Typical dot blot membrane. Darker dots indicate more protein.
A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. Instead, the sample is applied directly on a membrane in a single spot, and the blotting procedure is performed.
The technique offers significant savings in time, as chromatography or gel electrophoresis, and the complex blotting procedures for the gel are not required. However, it offers no information on the size of the target protein.[1]
^"Test Blots, Slot Blots & Dot Blots - Immunodetection | Bio-Rad". Bio-Rad.
A dotblot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with...
specific target protein. Other related techniques include dotblot analysis, quantitative dotblot, immunohistochemistry and immunocytochemistry, where antibodies...
oligonucleotide (ASO) probes, a process that can often be done by a simple dotblot. Amplified fragment length polymorphism (AFLP) RAPD STR analysis Saiki...
antigen (with a test such as ELISA or antigen microarray assay) or immuno-dotblot. The most productive and stable clone is then selected for future use....
chemically modified paper filters or microporous membrane filters. In a dotblot, macromolecules are applied directly to the matrix. Macromolecules can...
functionally mapping epitopes often use binding assays such as western blot, dotblot, and/or ELISA to determine antibody binding. Competition methods look...
spectrometry (MS). However, a recent developed method of quantitative dotblot (QDB) analysis is able to measure both the absolute and relative quantity...
Allium L. subgenus Rhizirideum (G. Don ex Koch) Wendelbo according to dotblot hybridization with randomly amplified DNA probes". Theoretical and Applied...
antigen-antibody reactions are being explored, such as immuno-PCR. A rapid dot-blot ELISA test is also available for quick, effective, and economical on-site...
oligonucleotide (ASO) probes, a process that can often be done by a simple dotblot. RFLP https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechCAPS.shtml...
nylon membranes, which can be accomplished by use of dotblots or bidirectional agarose gel blotting and UV fixation of the DNA to the membranes. In many...
analyzed, either a very specific antibody needs to be used in quantitative dotblot analysis (QDB), or biochemical separation then needs to be used before...
rabbit antisera against representative strains with diverse origins and by Dotblot assays. These results endorsed the establishment of two different groups...
Enzymology vol. 155(F) pp. 335–50 (1987). Verlaan-de Vries M et al. "A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides...
protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large...
measured by the cccDNA levels via Southern blot kinetics of healthy vs infected cells and quantified by dotblot. In these infected cells, there is a strong...
circovirus by using polymerase chain reaction (PCR)-based methods and dotblot hybridisation (DBH) tests. Infection with DuCV appears to cause growth...
health screening study on 35,365 people, using a sensitive chemiluminescent dotblot assay". Sensors. 11 (12): 11064–80. Bibcode:2011Senso..1111064C. doi:10...
stationary phase Reverse phase protein lysate microarray, a micro-cell lysate dot-blot that allows measurement of protein expression levels This disambiguation...
increased sensitivity compared to methods such as RNase protection assays and dotblot hybridization. The disadvantage to RT-PCR is the requirement of costly...
identify avian reoviruses molecularly by observing infected tissues with dot-blot hybridization, PCR, and a combination of PCR and RFLP. This combination...
Allium L. subgenus Rhizirideum (G. Don ex Koch) Wendelbo according to dotblot hybridization with randomly amplified DNA probes". Theoretical and Applied...
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