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Epitope binning is a competitive immunoassay used to characterize and then sort a library of monoclonal antibodies against a target protein.[1] Antibodies against a similar target are tested against all other antibodies in the library in a pairwise fashion to see if antibodies block one another's binding to the epitope of an antigen.[2] After each antibody has a profile created against all of the other antibodies in the library, a competitive blocking profile is created for each antibody relative to the others in the library. Closely related binning profiles indicate that the antibodies have the same or a closely related epitope and are "binned" together.[2] Epitope binning is referenced in the literature under different names such as epitope mapping and epitope characterization.[2] Regardless of the naming, epitope binning is prevalent in the pharmaceutical industry.[2] Epitope Binning is used in the discovery and development of new therapeutics, vaccines, and diagnostics.[3]
^Abdiche, Y. N.; D. S. Malashock; A. Pinkerton; J. Pons (March 2009). "Exploring blocking assays using Octet, ProteOn, and Biacore biosensors". Analytical Biochemistry. 386 (2): 172–180. doi:10.1016/j.ab.2008.11.038. PMID 19111520.
^ abcdBrooks, B.D. (2014). "The Importance of Epitope Binning in Drug Discovery". Current Drug Discovery Technologies. 11 (2): 109–12. doi:10.2174/1570163810666131124233827. PMC 4033553. PMID 24266537.
^Estep, P.; Reid F; Nauman C; Liu Y; Sun T; Sun J; Xu Y. (2013). "High throughput solution-based measurement of antibody-antigen affinity and epitope binning". mAbs. 5 (2): 270–278. doi:10.4161/mabs.23049. PMC 3893237. PMID 23575269.
Epitopebinning is a competitive immunoassay used to characterize and then sort a library of monoclonal antibodies against a target protein. Antibodies...
requires separate, specific antibodies.[citation needed] Cryptotope Epitopebinning Mimotope Odotope Polyclonal B cell response Protein tag TimeSTAMP protein...
In immunology, epitope mapping is the process of experimentally identifying the binding site, or epitope, of an antibody on its target antigen (usually...
configuration. Additionally, it allows the mapping of epitopes as antibodies of overlapping epitopes will be associated with an attenuated signal compared...
is reversed and proteins are removed by digestion with proteinase K. An epitope-tagged version of the protein of interest, or in vivo biotinylation can...
strands during PCR. The binding does not depend on a specific sequence or epitope on collagen, enabling CHPs to target denatured collagen chains of different...
Grötzinger J (January 2004). "Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy". The Journal of Biological...
Fussell H, Thomas M, Street J, Darke C (1996). "HLA-A9 antibodies and epitopes". Tissue Antigens. 47 (4): 307–12. doi:10.1111/j.1399-0039.1996.tb02558...
of 38 cm. Antimicrobial compounds can be found in Narcine timlei. The epitopes of N.timlei have remained remarkably conserved throughout evolution. This...
utilization as an anticancer vaccine, inducing antibody response against the epitope. The resulting humoral immunity could enable the selective eradication...
9 and D1.34 (PDB: 2V5R, 2V5S) shows large variations in their binding epitopes and dimerization interface and conformations. Much of the difference is...
W.; Cai, Fangping (January 2013). "Vertical T cell immunodominance and epitope entropy determine HIV-1 escape". The Journal of Clinical Investigation...
"SARS-CoV-2 variants of concern: spike protein mutational analysis and epitope for broad neutralization". Nature Communications. 13 (1): 4696. Bibcode:2022NatCo...
for laying down the m5C mark. First, the cell is made to overexpress an epitope-tagged m5C-RNA methytransferase derivative so that the antibody used later...
"SARS-CoV-2 variants of concern: spike protein mutational analysis and epitope for broad neutralization". Nature Communications. 13 (1): 4696. Bibcode:2022NatCo...
fsTnT is linked with fsTnI genes (Fig. 1). Sequence homology and protein epitope allosteric similarity data suggest that TnT gene was originated by duplication...
(fsTnI)-fsTnT, ssTnI-cardiac (cTnT) and cTnI-ssTnT gene pairs. Sequence and epitope conservation studies suggested that genes encoding the muscle type specific...
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