The Illumina Methylation Assay using the Infinium I platform uses 'BeadChip' technology[clarification needed] to generate a comprehensive genome-wide profiling of human DNA methylation. Similar to bisulfite sequencing and pyrosequencing, this method quantifies methylation levels at various loci within the genome. This assay is used for methylation probes on the Illumina Infinium HumanMethylation27 BeadChip (henceforth, 27k [methylation] array). Probes on the 27k array target regions of the human genome to measure methylation levels at 27,578 CpG dinucleotides in 14,495 genes.[1] In 2008, Illumina released the Infinium HumanMethylation450 BeadChip array (“450 K array”), which targets over 450,000 methylation sites.[2] In 2016, the Infinium MethylationEPIC BeadChip ("EPIC") was released, which interrogates over 850,000 methylation sites across the human genome.[3]
^Weisenberger, DJ. et al. (2008) Comprehensive DNA Methylation Analysis on the Illumina Infinium Assay Platform. [1]
The IlluminaMethylationAssay using the Infinium I platform uses 'BeadChip' technology[clarification needed] to generate a comprehensive genome-wide profiling...
binding to DNA incompletely converted by bisulfite. The IlluminaMethylationAssay is one such assay that applies the bisulfite sequencing technology on a...
Differentially Methylated Position; a methylated probe in the IlluminaMethylationAssay Doctor of Medical Physics, a degree in medical physics Dance movement...
earliest methylation detection assays used methylation modification sensitive restriction endonucleases. Genomic DNA was digested with both methylation-sensitive...
mono-methylation at the 36th lysine residue of the histone H3 protein. There are diverse modifications at H3K36, such as phosphorylation, methylation, acetylation...
high-throughput sequencing system developed by biotechnology company, Illumina, perform comprehensive assays based on sequencing-by-synthesis of base pairs. Following...
modification to the DNA packaging protein Histone H3 that indicates tri-methylation at the 4th lysine residue of the histone H3 protein and is often involved...
DNA samples are then assayed on high-throughput technologies such as DNA methylation arrays (e.g., Illumina Infinium MethylationEPIC (850K) array) or...
H3. It is a mark that indicates the tri-methylation of lysine 27 on histone H3 protein. This tri-methylation is associated with the downregulation of...
diagram shows the progressive methylation of a lysine residue. The di-methylation (third from left) denotes the methylation present in H3K36me2. The genomic...
that indicates the di-methylation at the 3rd arginine residue of the histone H4 protein. In epigenetics, arginine methylation of histones H3 and H4 is...
diagram shows the progressive methylation of a lysine residue. The di-methylation (third from left) denotes the methylation present in H3K79me2. The genomic...
This diagram shows the progressive methylation of a lysine residue. The tri-methylation (right) denotes the methylation present in H3K36me3. The genomic...
This diagram shows the progressive methylation of a lysine residue. The tri-methylation (right) denotes the methylation present in H3K9me3 . The genomic...
that indicates the di-methylation at the 2nd arginine residue of the histone H3 protein. In epigenetics, arginine methylation of histones H3 and H4 is...
that indicates the di-methylation at the 17th arginine residue of the histone H3 protein. In epigenetics, arginine methylation of histones H3 and H4 is...
diagram shows the progressive methylation of a lysine residue. The mono-methylation (second from left) denotes the methylation present in H4K20me. H4K20me...
diagram shows the progressive methylation of a lysine residue. The mono-methylation (second from left) denotes the methylation present in H3K4me1. The genomic...
methyltransferase SETDB1 binds to methylated H3 with both K14 acetylation and K9 methylation. SETDB1 silences retroviruses and gene regulation. Proteins are typically...
that indicates the di-methylation at the 8th arginine residue of the histone H3 protein. In epigenetics, arginine methylation of histones H3 and H4 is...
inference of expression levels of individual genes, thereby augmenting the assay's applicability in disease characterization, histological classification...
millions of 200-400bp reads in 2009), and the Solexa system (now owned by Illumina) (introduced in 2006, generating hundreds of millions of 50-100bp reads...
Global Information