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Total internal reflection microscopy information


Scattering of an evanescent field by a probe particle.

Total internal reflection microscopy is a specialized optical imaging technique for object tracking and detection utilizing the light scattered from an evanescent field in the vicinity of a dielectric interface. Its advantages are a high signal-to-noise ratio and a high spatial resolution in the vertical dimension.

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Total internal reflection microscopy

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Total internal reflection microscopy is a specialized optical imaging technique for object tracking and detection utilizing the light scattered from an...

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Total internal reflection

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In physics, total internal reflection (TIR) is the phenomenon in which waves arriving at the interface (boundary) from one medium to another (e.g., from...

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Total internal reflection fluorescence microscope

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A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers...

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Microscopy

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microscopy.[citation needed] Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron...

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Evanescent field

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biological cells or single protein and DNA molecules for microscopy (as in the total internal reflection fluorescence microscope). The evanescent wave from...

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Fluorescence microscope

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more complex fluorescence microscopy techniques like confocal microscopy and total internal reflection fluorescence microscopy while xenon lamps, and mercury...

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Colloidal probe technique

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the surface forces apparatus, total internal reflection microscopy, and optical tweezers techniques to with video microscopy. The possibility to measure...

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Optical sectioning

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only interact with the surface of the sample. Total internal reflection microscopy is a fluorescent microscopy technique, which intentionally restricts observation...

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Confocal microscopy

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microscope Super-resolution microscopy Total internal reflection fluorescence microscope (TIRF) Two-photon excitation microscopy: Although they use a related...

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List of materials analysis methods

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backscattering spectrometry REM – Reflection electron microscopy RDS – Reflectance difference spectroscopy RHEED – Reflection high energy electron diffraction...

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Photoactivated localization microscopy

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Photo-activated localization microscopy (PALM or FPALM) and stochastic optical reconstruction microscopy (STORM) are widefield (as opposed to point scanning...

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Numerical aperture

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{n_{\text{clad}}}{n_{\text{core}}}}} is the critical angle for total internal reflection. Substituting cos θc for sin θr in Snell's law we get: n n core...

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Supercritical angle fluorescence microscopy

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Seeger: Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy, Optic Letters 29, 569-571 (2004) Hill, D; McDonnell...

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Photon scanning microscopy

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induce total internal reflection within the prism. Although the beam of light is not propagated through the surface of the refractive prism under total internal...

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Nicol prism

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It is made in such a way that it eliminates one of the rays by total internal reflection, i.e. the ordinary ray is eliminated and only the extraordinary...

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Refractive index

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when reaching the interface, as well as the critical angle for total internal reflection, their intensity (Fresnel equations) and Brewster's angle. The...

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Uppendahl prism

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Uppendahl. The other reflections of the beam take place by means of loss-free total internal reflection. The second prism is a 90° reflection and shouldn't need...

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Scanning acoustic microscope

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penetration depth of acoustic waves to image the internal structure of the specimen. So, in scanning acoustic microscopy either reflected or transmitted acoustic...

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Fluorescence imaging

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fluorescence to observe a biological process. Total internal reflection fluorescence microscopy: A microscopy technique that uses evanescent waves to selectively...

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Wellcome Centre for Human Genetics

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Lifetime Imaging Microscopy (FLIM), Total Internal Reflection Fluorescence Microscopy (TIRF), Photoactivated Localisation Microscopy (PALM), Spectral...

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Vertico spatially modulated illumination

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limit (about 200 nm for blue light) applying to standard microscopy using transmission or reflection of natural light (as opposed to structured illumination...

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Elastic scattering

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Scattering theory Thomson scattering “Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for materials characterization,” B.J. Inkson...

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Calcium concentration microdomains

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concentration microdomains and vesicle fusion by using total internal reflection fluorescent microscopy". Proceedings of the National Academy of Sciences....

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Andor Technology

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images Total internal reflection fluorescence (TIRF) – for studying cellular membranes and their associated molecules Multi-photon microscopy systems...

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Centre for Genomic Regulation

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microscopy, two photon microscopy, confocal microscopy, total internal reflection fluorescence microscopy, fluorescence lifetime imaging microscopy,...

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Vibrational analysis with scanning probe microscopy

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Mizaikoff, Boris (2007). "Combined in Situ Atomic Force Microscopy- Infrared-Attenuated Total Reflection Spectroscopy". Analytical Chemistry. 79 (22): 8803–8806...

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