An agar plate containing tryptone supporting growth of a micro-organism.
Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.[1]
Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms.[2] It provides a source of amino acids for the growing bacteria. Tryptone is similar to casamino acids, both being digests of casein, but casamino acids can be produced by acid hydrolysis and typically only have free amino acids and few peptide chains; tryptone by contrast is the product of an incomplete enzymatic hydrolysis with some oligopeptides present.
Tryptone is also a component of some germination media used in plant propagation.[3]
^Fraser, Dean; Powell, Richard (1950). "The Kinetics of Trypsin Digestion" (PDF). The Journal of Biological Chemistry. 187 (2): 803–820. doi:10.1016/S0021-9258(18)56227-X. PMID 14803465. Retrieved 28 July 2014.
^"Indole Test". American Society for Microbiology. 8 Dec 2009. Archived from the original on 7 December 2013.
^Pierik, R. L. M., et al. (1988). Seed germination and further development of plantlets of Paphiopedilum ciliolare Pfitz. in vitro. Scientia Horticulturae, 34(1), 139-153.
Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. Tryptone is commonly used in microbiology to produce...
ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids such as peptides and peptones...
metallic green colonies on a dark purple medium. Also can be cultured on Tryptone Bile X-Glucuronide (TBX) to appear as blue or green colonies after incubation...
microbiologist may run a series of tests in the lab. These include: Phenol red Tryptone broth Phenylalanine agar for detection of production of deaminase, which...
list (link) Waterworth, Pamela M. (March 1972). "The lethal effect of tryptone-soya broth". Journal of Clinical Pathology. 25 (3): 227–228. doi:10.1136/jcp...
of hemolytic activity. BAPs also contain meat extract or yeast extract, tryptone, sodium chloride, and agar. Chocolate agar is a type of blood agar plate...
casein, a family of phosphoproteins found in mammalian milk. In comparison, tryptone describes casein that has undergone enzymatic degradation by the protease...
lactose to acid that causes a pH change in the medium. TYEA medium contains tryptone, yeast extract, common salt and L-arabinose per liter of glass distilled...
production with the addition of glucose, xylose and several carboxylic acids. Tryptone and various forms of yeast and yeast derivatives are traditionally used...
protein used in growth media to culture bacteria and other organisms, e.g. tryptone in Lysogeny Broth. Proteases may be classified according to the catalytic...
protein, such as soy meal; as pre-digested polypeptides, such as peptone or tryptone; or as ammonia or nitrate salts. Cost is also an important factor in the...
propagate. Germination media are most effective when they contain sugar and tryptone. Wikimedia Commons has media related to Paphiopedilum ciliolare. Wikispecies...
Monitoring methods will all use either a general purpose culture medium like tryptone soya agar (TSA), which will be used at a dual incubation regime of 30 °C...
and grows exclusively on complex substrates, such as peptone, casein, tryptone, and yeast extract. It cannot use carbon dioxide as a source of carbon...
blood medium, fastidious anaerobe broth, brain heart infusion medium, and tryptone yeast extract with glucose. Anaerobic bacteria like P. vulgatus are normally...
L1-L4, were acquired from the garden pond and successfully isolated in tryptone soy agar at room temperature (Frühling et al., 2002). E. undae is a Gram-positive...
Deinococcus aerius has been cultured on mTGE agar which contains beef extract, tryptone, glucose and Difto Bacto agar. A growth analysis revealed that this bacterium...
extract, maltose, cellobiose, β-glucans, starch, and protein sources (tryptone, peptone, casein, and meat extracts) through the Embden-Meyerhoff pathway...
the study done by Worley et al (1979), Fonticula alba was cultured on a tryptone-glucose-yeast-extract agar. Worley et al. (1979), also investigated the...
fungal strain of Aspergillus flavus growing in a surface culture on a tryptone-salt[clarification needed] was capable of producing a bactericidal filtrate...
were observed with complex organics such as yeast extract, meat extract, tryptone, and peptone as substrates) and inorganic compounds during aerobic and...
metabolism in the form of peptides (i.e. from yeast extract, peptone, or tryptone) and proteins (i.e. casein) as a carbon source which are oxidized to carbon...
proteinaceous, complex substrates such as yeast extract, peptone, and tryptone. Growth was not observed on single sugars or amino acids such as D-glucose...
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