CEllular Thermal Shift Assay (CETSA®) is a patented label free chemoproteomics method that has enabled measurements of compound target engagement in intact cells and tissue, without modifications to the target protein. This is accomplished by comparing the measured cellular thermal stability of the protein in the presence and absence of the test compound. An efficacious compound binding to its intended target will affect associated proteins and thereby leave traces in the cell in form of changed signalling patterns. Such patterns can arise from for example loss or gain of protein-protein interactions, phosphorylations or release of regulatory molecules.[1][2][3][4][5]
^Molina, Daniel Martinez; Jafari, Rozbeh; Ignatushchenko, Marina; Seki, Takahiro; Larsson, E. Andreas; Dan, Chen; Sreekumar, Lekshmy; Cao, Yihai; Nordlund, Pär (2013-07-05). "Monitoring Drug Target Engagement in Cells and Tissues Using the Cellular Thermal Shift Assay". Science. 341 (6141): 84–87. doi:10.1126/science.1233606. ISSN 0036-8075. PMID 23828940. S2CID 5225262.
^Savitski, Mikhail M.; Reinhard, Friedrich B. M.; Franken, Holger; Werner, Thilo; Savitski, Maria Fälth; Eberhard, Dirk; Molina, Daniel Martinez; Jafari, Rozbeh; Dovega, Rebecca Bakszt; Klaeger, Susan; Kuster, Bernhard (2014-10-03). "Tracking cancer drugs in living cells by thermal profiling of the proteome". Science. 346 (6205). doi:10.1126/science.1255784. hdl:10616/42298. ISSN 0036-8075. PMID 25278616. S2CID 206558838.
^Almqvist, Helena; Axelsson, Hanna; Jafari, Rozbeh; Dan, Chen; Mateus, André; Haraldsson, Martin; Larsson, Andreas; Molina, Daniel Martinez; Artursson, Per; Lundbäck, Thomas; Nordlund, Pär (2016-03-24). "CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil". Nature Communications. 7 (1): 1–11. doi:10.1038/ncomms11040. ISSN 2041-1723. PMC 4820820.
CEllularThermalShiftAssay (CETSA®) is a patented label free chemoproteomics method that has enabled measurements of compound target engagement in intact...
A thermalshiftassay (TSA) measures changes in the thermal denaturation temperature and hence stability of a protein under varying conditions such as...
to as the Mass Spectrometry CellularThermalShiftAssay (MS-CETSA). The Drug Affinity Responsive Target Stability assay follows a similar basic assumption...
Lundbäck T, Nordlund P, Martinez Molina D (September 2014). "The cellularthermalshiftassay for evaluating drug target interactions in cells". Nature Protocols...
if the protein has distinguishable spectroscopic features, or by enzyme assays if the protein has enzymatic activity. Additionally, proteins can be isolated...
molecules in cells behave in radically different ways than in test-tube assays. Consequently, measurements of the properties of enzymes or processes in...
a treatment for diseases that are caused by ecDNA in the blood plasma. Assays of DNase are emerging in the research field as well. The two main types...
by cellular factors to restrict viral replication. Two key processes that influenza viruses evolve through are antigenic drift and antigenic shift. Antigenic...
exhibit remarkable properties, such as exceptional tensile strength and thermal conductivity because of their nanostructure and strength of the bonds between...
Harvard Medical School, both teams competing against each other to find an assay for phosphorus. It was proposed to be the intermediary between energy-yielding...
many RNAs (transcriptome-wide probing) and the direct assay of RNA molecules in their cellular environment (in-cell probing). Structured RNA is first...
antibodies in sensors: 1. The antibody binding capacity is strongly dependent on assay conditions (e.g. pH and temperature), and 2. the antibody-antigen interaction...
Luan P, Vlahos CJ, et al. (March 2005). "A cell-based immunocytockemical assay for monitoring kinase signaling pathways and drug efficacy" (PDF). Anal...
January 2019. In January 2010, hallmarks for palladium were introduced by assay offices in the United Kingdom, and hallmarking became mandatory for all...
fluke infection: Development of an indirect enzyme-linked immunosorbent assay". Fish and Shellfish Immunology. 25 (1–2): 66–75. doi:10.1016/j.fsi.2007...
"Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp". PLOS ONE. 7 (10): e46147. Bibcode:2012PLoSO...746147M. doi:10...
(such as indirect hemagglutination, ELISA (enzyme linked immunosorbent assay), immunoblots or latex agglutination) that use antigens specific for E....
of shifting the resonance wavelengths by tuning the particle geometry allows using them in the fields of molecular labeling, biomolecular assays, trace...
sample is detected on a western blot. By using electrophoretic mobility shiftassay (EMSA), the activation profile of transcription factors can be detected...
substance to a cellular target. One assay to determine the extent at which a ligand binds to its receptor is the radioligand binding assay (RBA), in which...
mechanisms that could confuse results. Various genetic tools, protocols, and assays make Drosophila a classical model for studying the innate immune system...
"sodium-D" light (589 nm) is +66.47°. Commercial samples of sugar are assayed using this parameter. Sucrose does not deteriorate at ambient conditions...
effects for cell structure and function, which may not appear non-toxic in assays but preparation of the particles have been found to produce abnormal effects...
protein for 9-O-acetyl groups on sialic acids have been used in various assays using whole virions. Proteolytic cleavage must occur before any membrane...
science assays are shown below along with their corresponding acceptor dye as well as their excitation and emission wavelengths and resultant Stokes shift (separation...
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