RIP-chip (RNA immunoprecipitation chip) is a molecular biology technique which combines RNA immunoprecipitation with a microarray. The purpose of this technique is to identify which RNA sequences interact with a particular RNA binding protein of interest in vivo.[1][2][3][4] It can also be used to determine relative levels of gene expression, to identify subsets of RNAs which may be co-regulated, or to identify RNAs that may have related functions.[4][5] This technique provides insight into the post-transcriptional gene regulation which occurs between RNA and RNA binding proteins.[5]
An overview of the major stages in the RNA Immunoprecipitation chip procedure.
^Gagliardi, Miriam; Matarazzo, Maria R. (2016), Lanzuolo, Chiara; Bodega, Beatrice (eds.), "RIP: RNA Immunoprecipitation", Polycomb Group Proteins: Methods and Protocols, Methods in Molecular Biology, vol. 1480, New York, NY: Springer, pp. 73–86, doi:10.1007/978-1-4939-6380-5_7, ISBN 978-1-4939-6380-5, PMID 27659976, retrieved 2020-12-01
^Townley-Tilson, W. H. Davin; Pendergrass, Sarah A.; Marzluff, William F.; Whitfield, Michael L. (2006-10-01). "Genome-wide analysis of mRNAs bound to the histone stem–loop binding protein". RNA. 12 (10): 1853–1867. doi:10.1261/rna.76006. ISSN 1355-8382. PMC 1581977. PMID 16931877.
^Khalil, Ahmad M.; Guttman, Mitchell; Huarte, Maite; Garber, Manuel; Raj, Arjun; Morales, Dianali Rivea; Thomas, Kelly; Presser, Aviva; Bernstein, Bradley E.; Oudenaarden, Alexander van; Regev, Aviv (2009-07-14). "Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression". Proceedings of the National Academy of Sciences. 106 (28): 11667–11672. Bibcode:2009PNAS..10611667K. doi:10.1073/pnas.0904715106. ISSN 0027-8424. PMC 2704857. PMID 19571010.
^ abHendrickson, David G.; Hogan, Daniel J.; Herschlag, Daniel; Ferrell, James E.; Brown, Patrick O. (2008-05-07). "Systematic Identification of mRNAs Recruited to Argonaute 2 by Specific microRNAs and Corresponding Changes in Transcript Abundance". PLOS ONE. 3 (5): e2126. Bibcode:2008PLoSO...3.2126H. doi:10.1371/journal.pone.0002126. ISSN 1932-6203. PMC 2330160. PMID 18461144.
^ abJayaseelan, Sabarinath; Doyle, Francis; Tenenbaum, Scott A. (2014-05-01). "Profiling post-transcriptionally networked mRNA subsets using RIP-Chip and RIP-Seq". Methods. Genomic approaches for studying transcriptional and post-transcriptional processes. 67 (1): 13–19. doi:10.1016/j.ymeth.2013.11.001. ISSN 1046-2023. PMC 4004666. PMID 24257445.
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