Casamino acid is the mixture of amino acids produced from acid hydrolysis of casein, a family of phosphoproteins found in mammalian milk. In comparison, tryptone describes casein that has undergone enzymatic degradation by the protease trypsin, leaving many smaller peptide chains alongside the free amino acids.[1]
Casamino acid is used as a component of microbiological growth medium to support protein synthesis. However, tryptophan, an essential amino acid, is lost while digesting the casein with strong acids.[2] Providing this abundant source of nitrogen can alter the phenotype of microorganisms, such as bacterial species recognizing nutrient-rich media and reducing their motility, as compared to their typical environmental behaviors.[3]
^Klemann, S. W.; Li, J. Z.; Imakawa, K.; Cross, J. C.; Francis, H.; Roberts, R. M. (October 1990). "The Production, Purification, and Bioactivity of Recombinant Bovine Trophoblast Protein-1 (Bovine Trophoblast Interferon)". Molecular Endocrinology. 4 (10): 1506–1514. doi:10.1210/mend-4-10-1506. ISSN 0888-8809. PMID 2178217. S2CID 22254934.
^Nagappa, Lakshmeesha K.; Sato, Wakana; Alam, Farzana; Chengan, Kameshwari; Smales, Christopher M.; Von Der Haar, Tobias; Polizzi, Karen M.; Adamala, Katarzyna P.; Moore, Simon J. (2022). "A Ubiquitous Amino Acid Source for Prokaryotic and Eukaryotic Cell-Free Transcription-Translation Systems". Frontiers in Bioengineering and Biotechnology. 10: 992708. doi:10.3389/fbioe.2022.992708. ISSN 2296-4185. PMC 9524191. PMID 36185432.
^Samuels, Toby; Pybus, David; Cockell, Charles S. (25 November 2019). "Casamino Acids Slow Motility and Stimulate Surface Growth in an Extreme Oligotroph". Environmental Microbiology Reports. 12 (1): 63–69. doi:10.1111/1758-2229.12812. hdl:20.500.11820/4ab305fa-1989-4220-8453-d062af8e81f1. ISSN 1758-2229. PMID 31769203. S2CID 208298258.
Casaminoacid is the mixture of amino acids produced from acid hydrolysis of casein, a family of phosphoproteins found in mammalian milk. In comparison...
amino acids for the growing bacteria. Tryptone is similar to casaminoacids, both being digests of casein, but casaminoacids can be produced by acid hydrolysis...
pigment production can be restored in such contaminated isolates using casaminoacids erythritol albumin agar (CEA). T. rubrum cultures can be isolated on...
also displays this behaviour on vitamin-free, thiamine-supplemented casaminoacids agar but the growth is more sparse, and subsurface growth is absent...
contents such as KH2PO4, K2HPO4, NaCl, SO4, sodium citrate, yeast extract, Casaminoacids and trace elements. After inoculation of the sample, the enrichments...
from the C7 strain of Corynebacterium diphtheriae grown in a medium of casaminoacids and yeast extracts. Bacteria bearing the vaccine's polysaccharide sugars...
test results occur for the urease test, hair perforation test, and casaminoacids erythritol albumin agar. In conjunction to physiological tests, contact...
bacterium isolated from coastal marine sediment via enrichment with Casaminoacids". International Journal of Systematic and Evolutionary Microbiology...
their carbon source, but grow optimally when either yeast extract or CasaminoAcids are used. Currently, C. aggregans are known to reside in microbial mats...
temperature (23 °C) in a liquid media called R2B, containing peptone, casaminoacids, yeast extract, glucose, soluble starch, sodium pyruvate and inorganic...
carbon sources including d-sorbitol, glucose, malic acid, mannitol and sucrose and casaminoacids. Production of exopolysaccharide was hypothesized to...
methanol, formamide, formate, malate, propionate, lactate, acetate, and casaminoacids. The first of the Pyrobaculum species to be genetically sequenced, P...
with optimal growth at 7.25. Thermocrinis jamiesonii can use peptone, Casaminoacids, and acetate as carbon sources for chemolithoheterotrophic growth. It...
most substrates tested as a sole carbon source but could use peptone, casaminoacids or yeast extract as sole carbon sources. The strain did not degrade...
contents are extracted), casaminoacids (an amino acid/peptide mixture common to microbial growth media formed from the acid hydrolysis of casein), nor...
fructose, mannose, maltose, glycerol, oleate, stearate, palmitate and casaminoacids, whereas galactose, starch and glycogen are all poor sources of carbon...
and its highest rates of growth are seen when grown mixotropically on casaminoacids and metal sulfides. The dissimilatory oxidation of iron and sulfur in...
sugars as an energy source to stimulate growth. It can fix nitrogen from casaminoacids and reduces nitrates to nitrites. Cells are non-motile and occur in...