Native Chemical Ligation (NCL) is an important extension of the chemical ligation concept for constructing a larger polypeptide chain by the covalent condensation of two or more unprotected peptides segments.[1] Native chemical ligation is the most effective method for synthesizing native or modified proteins of typical size (i.e., proteins< ~300 AA).[2]
^Dawson, P. E.; Muir, T. W.; Clark-Lewis, I.; Kent, S. B. (1994). "Synthesis of proteins by native chemical ligation". Science. 266 (5186): 776–778. Bibcode:1994Sci...266..776D. doi:10.1126/science.7973629. PMID 7973629.
^Agouridas V, El Mahdi O, Diemer V, Cargoët M, Monbaliu JM, Melnyk O (June 2019). "Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations". Chemical Reviews. 119 (12): 7328–7443. doi:10.1021/acs.chemrev.8b00712. PMID 31050890. S2CID 145023266.
and 27 Related for: Native chemical ligation information
Chemicalligation is the chemoselective condensation of unprotected peptide segments enabled by the formation of a non-native bond at the ligation site...
fragments made by synthesis, chemical biologists can use the process of nativechemicalligation. Nativechemicalligation involves the coupling of a C-terminal...
Protein chemical synthesis by native peptide ligation of unprotected peptide segments is an interesting complement and potential alternative to the use...
traditional resin-based solid phase techniques would be better. Nativechemicalligation (NCL) is a convergent synthetic strategy based on the linear coupling...
oxygen nucleophiles, giving amides: This reaction is exploited in nativechemicalligation, a protocol for peptide synthesis. In a related reaction, thioesters...
occurring analogy to the technique for chemically generating medium-sized proteins called nativechemicalligation. An intein is a segment of a protein...
to make. Longer lengths can be accessed by using ligation approaches such as nativechemicalligation, where two shorter fully deprotected synthetic peptides...
video game company Northern Coalfields, Indian coal company Nativechemicalligation, a chemical way to synthesize proteins Neuronal ceroid lipofuscinosis...
Amide-Forming Ligation is a chemical reaction that is used to join two unprotected fragments in peptide synthesis. It is an alternative to the NativeChemical Ligation...
the L-protein and the D-protein are prepared by total chemical synthesis. Nativechemicalligation of unprotected peptide segments is used to prepare the...
T. (2001). "Selenocysteine in NativeChemicalLigation and Expressed Protein Ligation". Journal of the American Chemical Society. 123 (21): 5140–5141....
group for subsequent ligation cycles (chained ligation) or by removing and hybridizing a new primer to the template (unchained ligation). Pacific Biosciences...
variety of proteins have been obtained by chemical synthesis. A successful approach relies on nativechemicalligation, which achieves the synthesis of proteins...
real time in living systems without cellular toxicity. A number of chemicalligation strategies have been developed that fulfill the requirements of bioorthogonality...
reaction in Figure 4). An example of C-termini modification is the nativechemicalligation (NCL), which is the coupling between a C-terminal thioester and...
real time in living systems without cellular toxicity. A number of chemicalligation strategies have been developed that fulfill the requirements of bioorthogonality...
synthesized chemically by a family of methods known as peptide synthesis, which rely on organic synthesis techniques such as chemicalligation to produce...
PMID 12458208. Lu M, Min T, Eliezer D, Wu H (February 2006). "Nativechemicalligation in covalent caspase inhibition by p35". Chemistry & Biology. 13...
libraries. A peptide chain of 10 residues in length is used in nativechemicalligation with a larger recombinantly expressed protein. Residue 1: alanine...
knowledge or consent, including the use of Depo Provera, as well as tubal ligations. Both forced adoption and forced contraception would fall under the provisions...
procedures by government officials. In 2004, about 1/3 of Native American women were undergoing tubal ligation, a rate that is 123% greater than for white women...
radiologist. There is no difference in outcomes between embolization and ligation as treatment options, but embolization is considerably more expensive....
virus (TRSV) satellite RNA where it catalyzes self-cleavage and joining (ligation) reactions to process the products of rolling circle virus replication...
vector avoids toxicity and counter-selection that would be introduced by ligation and propagation in E. coli.[page needed] This method introduces mutations...
birth control are sterilization by means of vasectomy in males and tubal ligation in females, intrauterine devices (IUDs), and implantable birth control...
Global Information