Mad2 (mitotic arrest deficient 2) is an essential spindle checkpoint protein. The spindle checkpoint system is a regulatory system that restrains progression through the metaphase-to-anaphase transition. The Mad2 gene was first identified in the yeast S. cerevisiae in a screen for genes which when mutated would confer sensitivity to microtubule poisons.[1] The human orthologues of Mad2 (MAD2L1 and MAD2L2) were first cloned in a search for human cDNAs that would rescue the microtubule poison-sensitivity of a yeast strain in which a kinetochore binding protein was missing.[2] The protein was shown to be present at unattached kinetochores and antibody inhibition studies demonstrated it was essential to execute a block in the metaphase-to-anaphase transition in response to the microtubule poison nocodazole.[2] Subsequent cloning of the Xenopus laevis orthologue, facilitated by the sharing of the human sequence, allowed for the characterization of the mitotic checkpoint in egg extracts.[3]
^Li R, Murray AW (1991). "Feedback control of mitosis in budding yeast". Cell. 66 (3): 519–31. doi:10.1016/0092-8674(81)90015-5. PMID 1651172. S2CID 11306198.
^ abLi Y, Benezra R (1996). "Identification of a human mitotic checkpoint gene: hsMAD2". Science. 274 (5285): 246–8. Bibcode:1996Sci...274..246L. doi:10.1126/science.274.5285.246. PMID 8824189. S2CID 41334564.
^Chen RH, Waters JC, Salmon ED, Murray AW (October 1996). "Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores". Science. 274 (5285): 242–6. Bibcode:1996Sci...274..242C. doi:10.1126/science.274.5285.242. PMID 8824188. S2CID 20142555.
Mad2 (mitotic arrest deficient 2) is an essential spindle checkpoint protein. The spindle checkpoint system is a regulatory system that restrains progression...
conformer of Mad2 (O-Mad2) to the kinetochores. This O-Mad2 changes its conformation to closed Mad2 (C-Mad2) and binds Mad1. This Mad1/C-Mad2 complex is responsible...
phosphorylation sites, which play regulatory roles, are the C-box, the KEN-box, the Mad2-interacting motif, and the Cry box. The KEN-box, as well as the Cry box,...
generate forces that move chromosomes during mitosis. Other proteins, such as Mad2, monitor the microtubule attachment as well as the tension between sister...
depolymerization. Mad1 recruits the anaphase inhibitor Mad2 to unattached kinetochores and is essential for Mad2-Cdc20 complex formation in vivo but not in vitro...
metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. MAD2L1 has been shown to interact...
and Mad2. Upon spindle damage Bub1 is also triggered to phosphorylate Mad1 leading to dissociation of the Mad1-Mad2 complex and thereby rendering Mad2 accessible...
the 1st and 2nd of July, 2012. The theme of the symposium was "appetite." MAD2 Speakers: Tor Nørretranders — Writer, Denmark Roderick Sloan — Sea Urchin...
to TRIP13 is Mad2, which has two forms (open O-Mad2 and closed C-Mad2) (2). When kinetochores are unattached, O-Mad2 converts to C-Mad2, which is then...
gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that...
In molecular biology, the HORMA domain (named after the Hop1p, Rev7p and MAD2 proteins) is a protein domain that has been suggested to recognise chromatin...
Faretta, M; Salmon, ED; Musacchio, A (Feb 8, 2005). "The Mad1/Mad2 complex as a template for Mad2 activation in the spindle assembly checkpoint". Current Biology...
PMID 8626379. O'Neill TJ, Zhu Y, Gustafson TA (April 1997). "Interaction of MAD2 with the carboxyl terminus of the insulin receptor but not with the IGFIR...
APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2". The Journal of Cell Biology. 154 (5): 925–936. doi:10.1083/jcb.200102093...
Location Opened Capacity (million m3/year) Notes Casablanca 2030 250 Between MAD2 and MAD6 per m3. Agadir - Sous Massa 2020 275 World's largest desaliniation...
(TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta". Biochem. J. 343...
zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2". J. Biol. Chem. 275 (6): 4391–4397. doi:10.1074/jbc.275.6.4391. PMID 10660610...
(TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta". The Biochemical...
protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of...
interacting protein of 200 kDa (FIP200 or RB1CC1) and the HORMA (Hop/Rev7/Mad2) domain-containing proteins ATG13 and ATG101. ULK1, specifically, appears...
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