, as are commonly required in most next generation sequencing library synthesis strategies Reduction...
at a long distance from the ligation point results in improved ligation. The main disadvantage of DNA nanoball sequencing is the short read length of...
Mindrinos, M.; Davis, R. W. (2007). "Multiplexed protein detection by proximity ligation for cancer biomarker validation". Nature Methods. 4 (4): 327–329...
Transmission electron microscopy DNA sequencing is a single-molecule sequencing technology that uses transmission electron microscopy techniques. The method...
of insert genes to destination vectors is 3:1; by increasing the insert concentration, self-ligation is further decreased. After letting the reaction...