DNA sequence required in bacterial promoters for transcription
The Pribnow box (also known as the Pribnow-Schaller box) is a sequence of TATAAT of six nucleotides (thymine, adenine, thymine, etc.) that is an essential part of a promoter site on DNA for transcription to occur in bacteria.[1][2] It is an idealized or consensus sequence—that is, it shows the most frequently occurring base at each position in many promoters analyzed; individual promoters often vary from the consensus at one or more positions. It is also commonly called the -10 sequence or element, because it is centered roughly ten base pairs upstream from the site of initiation of transcription.
The Pribnow box has a function similar to the TATA box that occurs in promoters in eukaryotes and archaea: it is recognized and bound by a subunit of RNA polymerase during initiation of transcription.[3] This region of the DNA is also the first place where base pairs separate during prokaryotic transcription to allow access to the template strand. The AT-richness is important to allow this separation, since adenine and thymine are easier to break apart (not only due to fewer hydrogen bonds, but also due to weaker base stacking effects).[4]
It is named after David Pribnow and Heinz Schaller.[citation needed][1][2]
^ ab
Pribnow, David (March 1975). "Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter". PNAS. 72 (3): 784–788. Bibcode:1975PNAS...72..784P. doi:10.1073/pnas.72.3.784. ISSN 1091-6490. PMC 432404. PMID 1093168.
^ ab
Schaller, Heinz; Gray, Christopher; Herrman, Karin (February 1975). "Nucleotide sequence of an RNA polymerase binding site from the DNA of Bacteriophage fd". PNAS. 72 (2): 737–741. Bibcode:1975PNAS...72..737S. doi:10.1073/pnas.72.2.737. ISSN 1091-6490. PMC 432391. PMID 1054851.
^Feklistov, Andrey; Darst, Seth (2011). "Structural Basis for Promoter −10 Element Recognition by the Bacterial RNA Polymerase σ Subunit". Cell. 147 (6): 1257–69. doi:10.1016/j.cell.2011.10.041. PMC 3245737. PMID 22136875.
^
Yakovchuk, Peter; Protozanova, Ekaterina; Frank-Kamenetskii, Maxim D. (January 2006). "Base-stacking and base-pairing contributions into thermal stability of the DNA double helix". Nucleic Acids Research. 34 (2): 564–574. doi:10.1093/nar/gkj454. ISSN 0305-1048. PMC 1360284. PMID 16449200.
The Pribnowbox (also known as the Pribnow-Schaller box) is a sequence of TATAAT of six nucleotides (thymine, adenine, thymine, etc.) that is an essential...
The bacterial homolog of the TATA box is called the Pribnowbox which has a shorter consensus sequence. The TATA box is considered a non-coding DNA sequence...
references to biology, particularly genetics, including polysomes, the Pribnowbox, the central dogma of molecular biology and the sigma factor. Its title...
Region 2.4 recognizes and binds to the promoter −10 element (called the "Pribnowbox"). Region 4.2 recognizes and binds to the promoter −35 element. Not every...
is a core promoter that is similar in function to the Pribnowbox (in prokaryotes) or the TATA box (in eukaryotes). The Inr is the simplest functional promoter...
the RNA polymerase holoenzyme. Part of the Qut site resembles the -10 Pribnowbox, causing the holoenzyme to pause. Q protein then binds and displaces...
the DNA double helix that need to separate easily, such as the TATAAT Pribnowbox in some promoters, tend to have a high AT content, making the strands...
relatively well-understood promoter sequences (signals), such as the Pribnowbox and transcription factor binding sites, which are easy to systematically...
metabolic pathogenic genes, as a result of mutations introducing new TANNNT Pribnowboxes and mutations that impair the function of transcriptional repressors...
type of RNA polymerase, which needs to bind a DNA sequence called a Pribnowbox with the help of the sigma factor protein (σ factor) to start transcription...
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