molecular biology, subcloning is a technique used to move a particular DNA sequence from a parent vector to a destination vector. Subcloning is not to be confused...
strand. The concept is used in molecular biology, in cloning, or when subcloning insert DNA into vector DNA. Such ends may be generated by restriction...
T cloning) is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than traditional subcloning. The technique relies...
genomes. Cancers usually show tumour heterogeneity, containing multiple subclones. They also frequently have reduced expression of DNA repair enzymes due...
electrophoresis, and chromatography. It is used in genetic fingerprinting, plasmid subcloning, and RFLP analysis. A given restriction enzyme cuts DNA segments within...
ob/ob mice FBS+ I+ T3 TA1 Subclone of C3H10T1/2 FBS + D + I 30A5 Subclone of C3H10T1/2 FBS + D + M + I 1246 Adipogenic Subclone of CH3 mouse teratocarcinoma...
may give rise to subclones that possess an evolutionary advantage over the others within the tumour environment, and these subclones may become dominant...
the intensity, and high heterogeneity - variability - of tumor cell subclones. Phylogenetics can also aid in drug design and discovery. Phylogenetics...
were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation...
experimental observations. That particular strand-biased divergent domain was subcloned and its altered helical structure was studied in greater detail. A fifth...
DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use. There are many types...
experiments usually begin with cloning into a bacterial plasmid, followed by subcloning into a specialized vector. Whatever combination of host and vector are...
1962 cell line 3T3-J2, a subclone of 3T3-Swiss albino, commonly used as feeders for keratinocyte cultures 3T3-L1, a subclone of 3T3-Swiss albino, used...
yielding transformation efficiencies that are adequate for cloning and subcloning applications, approximately 106 CFU/µg. One of the early methods used...
is an immortalized mouse myoblast cell line. The C2C12 cell line is a subclone of myoblasts that were originally obtained by Yaffe and Saxel at the Weizmann...
fragment containing all assembled genes to be excised from the vector and subcloned in a next level of cloning (Level P). Level P vectors are similar to level...
10 or Silver Standard) within existing plasmids (i.e. without PCR or subcloning). The plasmids are reacted in vivo with sequence-specific DNA methyltransferases...
DNA fragmentation is often necessary prior to library construction or subcloning for DNA sequences. A variety of methods involving the mechanical breakage...
receptor or in its enhancer, mostly, suggesting the presence of different subclones with different genotypes of these receptors. Androgen receptor has been...
fragment during southern blot. The name "p13E-11" reflects that it is a subclone of a DNA sequence designated as cosmid 13E during the human genome project...
example, a DNA sequence for a protein of interest could be cloned or subcloned into a high copy-number plasmid containing the lac (often LacUV5) promoter...
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