Deoxyribonuclease I (usually called DNase I), is an endonuclease of the DNase family coded by the human gene DNASE1.[5]
DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.[6]
DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However, since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner.
At least six autosomal codominant alleles of the gene DNASE 1 have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene, as well as factor inactivating its enzyme product, have been associated with systemic lupus erythematosus (SLE), an autoimmune disease.[7][8] A recombinant form of this protein is used to treat one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity.[9] Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.[5]
^ abcGRCh38: Ensembl release 89: ENSG00000213918 – Ensembl, May 2017
^ abcGRCm38: Ensembl release 89: ENSMUSG00000005980 – Ensembl, May 2017
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ab"Entrez Gene: DNASE1 deoxyribonuclease I".
^Samejima, K. & Earnshaw, W.C. (2005). "Trashing the genome: the role of nucleases during apoptosis". Nat Rev Mol Cell Biol. 6 (9): 677–88. doi:10.1038/nrm1715. PMID 16103871. S2CID 13948545.
^Hakkim A, Fürnrohr BG, Amann K, Laube B, Abed UA, Brinkmann V, Herrmann M, Voll RE, Zychlinsky A (2010). "Impairment of neutrophil extracellular trap degradation is associated with lupus nephritis". Proc Natl Acad Sci U S A. 107 (21): 9813–8. Bibcode:2010PNAS..107.9813H. doi:10.1073/pnas.0909927107. PMC 2906830. PMID 20439745.
^Yasutomo K, Horiuchi T, Kagami S, et al. (2001). "Mutation of DNASE1 in people with systemic lupus erythematosus". Nat. Genet. 28 (4): 313–4. doi:10.1038/91070. PMID 11479590. S2CID 21277651.
^Shak S, Capon DJ, Hellmiss R, et al. (1991). "Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum". Proc. Natl. Acad. Sci. U.S.A. 87 (23): 9188–92. doi:10.1073/pnas.87.23.9188. PMC 55129. PMID 2251263.
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DeoxyribonucleaseI (usually called DNase I), is an endonuclease of the DNase family coded by the human gene DNASE1. DNase I is a nuclease that cleaves...
main types of DNase found in animals are known as deoxyribonucleaseI (DNase I) and deoxyribonuclease II (DNase II). These two families have subcategories...
within a polynucleotide chain (namely DNA or RNA). Some, such as deoxyribonucleaseI, cut DNA relatively nonspecifically (without regard to sequence)...
Deoxyribonuclease gamma is an enzyme that in humans is encoded by the DNASE1L3 gene. This gene encodes a member of the DNase family. The protein hydrolyzes...
encodes a member of the deoxyribonuclease family and the protein and DNA shows high sequence similarity to lysosomal DNase I. Alternate transcriptional...
Deoxyribonuclease IV (phage-T4-induced) (EC 3.1.21.2, endodeoxyribonuclease IV (phage T4-induced), E. coli endonuclease IV, endodeoxyribonuclease, redoxyendonuclease...
variable number of tandem repeat polymorphisms within the human deoxyribonucleaseI gene". Legal Medicine. 17 (4): 283–6. doi:10.1016/j.legalmed.2015...
Deoxyribonuclease-1-like 2 is an enzyme that in humans is encoded by the DNASE1L2 gene. GRCh38: Ensembl release 89: ENSG00000167968 – Ensembl, May 2017...
restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix. These enzymes are found in bacteria...
while the R represents the particular strain, in this case RY13, and the I denotes that it was the first enzyme isolated from this strain.[citation needed]...
single-stranded substrate. Laskowski M (1966). ", Sr. Pancreatic deoxyribonucleaseI". In Cantoni GL, Davies DR (eds.). Procedures in Nucleic Acid Research...
binding site on a particular DNA molecule. The method uses an enzyme, deoxyribonuclease (DNase, for short), to cut the radioactively end-labeled DNA, followed...
signals exist. The variant with the longest 3' UTR overlaps the deoxyribonucleaseI-like 1 gene on the opposite strand. This gene is co-transcribed with...
Bibcode:1995Sci...269..656N. doi:10.1126/science.7624794. PMID 7624794. Deoxyribonuclease+BamHI at the U.S. National Library of Medicine Medical Subject Headings...
DNA. Kato M, Ikeda Y (1968). "On the deoxyribonucleases, K1 and K2, isolated from mycelia of Aspergillus oryzae. I. Isolation and purification of DNases...
exonuclease V, E. coli exonuclease V, gene recBC endoenzyme, RecBC deoxyribonuclease, gene recBC DNase, gene recBCD enzymes) is an enzyme of E. coli that...
tracheobronchoplasty, and tracheostomy. The role of the nebulised recombinant human deoxyribonuclease (rhDNase) remains inconclusive. Tracheal collapse for the condition...
like hyaluronidase, lipase, collagenase, elastase, ribonuclease, deoxyribonuclease also play an important role in preventing the spread of infection...
from mannitol + Acid from sucrose + Nitrate reduction + (to nitrite) Deoxyribonuclease, 25 °C + Lipase + Pigment some biovars produce red Catalase production...
same reason in the débridement of infected wounds. Hyaluronidase, deoxyribonuclease, lecithinase, leukocidin, protease, lipase, and hemolysin are also...
chemical mediators, such as eosinophil peroxidase, ribonuclease (RNase), deoxyribonucleases (DNase), lipase, plasminogen, and major basic protein. These mediators...
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