Experimental tools for evaluation of chemotactic ability of cells
The process of chemotaxis can be demonstrated using a capillary tube assay (shown above). The motile prokaryotes can sense chemicals in their environment and change their motility accordingly. When no chemicals are present, movement is completely random. When a repellent or attractant chemical is present, the motility changes; runs become longer and tumbles become less frequent so that the net movement towards or away from the chemical can be achieved. The net movement can be seen in the beaker, where the bacteria accumulate around the attractant, and away from the repellent. The beaker used in this illustration is from Wikimedia Commons - "Laboratory Glassware - Beaker" by Amanda44.
Chemotaxis assays are experimental tools for evaluation of chemotactic ability of prokaryotic or eukaryotic cells.
A wide variety of techniques have been developed. Some techniques are qualitative - allowing an investigator to approximately determine a cell's chemotactic affinity for an analyte - while others are quantitative, allowing a precise measurement of this affinity.
Chemotaxisassays are experimental tools for evaluation of chemotactic ability of prokaryotic or eukaryotic cells. A wide variety of techniques have been...
recognized that mechanisms that allow chemotaxis in animals can be subverted during cancer metastasis. The aberrant chemotaxis of leukocytes and lymphocytes also...
Migration assays, Chemotaxisassay Secretion assays Apoptosis assays such as the DNA laddering assay, the Nicoletti assay, caspase activity assays, and Annexin...
and not useful for non-adherent cells. Specifically, this assay isn't ideal for chemotaxis studies. This laboratory technique has various advantages. ...
inventor 4301 Boyden, main-belt asteroid Boyden Chamber, a type of chemotaxisassay This disambiguation page lists articles associated with the title Boyden...
guidance in the form of sperm chemotaxis has been established in a large variety of species Although sperm chemotaxis is prevalent throughout the Metazoa...
feature of the chemotaxis receptors and ligands provides the possibility to select chemotactic responder cells with a simple chemotaxisassay. By chemotactic...
Another setup commonly used for measuring chemotaxis and chemokinesis utilizes the under-agarose cell migration assay, whereby a layer of agarose gel is placed...
invertebrates the guidance is done by chemotaxis. In the case of mammals, it appears to be done by chemotaxis, thermotaxis and rheotaxis. Since the discovery...
dendritic cells and macrophages also helped foster this idea. In vitro chemotaxisassays showed it to be utilized in attracting these cells. As an adipokine...
gliding and twitching motility. Under-agarose cell migration assay may be used to measure chemotaxis and chemokinesis. A layer of agarose gel is placed between...
van Strijp J, Furebring C (23 September 2010). "Directed evolution of chemotaxis inhibitory protein of Staphylococcus aureus generates biologically functional...
be attributed to factors such as Chemotaxis, bacteria's ability to sense and migrate towards nutrients. The Chemotaxis mechanism can be amplified by social...
named "KC" for its location in the nitrocellulose colony hybridization assay. This designation is sometimes erroneously believed to be an acronym and...
reaches the isthmus of the fallopian tube. Various mechanisms, including chemotaxis, aid the sperm in approaching the ovum in the ampulla of the fallopian...
hormone terminology, cytokines may be autocrine or paracrine in nature, and chemotaxis, chemokinesis and endocrine as a pyrogen. Essentially, cytokines are not...
other phagocytes already present. The phagocytes move by a method called chemotaxis. When phagocytes come into contact with bacteria, the receptors on the...
and collagen I induce chemotaxis and haptotaxis of human and rabbit mesenchymal stem cells in a standardized transmembrane assay. Stem cells and development...
surface of ciliated bronchial host cells. This binding may alter cellular chemotaxis and reduce the migration of immune cells into the lungs of infected individuals...
infections. The phagocytic process can be separated into several major stages: chemotaxis (migration of phagocytes to inflammatory sites), attachment of particles...
There are three steps to fertilisation that ensure species-specificity: Chemotaxis Sperm activation/acrosomal reaction Sperm/egg adhesion Consideration as...
chemoattractant gradients makes microfluidics the ideal tool to study motility, chemotaxis and the ability to evolve / develop resistance to antibiotics in small...
angiogenic cell migration in vivo and anti-sense calponin 2 RNA reduced chemotaxis of human umbilical vein endothelial cells in culture. A hypothesis is...
observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. In humans, the IFNG protein is encoded by...
acetylcholine receptors. Experimental assays in animal models are needed to validate a chemically induced chemotaxis by use of anticholinergic drugs to prevent...
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