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Immunoprecipitation information


Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.

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Immunoprecipitation

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Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular...

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Chromatin immunoprecipitation

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Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and...

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Immunology

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disease Hypersensitivity Immune disorder Immunodeficiency Significant tests Agglutination Immunoassay Immunoprecipitation Serology Specialist Immunologist...

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RNA immunoprecipitation chip

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RIP-chip (RNA immunoprecipitation chip) is a molecular biology technique which combines RNA immunoprecipitation with a microarray. The purpose of this...

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Clip

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Corticotropin-like intermediate peptide, an endogenous neuropeptide Cross-linking immunoprecipitation, a method used in molecular biology to locate RNA modifications among...

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Methylated DNA immunoprecipitation

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Methylated DNA immunoprecipitation (MeDIP or mDIP) is a large-scale (chromosome- or genome-wide) purification technique in molecular biology that is used...

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Phosphoproteomics

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substrate of any tyrosine kinase. Only by digesting the samples before immunoprecipitation can isolation of only phosphoproteins and temporal profiles of individual...

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Ligand binding assay

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angle position detector. The liquid-phase ligand binding assay of immunoprecipitation (IP) is a method that is used to purify or enrich a specific protein...

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Antibody

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proteins. They are also used in immunoprecipitation to separate proteins and anything bound to them (co-immunoprecipitation) from other molecules in a cell...

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ChIP sequencing

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analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding...

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Chromosome conformation capture

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territories were discovered. In 1984, John T. Lis innovated the Chromatin immunoprecipitation technique. In 1993, the Nuclear Ligation Assay was published, a method...

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Reverse engineering

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specific gene, a regulatory connection can be hypothesized. Chromatin ImmunoPrecipitation (ChIP) methods investigate the genome-wide profile of DNA binding...

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Staphylococcus aureus

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Sengupta M, Jain V, Wilkinson BJ, Jayaswal RK (June 2012). "Chromatin immunoprecipitation identifies genes under direct VraSR regulation in Staphylococcus...

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Epigenetics

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understanding of epigenetic phenomena. These techniques include chromatin immunoprecipitation (together with its large-scale variants ChIP-on-chip and ChIP-Seq)...

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Candida albicans

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Wor1 control of each gene, based on Wor1 enrichment in chromatin immunoprecipitation experiments. Activation (arrowhead) and repression (bar) are inferred...

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Adipose tissue

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as a potential therapeutic molecule to induce beiging. Chromatin immunoprecipitation with sequencing (ChIP-seq) is a method used to identify protein binding...

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Coprecipitation

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employed. Analogously, in medicine, coprecipitation (referred to as immunoprecipitation) is specifically "an assay designed to purify a single antigen from...

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Paraformaldehyde

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also used to crosslink proteins to DNA, as used in ChIP (chromatin immunoprecipitation) which is a technique to determine which part of DNA certain proteins...

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Endogenous retrovirus

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the p53 pathway, which results in cell apoptosis. Using chromatin immunoprecipitation with sequencing, thirty-percent of all p53-binding sites were located...

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Lysis buffer

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Adjust pH to 7.4 RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer...

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Hybridoma technology

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immunocytochemical, western blot, and immunoprecipitation-mass spectrometry. Unlike western blot assays, immunoprecipitation-mass spectrometry facilitates screening...

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Laminin

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and its regulation during EMT were confirmed by ribonucleoprotein immunoprecipitation. La is able to positively modulate LamB1 IRES translation, so LamB1...

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Monoclonal antibody

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purify their target compounds from mixtures, using the method of immunoprecipitation. Therapeutic monoclonal antibodies act through multiple mechanisms...

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Cellular differentiation

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genomic binding site or not. This can be determined using a chromatin immunoprecipitation (ChIP) assay. DNA-nucleosome interactions are characterized by two...

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IP

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County, Romania IP Casino Resort Spa, in Biloxi, Mississippi, US Immunoprecipitation, a molecular biology technique Incontinentia pigmenti, a genetic...

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Sodium channel

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also known as F3 or F11, associates with beta 1 as shown via co-immunoprecipitation. Fibronectin-like (FN-like) repeats of Tenascin-C and Tenascin-R...

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