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Primary and secondary antibodies information


Primary and secondary antibodies are two groups of antibodies that are classified based on whether they bind to antigens or proteins directly or target another (primary) antibody that, in turn, is bound to an antigen or protein.

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Primary and secondary antibodies

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Primary and secondary antibodies are two groups of antibodies that are classified based on whether they bind to antigens or proteins directly or target...

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Western blot

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and marking target protein using a primary and secondary antibody to visualize. A synthetic or animal-derived antibody (known as the primary antibody)...

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Immunohistochemistry

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unconjugated (unlabeled). Secondary antibodies are raised against immunoglobulins of the primary antibody species. The secondary antibody is usually conjugated...

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ELISA

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the antigen. Enzyme-linked secondary antibodies are applied as detection antibodies, which bind specifically to the antibody's Fc region (nonspecific)....

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Antinuclear antibody

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anti-histone antibodies, antibodies to nuclear pore complexes, anti-centromere antibodies and anti-sp100 antibodies. Each of these antibody subtypes binds to...

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Immunoperoxidase

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using a general secondary antibody that recognises all antibodies originating from same animal species as the primary. The secondary antibody is tagged with...

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Immunofluorescence

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specificity of antibodies and antigens. The specific region an antibody recognizes on an antigen is called an epitope. Several antibodies can recognize...

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Cold agglutinin disease

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Detection of antibodies (cold or warm) and /or complement system on RBC from the patient is a direct Coombs antiglobulin test. Detection of antibodies in serum...

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Immunolabeling

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secondary antibody mixtures, where secondary fragment antigen-binding antibodies are frequently used. Secondly, haptenylated primary antibodies can be used...

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Immunogold labelling

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gold particles are most often attached to secondary antibodies which are in turn attached to primary antibodies designed to bind a specific antigen or other...

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Molecular neuroscience

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targeted neurotransmitter could be specifically tagged by primary and secondary antibodies with radioactive labeling in order to identify the neurotransmitter...

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Reverse phase protein lysate microarray

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using either a primary or a secondary labeled antibody by chemiluminescent, fluorescent or colorimetric assays. The array is then imaged and the obtained...

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Polyclonal antibodies

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Polyclonal antibodies (pAbs) are antibodies that are secreted by different B cell lineages within the body (whereas monoclonal antibodies come from a single...

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Antiphospholipid syndrome

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antiphospholipid antibodies that are thought to clinically cause disease. These antibodies lead to blood clots and vascular disease in the presence (secondary APS)...

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Invitrogen

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CellzDirect (cell products and services for research) and Zymed and Caltag Laboratories (primary and secondary antibodies) have been brought under the...

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Syphilis

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signs and symptoms of syphilis vary depending in which of the four stages it presents (primary, secondary, latent, and tertiary). The primary stage classically...

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Index of immunology articles

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Polyclonal antibodies Polyclonal B cell response Polymersome Pox party Precipitin Premunition Premunity Primary and secondary antibodies Priming (immunology)...

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Immunoglobulin G

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(IgG) is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation...

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Lymphatic system

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matter and aged blood cells, mainly red blood cells to produce blood cells during fetal life. The spleen synthesizes antibodies in its white pulp and removes...

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Hybridoma technology

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is a method for producing large numbers of identical antibodies (also called monoclonal antibodies). This process starts by injecting a mouse (or other...

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Vasculitis

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produce particular pathophysiologic signs and symptoms, are more prevalent. Primary systemic, secondary, and single-organ vasculitis are distinguished...

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Raynaud syndrome

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emotional stress. Primary Raynaud's is idiopathic (spontaneous and of unknown cause) and not correlated with another disease. Secondary Raynaud's occurs...

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Affinity maturation

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produce antibodies of successively greater affinities. A secondary response can elicit antibodies with several fold greater affinity than in a primary response...

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Active transport

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are two types of active transport: primary active transport that uses adenosine triphosphate (ATP), and secondary active transport that uses an electrochemical...

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Direct fluorescent antibody

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target antigen, with a secondary antibody directed against the primary, and a tag attached to the secondary antibody. Commercial DFA testing kits are...

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