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Polygalacturonase information


Endo-polygalacturonase
Computer generated image of Polygalacturonase as found in Aspergillus aculeatus (1IA5) at pH 8.5[1]
Identifiers
EC no.3.2.1.15
CAS no.9032-75-1
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MetaCycmetabolic pathway
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Endo-polygalacturonase (EC 3.2.1.15, pectin depolymerase, pectolase, pectin hydrolase, and poly-α-1,4-galacturonide glycanohydrolase; systematic name (1→4)-α-D-galacturonan glycanohydrolase (endo-cleaving)) is an enzyme that hydrolyzes the α-1,4 glycosidic bonds between galacturonic acid residues:

(1,4-α-D-galacturonosyl)n+m + H2O = (1,4-α-D-galacturonosyl)n + (1,4-α-D-galacturonosyl)m

Polygalacturonan, whose major component is galacturonic acid,[2] is a significant carbohydrate component of the pectin network that comprises plant cell walls.[3] Therefore, the activity of the endogenous plant PGs works to soften and sweeten fruit during the ripening process. Similarly, phytopathogens use PGs as a means to weaken the pectin network, so that digestive enzymes can be excreted into the plant host to acquire nutrients.

  1. ^ Cho SW, Lee S, Shin W (August 2001). "The X-ray structure of Aspergillus aculeatus polygalacturonase and a modeled structure of the polygalacturonase-octagalacturonate complex". Journal of Molecular Biology. 311 (4): 863–78. doi:10.1006/jmbi.2001.4919. PMID 11518536.
  2. ^ "Medical Definition of Polygalacturonan". Lexic.us.
  3. ^ Jones, T. M., Anderson, A. J., and Albersheim, P. (1972) Host–pathogen interactions IV, Studies on the polysaccharide-degrading enzymes secreted by Fusarium oxysporum f. sp. lycopersici, Physiol. Plant Pathol. 2, 153-166.

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Polygalacturonase

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Endo-polygalacturonase (EC 3.2.1.15, pectin depolymerase, pectolase, pectin hydrolase, and poly-α-1,4-galacturonide glycanohydrolase; systematic name...

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Pectinase

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referred to as pectic enzymes, they include pectolyase, pectozyme, and polygalacturonase, one of the most studied and widely used[citation needed] commercial...

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Polygalacturonase inhibitor

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Polygalacturonase inhibitor proteins (PGIPs), also known as polygalacturonase-inhibiting proteins, are plant proteins capable of inhibiting the action...

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Genetically modified tomato

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copy of the tomato gene polygalacturonase was inserted into the tomato genome in the antisense direction. The polygalacturonase enzyme degrades pectin...

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Flavr Savr

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Flavr Savr contains two genes added by Calgene; a reversed antisense polygalacturonase gene which inhibits the production of a rotting enzyme and a gene...

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Calcium propanoate

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El-Neshawy, S.; Nickerson, R. (1997). "Effects of Calcium Salts on Growth, Polygalacturonase Activity, and Infection of Peach Fruit by Monilinia fructicola". Plant...

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Genetic engineering

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(1 January 1994). "Commercialization of a tomato with an antisense polygalacturonase gene: The FLAVR SAVR™ tomato story". Euphytica. 79 (3): 293–97. doi:10...

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Glycoside hydrolase family 28

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with several known activities; polygalacturonase (EC 3.2.1.15); exo-polygalacturonase (EC 3.2.1.67); exo-polygalacturonase (EC 3.2.1.82); rhamnogalacturonase...

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PG

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Columbia, Canada Prince George's County, Maryland, or PG County, U.S. Polygalacturonase, an enzyme Propylene glycol, an organic compound Prostaglandin, physiologically...

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Penicillium olsonii

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filamentous species in the genus Penicillium which produces several polygalacturonases, xanthoepocin, asperphenamate, verrucolone, phthalate and olnacin...

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Ripening

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a soluble one by certain degrading enzymes. These enzymes include polygalacturonase. This means that the fruit will become less firm as the structure...

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Abscission

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gene expression of cell wall-degrading enzymes such as cellulase and polygalacturonase are activated. However, this is not to say that ethylene directly...

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Hemiptera

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into the body). These enzymes include amylase to hydrolyse starch, polygalacturonase to weaken the tough cell walls of plants, and proteinases to break...

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Cider

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they may contain enzymes, such as β-glucosidase, β-xylosidase, or polygalacturonase, which Saccharomyces yeast may not produce. Not all yeast associated...

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Klebsiella michiganensis

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is negative in the pectin degradation test and negative by PCR for polygalacturonase gene pehX. The name K. michiganensis comes from the United States'...

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Pectolase

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Pectolase may refer to one of two enzymes: Pectin lyase Polygalacturonase This set index page lists enzyme articles associated with the same name. If...

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Pasteurization

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treatments are designed to inactivate enzymes (pectin methylesterase and polygalacturonase in fruit juices) and destroy spoilage microbes (yeast and lactobacillus)...

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Janus kinase inhibitor

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(EC 3) 3.1 Phosphodiesterase Acetylcholinesterase Ribonuclease 3.2 Polygalacturonase Neuraminidase Alpha-glucosidase 3.4 Protease: Exopeptidase DPP-4 ACE...

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Turnera subulata

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far, it has been established that short styles contain proteins, polygalacturonases, that are absent in long styles. This plant has uses in traditional...

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Rhizopus oryzae

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enzymes such as protease, urease, ribonuclease, pectate lyase, and polygalacturonase are found in cultural media of R. oryzae. Besides producing a number...

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Mrakia aquatica

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produce pecticase. It produces polycalacturonase, but not isoenzymes of polygalacturonase, and it has increased activity in the presence of glucose. The species...

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Tarnished plant bug

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into the host plant. The saliva of the TPB contains an enzyme called polygalacturonase which degrades plant tissue and pectin in the plant cell wall allowing...

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Food irradiation

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result of temporary inactivation of self-degradation enzymes like polygalacturonase and increased expression of enzymes associated with cell wall repair...

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Coconut oil

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extraction of coconut oil involves the enzymatic action of alpha-amylase, polygalacturonases, and proteases on diluted coconut paste. Unlike virgin coconut oil...

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Penicillium solitum

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genus Penicillium. It is known to produce various compounds including polygalacturonase, compactin, cyclopenin, cyclopenol, cyclopeptin, dehydrocompactin...

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Ctenolepisma longicaudatum

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is very low in the head, but the enzyme is present in the foregut. Polygalacturonase activity is not observed in C. longicaudatum. A cellulose-rich diet...

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BURP domain

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function in cortex parenchyma. Tomato PG1beta, the beta-subunit of polygalacturonase isozyme 1 (PG1), which is expressed in ripening fruits. Cereal RAFTIN...

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Burkholderia cenocepacia

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a variety of cellular processes, such as extracellular proteases, polygalacturonase, and the production of siderophores. Burkholderia cenocepacia is one...

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