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Casamino acid information


Casamino acid is the mixture of amino acids produced from acid hydrolysis of casein, a family of phosphoproteins found in mammalian milk. In comparison, tryptone describes casein that has undergone enzymatic degradation by the protease trypsin, leaving many smaller peptide chains alongside the free amino acids.[1]

Casamino acid is used as a component of microbiological growth medium to support protein synthesis. However, tryptophan, an essential amino acid, is lost while digesting the casein with strong acids.[2] Providing this abundant source of nitrogen can alter the phenotype of microorganisms, such as bacterial species recognizing nutrient-rich media and reducing their motility, as compared to their typical environmental behaviors.[3]

  1. ^ Klemann, S. W.; Li, J. Z.; Imakawa, K.; Cross, J. C.; Francis, H.; Roberts, R. M. (October 1990). "The Production, Purification, and Bioactivity of Recombinant Bovine Trophoblast Protein-1 (Bovine Trophoblast Interferon)". Molecular Endocrinology. 4 (10): 1506–1514. doi:10.1210/mend-4-10-1506. ISSN 0888-8809. PMID 2178217. S2CID 22254934.
  2. ^ Nagappa, Lakshmeesha K.; Sato, Wakana; Alam, Farzana; Chengan, Kameshwari; Smales, Christopher M.; Von Der Haar, Tobias; Polizzi, Karen M.; Adamala, Katarzyna P.; Moore, Simon J. (2022). "A Ubiquitous Amino Acid Source for Prokaryotic and Eukaryotic Cell-Free Transcription-Translation Systems". Frontiers in Bioengineering and Biotechnology. 10: 992708. doi:10.3389/fbioe.2022.992708. ISSN 2296-4185. PMC 9524191. PMID 36185432.
  3. ^ Samuels, Toby; Pybus, David; Cockell, Charles S. (25 November 2019). "Casamino Acids Slow Motility and Stimulate Surface Growth in an Extreme Oligotroph". Environmental Microbiology Reports. 12 (1): 63–69. doi:10.1111/1758-2229.12812. hdl:20.500.11820/4ab305fa-1989-4220-8453-d062af8e81f1. ISSN 1758-2229. PMID 31769203. S2CID 208298258.

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Casamino acid

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Casamino acid is the mixture of amino acids produced from acid hydrolysis of casein, a family of phosphoproteins found in mammalian milk. In comparison...

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Tryptone

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amino acids for the growing bacteria. Tryptone is similar to casamino acids, both being digests of casein, but casamino acids can be produced by acid hydrolysis...

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R2A agar

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fuller, complex organic media.[citation needed] Proteose peptone, 0.05% Casamino acids, 0.05% Yeast extract, 0.05% Dextrose, 0.05% Soluble starch, 0.05% Dipotassium...

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Trichophyton rubrum

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pigment production can be restored in such contaminated isolates using casamino acids erythritol albumin agar (CEA). T. rubrum cultures can be isolated on...

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List of vaccine excipients

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Pneumococcal vaccine (Prevnar) aluminium phosphate, ammonium sulfate, casamino acid, polysorbate 80, succinate buffer, yeast Polio vaccine (IPV – Ipol)...

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Trichophyton tonsurans

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also displays this behaviour on vitamin-free, thiamine-supplemented casamino acids agar but the growth is more sparse, and subsurface growth is absent...

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Cloacibacillus evryensis

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contents such as KH2PO4, K2HPO4, NaCl, SO4, sodium citrate, yeast extract, Casamino acids and trace elements. After inoculation of the sample, the enrichments...

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Pneumococcal conjugate vaccine

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from the C7 strain of Corynebacterium diphtheriae grown in a medium of casamino acids and yeast extracts. Bacteria bearing the vaccine's polysaccharide sugars...

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Trichophyton verrucosum

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test results occur for the urease test, hair perforation test, and casamino acids erythritol albumin agar. In conjunction to physiological tests, contact...

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Halodesulfovibrio marinisediminis

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bacterium isolated from coastal marine sediment via enrichment with Casamino acids". International Journal of Systematic and Evolutionary Microbiology...

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Chloroflexus aggregans

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their carbon source, but grow optimally when either yeast extract or Casamino Acids are used. Currently, C. aggregans are known to reside in microbial mats...

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Terriglobus roseus

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temperature (23 °C) in a liquid media called R2B, containing peptone, casamino acids, yeast extract, glucose, soluble starch, sodium pyruvate and inorganic...

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Variovorax paradoxus

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carbon sources including d-sorbitol, glucose, malic acid, mannitol and sucrose and casamino acids. Production of exopolysaccharide was hypothesized to...

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Eleftheria terrae

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glucose, 1g yeast extract, 0.1g casamino acids, 3g proline, 10g MgCl2·6H2O, 4g CaCl2·2H2O, 0.2g K2SO4, 5.6g TES free acid per liter of deionized H2O at...

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Pyrobaculum

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methanol, formamide, formate, malate, propionate, lactate, acetate, and casamino acids. The first of the Pyrobaculum species to be genetically sequenced, P...

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Thermocrinis jamiesonii

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with optimal growth at 7.25. Thermocrinis jamiesonii can use peptone, Casamino acids, and acetate as carbon sources for chemolithoheterotrophic growth. It...

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Fimbriimonas ginsengisoli

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most substrates tested as a sole carbon source but could use peptone, casamino acids or yeast extract as sole carbon sources. The strain did not degrade...

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Acidithiobacillus caldus

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contents are extracted), casamino acids (an amino acid/peptide mixture common to microbial growth media formed from the acid hydrolysis of casein), nor...

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Conidiobolus coronatus

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fructose, mannose, maltose, glycerol, oleate, stearate, palmitate and casamino acids, whereas galactose, starch and glycogen are all poor sources of carbon...

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Vulcanisaeta

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utilizes maltose, starch, malate, yeast extract, peptone, beef extract, casamino acids and gelatin as carbon sources, cannot utilize D-arabinose, D-fructose...

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Metallosphaera sedula

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and its highest rates of growth are seen when grown mixotropically on casamino acids and metal sulfides. The dissimilatory oxidation of iron and sulfur in...

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Natronococcus

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sugars as an energy source to stimulate growth. It can fix nitrogen from casamino acids and reduces nitrates to nitrites. Cells are non-motile and occur in...

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