This article is about direct or indirect binding. For grouping according to time of debut, see Regular and irregular antibodies.
Primary and secondary antibodies are two groups of antibodies that are classified based on whether they bind to antigens or proteins directly or target another (primary) antibody that, in turn, is bound to an antigen or protein.
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Primaryandsecondaryantibodies are two groups of antibodies that are classified based on whether they bind to antigens or proteins directly or target...
unconjugated (unlabeled). Secondaryantibodies are raised against immunoglobulins of the primaryantibody species. The secondaryantibody is usually conjugated...
the antigen. Enzyme-linked secondaryantibodies are applied as detection antibodies, which bind specifically to the antibody's Fc region (nonspecific)....
anti-histone antibodies, antibodies to nuclear pore complexes, anti-centromere antibodiesand anti-sp100 antibodies. Each of these antibody subtypes binds to...
using a general secondaryantibody that recognises all antibodies originating from same animal species as the primary. The secondaryantibody is tagged with...
specificity of antibodiesand antigens. The specific region an antibody recognizes on an antigen is called an epitope. Several antibodies can recognize...
Detection of antibodies (cold or warm) and /or complement system on RBC from the patient is a direct Coombs antiglobulin test. Detection of antibodies in serum...
secondary antibody mixtures, where secondary fragment antigen-binding antibodies are frequently used. Secondly, haptenylated primaryantibodies can be used...
gold particles are most often attached to secondaryantibodies which are in turn attached to primaryantibodies designed to bind a specific antigen or other...
targeted neurotransmitter could be specifically tagged by primaryandsecondaryantibodies with radioactive labeling in order to identify the neurotransmitter...
using either a primary or a secondary labeled antibody by chemiluminescent, fluorescent or colorimetric assays. The array is then imaged and the obtained...
Polyclonal antibodies (pAbs) are antibodies that are secreted by different B cell lineages within the body (whereas monoclonal antibodies come from a single...
antiphospholipid antibodies that are thought to clinically cause disease. These antibodies lead to blood clots and vascular disease in the presence (secondary APS)...
signs and symptoms of syphilis vary depending in which of the four stages it presents (primary, secondary, latent, and tertiary). The primary stage classically...
(IgG) is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation...
matter and aged blood cells, mainly red blood cells to produce blood cells during fetal life. The spleen synthesizes antibodies in its white pulp and removes...
is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This process starts by injecting a mouse (or other...
produce particular pathophysiologic signs and symptoms, are more prevalent. Primary systemic, secondary, and single-organ vasculitis are distinguished...
emotional stress. Primary Raynaud's is idiopathic (spontaneous and of unknown cause) and not correlated with another disease. Secondary Raynaud's occurs...
produce antibodies of successively greater affinities. A secondary response can elicit antibodies with several fold greater affinity than in a primary response...
are two types of active transport: primary active transport that uses adenosine triphosphate (ATP), andsecondary active transport that uses an electrochemical...
target antigen, with a secondary antibody directed against the primary, and a tag attached to the secondaryantibody. Commercial DFA testing kits are...