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Sequencing by ligation information


Sequencing by ligation is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence. Unlike most currently popular DNA sequencing methods, this method does not use a DNA polymerase to create a second strand. Instead, the mismatch sensitivity of a DNA ligase enzyme is used to determine the underlying sequence of the target DNA molecule.

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Sequencing by ligation

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Sequencing by ligation is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given position in a DNA sequence...

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ABI Solid Sequencing

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SOLiD (Sequencing by Oligonucleotide Ligation and Detection) is a next-generation DNA sequencing technology developed by Life Technologies and has been...

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Massive parallel sequencing

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structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding". Genome Research. 19 (9):...

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DNA sequencing

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lengths comparable to Illumina sequencing. This sequencing by ligation method has been reported to have some issue sequencing palindromic sequences. Ion Torrent...

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Spatial transcriptomics

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created by DNA ligation. Another way, barcode-targeted sequencing, DNA circularization of a padlock probe with a barcode sequence is conducted by ligation only...

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MicroRNA sequencing

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polymerase-based sequence-by-synthesis on the Illumina (company) platform, or sequencing by ligation on the ABI Solid Sequencing platform. Central to miRNA-seq...

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Sequencing by hybridization

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of probes that call-out a single base with each hybridization) Sequencing by ligation Drmanac, Radoje; Drmanac, Snezana; Chui, Gloria; Diaz, Robert; Hou...

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DNA sequencer

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released early 2018. SOLiD systems was acquired by Applied Biosystems in 2006. SOLiD applies sequencing by ligation and dual base encoding. The first SOLiD system...

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DNA ligase

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Lagging strand DNA replication Okazaki fragment DNA polymerase Sequencing by ligation Pascal JM, O'Brien PJ, Tomkinson AE, Ellenberger T (November 2004)...

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2 base encoding

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also called SOLiD (sequencing by oligonucleotide ligation and detection), is a next-generation sequencing technology developed by Applied Biosystems and...

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Polony sequencing

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By 2005, these early attempts had been overhauled to develop the existing polony sequencing technology. The highly parallel sequencing-by-ligation concepts...

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Whole genome bisulfite sequencing

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genome bisulfite sequencing is a next-generation sequencing technology used to determine the DNA methylation status of single cytosines by treating the DNA...

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Cancer genome sequencing

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generation HT-NGS sequencing. Three major second generation platforms include Roche/454 Pyro-sequencing, ABI/SOLiD sequencing by ligation, and Illumina’s...

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Deterministic Barcoding in Tissue for Spatial Omics Sequencing

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Deterministic Barcoding in Tissue for Spatial Omics Sequencing (DBiT-seq) was developed at Yale University by Rong Fan and colleagues in 2020 to create a multi-omics...

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Positional sequencing

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and chromosomal aneuploidies. Sequencing by ligation Heger, Monica (2012-01-17). "Nabsys Develops 'Positional Sequencing' Tech for Assembly, Whole-Genome...

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Duplex sequencing

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in determining the success of ligation. In the last step of duplex sequencing library preparation, Illumina sequencing adapters are added to the tagged...

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Transposon sequencing

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Transposon insertion sequencing (Tn-seq) combines transposon insertional mutagenesis with massively parallel sequencing (MPS) of the transposon insertion...

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ClickSeq

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In ClickSeq, there are no RNA or DNA ligation steps, as are commonly required in most next generation sequencing library synthesis strategies Reduction...

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DNA nanoball sequencing

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at a long distance from the ligation point results in improved ligation. The main disadvantage of DNA nanoball sequencing is the short read length of...

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Proximity extension assay

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Mindrinos, M.; Davis, R. W. (2007). "Multiplexed protein detection by proximity ligation for cancer biomarker validation". Nature Methods. 4 (4): 327–329...

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Transmission electron microscopy DNA sequencing

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Transmission electron microscopy DNA sequencing is a single-molecule sequencing technology that uses transmission electron microscopy techniques. The method...

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Subcloning

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of insert genes to destination vectors is 3:1; by increasing the insert concentration, self-ligation is further decreased. After letting the reaction...

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