Recombinant AAV mediated genome engineering information
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Recombinant adeno-associated virus (rAAV) based genome engineering is a genome editing platform centered on the use of recombinant AAV vectors that enables insertion, deletion or substitution of DNA sequences into the genomes of live mammalian cells. The technique builds on Mario Capecchi and Oliver Smithies' Nobel Prize–winning discovery that homologous recombination (HR), a natural hi-fidelity DNA repair mechanism, can be harnessed to perform precise genome alterations in mice. rAAV mediated genome-editing improves the efficiency of this technique to permit genome engineering in any pre-established and differentiated human cell line, which, in contrast to mouse ES cells, have low rates of HR.
The technique has been widely adopted for use in engineering human cell lines to generate isogenic human disease models. It has also been used to optimize bioproducer cell lines for the biomanufacturing of protein vaccines and therapeutics. In addition, due to the non-pathogenic nature of rAAV, it has emerged as a desirable vector for performing gene therapy in live patients.
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Recombinant adeno-associated virus (rAAV) based genomeengineering is a genome editing platform centered on the use of recombinantAAV vectors that enables...
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: 4 Genetic material from AAV vectors is integrated into the host cell's nuclear genome at a low frequency and likely mediated by the DNA-modifying enzymes...
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adeno-associated viruses (AAVs), adenoviruses, and lentiviruses. With adeno-associated viruses and adenoviruses, the genomes remain episomal. This is advantageous...
integration into the genome with recombinant vectors, (5) risk of insertional genotoxicity, and (6) limited packaging capacity (Adeno and AAV typically restricted...
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reasons and until a real mechanism can be found. Oncolytic virus Oncolytic AAV Oncolytic herpes virus Virotherapy Pandha K. J. Harrington; edited by Richard...
for the Production of Recombinant Proteins". Advances in Animal and Veterinary Sciences. 4 (7): 346–356. doi:10.14737/journal.aavs/2016/4.7.346.356. ISSN 2307-8316...
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