Scheme of isoelectric focusing with immobilized pH gradient (IPG) gels.
Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI).[1][2] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the pH of its surroundings.[3]
^Bjellqvist, Bengt; Ek, Kristina; Righetti, Pier Giorgio; Gianazza, Elisabetta; Görg, Angelika; Westermeier, Reiner; Postel, Wilhelm (1982). "Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications". Journal of Biochemical and Biophysical Methods. 6 (4): 317–339. doi:10.1016/0165-022X(82)90013-6. ISSN 0165-022X. PMID 7142660.
^Pier Giorgio Righetti (1 April 2000). Isoelectric Focusing: Theory, Methodology and Application. Elsevier. ISBN 978-0-08-085880-7.
^David Edward Garfin (1990). Isoelectric focusing. Methods in Enzymology. Vol. 182. pp. 459–77. doi:10.1016/0076-6879(90)82037-3. ISBN 9780121820831. PMID 2314254.
and 21 Related for: Isoelectric focusing information
Isoelectricfocusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point...
constant pH to separate proteins, or isoelectricfocusing, which uses a pH gradient to separate proteins. Isoelectricfocusing is also the first step in 2-D...
Isoelectric may refer to: Isoelectric point, the pH at which a particular molecule carries no net electrical charge Isoelectricfocusing, a technique for...
known as the molecule's isoelectric point. Ampholytes are used to establish a stable pH gradient for use in isoelectricfocusing. Metal oxides which react...
electrophoresis)/Coomassie blue staining", and (b) the combination of isoelectricfocusing/silver staining. The latter is more sensitive. For the analysis of...
separation, an additional separation method may then be used, such as isoelectricfocusing or SDS-PAGE. The gel will then be physically cut, and the protein...
components of agar. The proteins may be separated by charge and/or size (isoelectricfocusing agarose electrophoresis is essentially size independent), and the...
have different isoelectric points. This permits a technique called isoelectricfocusing, which is used for separation of proteins by 2-D gel polyacrylamide...
techniques including capillary gel electrophoresis (CGE), capillary isoelectricfocusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography...
alternatives to conventional hemoglobin electrophoresis include isoelectricfocusing, capillary zone electrophoresis, and high-performance liquid chromatography...
electrophoresis History of gel electrophoresis Immunoelectrophoresis Isoelectricfocusing Isotachophoresis Nonlinear frictiophoresis Pulsed-field gel electrophoresis...
electrophoresis. In the first dimension, the proteins are separated by isoelectricfocusing, which resolves proteins on the basis of charge. In the second dimension...
prolamins and are separated on the basis of electrophoretic mobility and isoelectricfocusing. Gliadin peptides cross the intestinal barrier by active transport...
"Characterization of human exocrine pancreatic proteins by two-dimensional isoelectricfocusing/sodium dodecyl sulfate gel electrophoresis". Gastroenterology. 80...
electrophoresis. The three samples are mixed and loaded onto IEF (isoelectricfocusing chromatography) for first dimension and the strip is transferred...
evidence of plasma cell dyscrasias. ATTR can be identified using isoelectricfocusing which separates mutated forms of transthyretin. Findings can be corroborated...
(2008). "Fractionation of Complex Protein Mixtures by Liquid-Phase IsoelectricFocusing". 2D PAGE: Sample Preparation and Fractionation. Methods in Molecular...
anodizing, electrowinning – depositing a material on an electrode Isoelectricfocusing a.k.a. electrofocusing – similar to electroplating, but separating...
IPG may refer to: Immobilized pH gradient, a method used in isoelectricfocusing Impedance phlebography, a medical test Implanted pulse generator (neurostimulator)...
"Cerebrospinal Fluid Oligoclonal Bands in the Diagnosis of Multiple Sclerosis IsoelectricFocusing With IgG Immunoblotting Compared With High-Resolution Agarose Gel...
variation in different varieties of Siamese fighting fish using isoelectricfocusing of sarcoplasmic proteins". Aquaculture International. 5 (6): 537–549...
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