Secretion assay is a process used in cell biology to identify cells that are secreting a particular protein (usually a cytokine). It was first developed by Manz et al. in 1995.
Usually, a cell that is secreting the protein of interest is isolated using an antibody-antibody complex that coats the cell and is able to "catch" the secreted molecules. The cell is then detected by another fluorochrome-labelled antibody, and is subsequently extracted using a process called fluorescent-activated cell sorting (FACS). The FACS method is broadly similar to the enzyme-linked immunosorbent assay (ELISA) antibody format, except that the encapsulated cells remain intact. This is advantageous as the cells are still living after the extraction has taken place.
Further advances now mean that it is possible to extract the secreting cells using a magnetic-based separation system or using a flow cytometer.
A number of commercial applications exist for secretion assay. One such example is the Gel Microdrop (GMD) technology, developed by One Cell Systems. One Cell asserts that GMD typically recovers a higher number of viable secreting cells than other methods, whilst ignoring any cells which are not secreting the desired protein.
Secretionassay is a process used in cell biology to identify cells that are secreting a particular protein (usually a cytokine). It was first developed...
The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzə/, /ˌiːˈlaɪzə/) is a commonly used analytical biochemistry assay, first described by Eva Engvall...
proteins it generates, other scientific techniques like the ELISPOT or secretionassay techniques can be used. In the circulatory system, they move from lymph...
The type III secretion system (T3SS or TTSS) is one of the bacterial secretion systems used by bacteria to secrete their effector proteins into the host's...
(ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell. The ELISpot Assay is also a form...
to decreased kidney function, to interference with the assay, or to decreased tubular secretion of creatinine. An increase in serum creatinine can be due...
decreased affinity of "Big" prolactin in a radioreceptor assay and evidence for its secretion". The Journal of Clinical Endocrinology and Metabolism. 47...
cervix. The rest is made up of transudate from the vaginal walls and secretions from glands (Skene's and Bartholin's). The solid components or elements...
that has effects like gastrin when given parenterally. It stimulates the secretion of gastric acid, pepsin, and intrinsic factor, and has been used as a...
influence results. The assay is also referred to as ejaculate analysis, human sperm assay (HSA), sperm function test, and sperm assay. Semen analysis is a...
lymphocyte secretions (ALS) assay is an immunological assay to detect active diseases like tuberculosis, cholera, typhoid etc. Recently, ALS assay nods the...
effects of diuretic factors are tested in fluid secretion experiments, usually conducted using the Ramsay assay, in which isolated Malpighian tubules are placed...
their environment, and these organisms can be used to conduct laboratory assays to identify the presence and function of such exoenzymes. Some pathogenic...
derivate radio-enzymatic assays (REA) transformed the analysis down to a sensitivity of 1 pg for adrenaline. Early REA plasma assays indicated that adrenaline...
A tetramer assay (also known as a tetramer stain) is a procedure that uses tetrameric proteins to detect and quantify T cells that are specific for a...
porous surfaces such as paper. The amino acids present in the minute sweat secretions that gather on the finger's unique ridges transfer to surfaces that are...
synthesis and secretion of catecholamines from the adrenal chromaffin cells. This chronic increase of epinephrine and norepinephrine secretion causes desensitization...
High-potassium media (which stimulates aldosterone secretion in vitro) also stimulate cortisol secretion from the fasciculata zone of canine adrenals — unlike...
increase their rate of nutrient transfer, and increase their protease secretion. Transformation can also affect the cytoskeleton and change in the quantity...
Springer, 1, 282. ISBN 0-306-46114-5. Svendsen, G.E., Huntsman, W.D, "A field Assay of Beaver Castoreum and Some of its Components". American Midland Naturalist...
Plasma renin activity (PRA), also known as the renin (active) assay or random plasma renin, is a measure of the activity of the plasma enzyme renin, which...
production). The Insulin tolerance test is recognized as the gold standard assay of adrenal insufficiency, but due to the cumbersome requirement for a two-hour...
from relatively simple membrane assays. like vesicular transport assay, ATPase assay to more complex cell based assays up to intricate in vivoJeffrey P...
the main immunoglobulin found in mucous secretions, including tears, saliva, sweat, colostrum and secretions from the genitourinary tract, gastrointestinal...
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