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Microarray analysis techniques information


Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail.

Microarray analysis techniques are used in interpreting the data generated from experiments on DNA (Gene chip analysis), RNA, and protein microarrays, which allow researchers to investigate the expression state of a large number of genes – in many cases, an organism's entire genome – in a single experiment.[1] Such experiments can generate very large amounts of data, allowing researchers to assess the overall state of a cell or organism. Data in such large quantities is difficult – if not impossible – to analyze without the help of computer programs.

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Microarray analysis techniques

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Microarray analysis techniques are used in interpreting the data generated from experiments on DNA (Gene chip analysis), RNA, and protein microarrays...

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DNA microarray

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Transcriptomics technologies Serial analysis of gene expression RNA-Seq MAGIChip Microarray analysis techniques Microarray databases Cyanine dyes, such as...

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Microarray

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carbohydrate microarrays is expanding. Types of microarrays include: DNA microarrays, such as cDNA microarrays, oligonucleotide microarrays, BAC microarrays and...

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Analytical chemistry

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sequencing and related research in genetic fingerprinting and DNA microarray; proteomics, the analysis of protein concentrations and modifications, especially in...

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Protein microarray

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A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function...

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Microarray databases

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available to other applications for analysis and interpretation (either directly, or via user downloads). Microarray databases can fall into two distinct...

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Gene expression profiling

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data to analyse. DNA microarrays measure the relative activity of previously identified target genes. Sequence based techniques, like RNA-Seq, provide...

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Tissue microarray

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of diagnostic reagents and limited patient sample size. The technique of tissue microarray was developed to address these issues. Multi-tissue blocks were...

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Serial analysis of gene expression

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a wide variety of organisms. The general goal of the technique is similar to the DNA microarray. However, SAGE sampling is based on sequencing mRNA output...

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Kellie Archer

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Jo Archer (born 1969) is a biostatistician specializing in microarray analysis techniques. She is a professor of biostatistics and chair of the biostatistics...

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Principal component analysis

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Principal component analysis (PCA) is a linear dimensionality reduction technique with applications in exploratory data analysis, visualization and data...

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Transcriptomics technologies

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discipline in biological sciences. There are two key contemporary techniques in the field: microarrays, which quantify a set of predetermined sequences, and RNA-Seq...

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Comparative genomic hybridization

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further explored by other cytological techniques. Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH...

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Reverse phase protein lysate microarray

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A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression...

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Biochip

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differentiated. Microarrays are not limited to DNA analysis; protein microarrays, antibody microarray, chemical compound microarray can also be produced...

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Cluster analysis

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sequence tags (ESTs) or DNA microarrays can be a powerful tool for genome annotation – a general aspect of genomics. Sequence analysis Sequence clustering is...

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Molecular diagnostics

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detection of the marker might use real time PCR, direct sequencing,: ch 17  microarray chips—prefabricated chips that test many markers at once,: ch 24  or MALDI-TOF...

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Proteomics

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number of techniques allow to test for proteins produced during a particular disease, which helps to diagnose the disease quickly. Techniques include western...

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Representational difference analysis

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enhanced through the development of representation oligonucleotide microarray analysis (ROMA), which uses array technology to perform such analyses. This...

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Genetic analysis

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genes. This can be detected on the microarray. Since an array can contain tens of thousands of probes, a microarray experiment can accomplish many genetic...

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Factor analysis

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Public Administration Program Factor Analysis at 100 — conference material FARMS — Factor Analysis for Robust Microarray Summarization, an R package...

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RNA immunoprecipitation chip

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chip) is a molecular biology technique which combines RNA immunoprecipitation with a microarray. The purpose of this technique is to identify which RNA sequences...

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Molecular biology

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PMID 23066278. Tarca AL, Romero R, Draghici S (August 2006). "Analysis of microarray experiments of gene expression profiling". American Journal of Obstetrics...

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Gene set enrichment analysis

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associated with diseases, DNA microarrays were used to measure the amount of gene expression in different cells. Microarrays on thousands of different genes...

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Northern blot

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V., Rice, D. (1999) A comparison of gel-based, nylon filter and microarray techniques to detect differential RNA expression in plants. Current Opinion...

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Text mining

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lexical analysis to study word frequency distributions, pattern recognition, tagging/annotation, information extraction, data mining techniques including...

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Bioinformatics

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mRNA levels with multiple techniques including microarrays, expressed cDNA sequence tag (EST) sequencing, serial analysis of gene expression (SAGE) tag...

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