Schematic of a CHP strand (labeled with an "X" tag) hybridizing to denatured collagen chains and forming a collagen triple helix. During disease progression, tissue development, or ageing, collagen can be extensively degraded by collagenolytic proteases, causing its triple helix to unfold at the physiological temperature due to reduced thermal stability. X may represent a biotin or fluorescent tag.
A collagen hybridizing peptide (CHP) is a synthetic peptide sequence with typically 6 to 10 repeating units of the Gly-Xaa-Yaa amino acid triplet, which mimics the hallmark sequence of natural collagens.[1][2] A CHP peptide usually possesses a high content of proline and hydroxyproline in the Xaa and Yaa positions, which confers it a strong propensity to form the collagen's unique triple helix conformation.[1][3] In the single-stranded (monomeric) status, the peptide can recognize denatured collagen strands in tissues by forming a hybridized triple helix with the collagen strands.[2] This occurs via the triple helical chain assembly and inter-chain hydrogen bonding, in a manner similar to primers binding to melted DNA strands during PCR.[4] The binding does not depend on a specific sequence or epitope on collagen, enabling CHPs to target denatured collagen chains of different types.[5][6]
^ abShoulders, Matthew D.; Raines, Ronald T. (2009). "Collagen structure and stability". Annual Review of Biochemistry. 78: 929–958. doi:10.1146/annurev.biochem.77.032207.120833. ISSN 1545-4509. PMC 2846778. PMID 19344236.
^ abWahyudi, Hendra; Reynolds, Amanda A.; Li, Yang; Owen, Shawn C.; Yu, S. Michael (October 2016). "Targeting collagen for diagnostic imaging and therapeutic delivery". Journal of Controlled Release. 240: 323–331. doi:10.1016/j.jconrel.2016.01.007. PMC 4936964. PMID 26773768.
^Persikov, A. V.; Ramshaw, J. A.; Kirkpatrick, A.; Brodsky, B. (2000-12-05). "Amino acid propensities for the collagen triple-helix". Biochemistry. 39 (48): 14960–14967. doi:10.1021/bi001560d. ISSN 0006-2960. PMID 11101312.
^Li, Yang; Yu, S. Michael (December 2013). "Targeting and mimicking collagens via triple helical peptide assembly". Current Opinion in Chemical Biology. 17 (6): 968–975. doi:10.1016/j.cbpa.2013.10.018. ISSN 1879-0402. PMC 3863647. PMID 24210894.
^Hwang, Jeongmin; Huang, Yufeng; Burwell, Timothy J.; Peterson, Norman C.; Connor, Jane; Weiss, Stephen J.; Yu, S. Michael; Li, Yang (2017-10-24). "In Situ Imaging of Tissue Remodeling with Collagen Hybridizing Peptides". ACS Nano. 11 (10): 9825–9835. doi:10.1021/acsnano.7b03150. ISSN 1936-0851. PMC 5656977. PMID 28877431.
^Li, Yang; Ho, Daniel; Meng, Huan; Chan, Tania R.; An, Bo; Yu, Hanry; Brodsky, Barbara; Jun, Albert S.; Michael Yu, S. (2013-01-16). "Direct Detection of Collagenous Proteins by Fluorescently Labeled Collagen Mimetic Peptides". Bioconjugate Chemistry. 24 (1): 9–16. doi:10.1021/bc3005842. ISSN 1043-1802. PMC 3586774. PMID 23253177.
and 21 Related for: Collagen hybridizing peptide information
A collagenhybridizingpeptide (CHP) is a synthetic peptide sequence with typically 6 to 10 repeating units of the Gly-Xaa-Yaa amino acid triplet, which...
a collagen hybridizingpeptide is specifically hybridized to the degraded, unfolded collagen chains, can be used to image degraded collagen and inform...
pentameric arrangement termed microfibril. Collagenhybridizingpeptide, a peptide that can bind to denatured collagen Hypermobility spectrum disorder Metalloprotease...
related to Peptide. Acetyl hexapeptide-3 Beefy meaty peptideCollagenhybridizingpeptide, a short peptide that can bind to denatured collagen in tissues...
the middle of the cell). CollagenHybridizingPeptide (CHP) staining allows for an easy, direct way to stain denatured collagens of any type (Type I, II...
yellow Collagen fibers in red Muscular fibers in yellow Red blood cells in yellow CollagenHybridizingPeptide, a peptide that stains denatured collagen in...
cells. A new peptide, known as the CollagenHybridizingPeptide, can also be conjugated with fluorophores and used to stain denatured collagen fibers. Staining...
their activity in animal models. Collagenhybridizingpeptide, a peptide that can bind and stain MMP cleaved collagen Drug discovery and development of...
pathological, characterized by excessive inflammation and fibrosis. Collagenhybridizingpeptide, a molecular marker to directly image tissue remodeling Lee BC...
Fibronectin also binds to other extracellular matrix proteins such as collagen, fibrin, and heparan sulfate proteoglycans (e.g. syndecans). Fibronectin...
interactions. This protein can bind to fibrinogen, fibronectin, laminin, collagens types V and VII and integrins alpha-V/beta-1. This protein has been shown...
proteins from the Sea lamprey Petromyzon marinus. Lamprin, an insoluble non-collagen, non-elastin protein, is the major connective tissue component of the fibrillar...
into the impact of Praziquantel use on systemic OPN levels and on liver collagen deposition in chronic murine schistosomiasis revealed that Praziquantel...
potential transmembrane domain and a signal peptide (Figure 2). The SignalP-5.0 prediction for the signal peptide reported a likelihood of 0.9989 with a cleavage...
promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin...
demonstrating that digestion of the peptide with either heparanase or chondroitinase did not lead to complete loss of the peptide's activity, it was shown that...
encodes the transmembrane domain (amino acid position 31-49) and no signal peptide. TNMD contains a putative protease recognition sequence (Arg-Xxx-Xxx-Arg)...
Global Information