Total internal reflection fluorescence microscope information
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed.
TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide. The technique is based on the principle that when excitation light is totally internally reflected in a transparent solid coverglass at its interface with a liquid medium, an electromagnetic field, also known as an evanescent wave, is generated at the solid-liquid interface with the same frequency as the excitation light.[1] The intensity of the evanescent wave exponentially decays with distance from the surface of the solid so that only fluorescent molecules within a few hundred nanometers of the solid are efficiently excited. Two-dimensional images of the fluorescence can then be obtained, although there are also mechanisms in which three-dimensional information on the location of vesicles or structures in cells can be obtained.[2]
^Fish, Kenneth N. (October 2009). "Total Internal Reflection Fluorescence (TIRF) Microscopy". Current Protocols in Cytometry. 0 12: Unit12.18. doi:10.1002/0471142956.cy1218s50. ISSN 1934-9297. PMC 4540339. PMID 19816922.
A totalinternalreflectionfluorescencemicroscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers...
A fluorescencemicroscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption...
protein and DNA molecules for microscopy (as in the totalinternalreflectionfluorescencemicroscope). The evanescent wave from an optical fiber can be...
limitations of traditional wide-field fluorescencemicroscopes. In a conventional (i.e., wide-field) fluorescencemicroscope, the entire specimen is flooded...
TIRF may refer to: Totalinternalreflectionfluorescencemicroscope Traffic Injury Research Foundation, in partnership with Toyota Canada Inc. Trans-inclusionary...
and phase-separated polymers and RNA/protein condensates. Totalinternalreflectionfluorescence (TIRF) is a microscopy approach that is only sensitive to...
all utilize the same mechanism of fluorescence to observe a biological process. Totalinternalreflectionfluorescence microscopy: A microscopy technique...
Fluorescence is one of two kinds of emission of light by a substance that has absorbed light or other electromagnetic radiation. Fluorescence involves...
anaesthetic action Thomas A. Steitz Thomas Gold Thylakoid Totalinternalreflectionfluorescencemicroscope Tradeoffs for locomotion in air and water Transepithelial...
simulations. She uses a confocal super-resolution microscope and Totalinternalreflectionfluorescencemicroscope to image dynamic events over long periods....
blood smear review, in which the blood is stained and viewed under a microscope to verify that the analyzer results are consistent with the appearance...
these microscopes only interact with the surface of the sample. Totalinternalreflection microscopy is a fluorescent microscopy technique, which intentionally...
conventional fluorescence-activated cell sorting. An evanescent field is a residue optical field that "leaks" during totalinternalreflection. This "leaking"...
induce totalinternalreflection within the prism. Although the beam of light is not propagated through the surface of the refractive prism under total internal...
sciences. Spinning disk confocal microscopes – for capturing high-speed live cell images Totalinternalreflectionfluorescence (TIRF) – for studying cellular...
may be lost via luminescence, fluorescence and phosphorescence. A molecule absorbs the photon which results in reflection or scattering. A molecule cannot...
"Core–shell silica–rhodamine B nanosphere for synthetic opals: from fluorescence spectral redistribution to sensing". RSC Advances. 10 (25): 14958–14964...
microscopy using transmission or reflection of natural light (as opposed to structured illumination or fluorescence) according to the Abbe resolution...
with a refractive index of about 3.5, escape is inhibited by totalinternalreflection and (2) experiments at 90K report narrow-beam infrared absorption...
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