Deterministic Barcoding in Tissue for Spatial Omics Sequencing information

Deterministic Barcoding in Tissue for Spatial Omics Sequencing (DBiT-seq) was developed at Yale University by Rong Fan and colleagues in 2020 to create a multi-omics approach for studying spatial gene expression heterogenicity within a tissue sample.^[1] This method can be used for the co-mapping mRNA and protein levels at a near single-cell resolution in fresh or frozen formaldehyde-fixed tissue samples. DBiT-seq utilizes next generation sequencing (NGS) and microfluidics. This method allows for simultaneous spatial transcriptomic and proteomic analysis of a tissue sample. DBiT-seq improves upon previous spatial transcriptomics applications such as High-Definition Spatial Transcriptomics (HDST) and Slide-seq by increasing the number of detectable genes per pixel, increased cellular resolution, and ease of implementation.^[1]^[2]^[3]

^ ^a ^b Liu, Yang; Yang, Mingyu; Deng, Yanxiang; Su, Graham; Enninful, Archibald; Guo, Cindy C.; Tebaldi, Toma; Zhang, Di; Kim, Dongjoo; Bai, Zhiliang; Norris, Eileen (2020-12-10). "High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue". Cell. 183 (6): 1665–1681.e18. doi:10.1016/j.cell.2020.10.026. ISSN 0092-8674. PMC 7736559. PMID 33188776.
^ Rodriques, Samuel G.; Stickels, Robert R.; Goeva, Aleksandrina; Martin, Carly A.; Murray, Evan; Vanderburg, Charles R.; Welch, Joshua; Chen, Linlin M.; Chen, Fei; Macosko, Evan Z. (2019-03-29). "Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution". Science. 363 (6434): 1463–1467. Bibcode:2019Sci...363.1463R. doi:10.1126/science.aaw1219. ISSN 1095-9203. PMC 6927209. PMID 30923225.
^ Vickovic, Sanja; Eraslan, Gökcen; Salmén, Fredrik; Klughammer, Johanna; Stenbeck, Linnea; Schapiro, Denis; Äijö, Tarmo; Bonneau, Richard; Bergenstråhle, Ludvig; Navarro, José Fernandéz; Gould, Joshua (October 2019). "High-definition spatial transcriptomics for in situ tissue profiling". Nature Methods. 16 (10): 987–990. doi:10.1038/s41592-019-0548-y. ISSN 1548-7105. PMC 6765407. PMID 31501547.

[:0-1] Liu, Yang; Yang, Mingyu; Deng, Yanxiang; Su, Graham; Enninful, Archibald; Guo, Cindy C.; Tebaldi, Toma; Zhang, Di; Kim, Dongjoo; Bai, Zhiliang; Norris, Eileen (2020-12-10). "High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue". Cell. 183 (6): 1665–1681.e18. doi:10.1016/j.cell.2020.10.026. ISSN 0092-8674. PMC 7736559. PMID 33188776.

[:1-2] Rodriques, Samuel G.; Stickels, Robert R.; Goeva, Aleksandrina; Martin, Carly A.; Murray, Evan; Vanderburg, Charles R.; Welch, Joshua; Chen, Linlin M.; Chen, Fei; Macosko, Evan Z. (2019-03-29). "Slide-seq: A scalable technology for measuring genome-wide expression at high spatial resolution". Science. 363 (6434): 1463–1467. Bibcode:2019Sci...363.1463R. doi:10.1126/science.aaw1219. ISSN 1095-9203. PMC 6927209. PMID 30923225.

[3] Vickovic, Sanja; Eraslan, Gökcen; Salmén, Fredrik; Klughammer, Johanna; Stenbeck, Linnea; Schapiro, Denis; Äijö, Tarmo; Bonneau, Richard; Bergenstråhle, Ludvig; Navarro, José Fernandéz; Gould, Joshua (October 2019). "High-definition spatial transcriptomics for in situ tissue profiling". Nature Methods. 16 (10): 987–990. doi:10.1038/s41592-019-0548-y. ISSN 1548-7105. PMC 6765407. PMID 31501547.