TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA.
In molecular biology, it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA.[1] It is made up of Tris-acetate buffer, usually at pH 8.3, and EDTA, which sequesters divalent cations. TAE has a lower buffer capacity than TBE and can easily become exhausted, but linear, double stranded DNA runs faster in TAE.
Previously, Brody & Kern simplified electrophoretic buffers by substituting TBE and TAE buffers for a more efficient and inexpensive conductive media in gel systems.[2]
^Ogden, R.C., and Adams, D.A., (1987) Electrophoresis in agarose and acrylamide gels. Methods Enzymol., 152:, 61-87.
^Brody, J.R., Kern, S.E. (2004) History and principles of conductive media for standard DNA electrophoresis. Anal Biochem. 333(1):1-13. doi:10.1016/j.ab.2004.05.054 PMID 15351274 PDF
TAEbuffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. In molecular biology, it is used in agarose electrophoresis typically...
Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA. In molecular biology, TBE and TAEbuffers are often used in...
of Tris TAEbuffer and TBE buffer are often used in procedures involving nucleic acids, the most common being electrophoresis. ph7.4 TE buffer=100mM/L...
that operated from 1967 to 1981 TAEbuffer, a solution used in chemistry TAE connector, for German telephone equipment Tae' language, an Austronesian language...
biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids. It contains...
heated. However, SB buffer has lower conductivity than TBE and TAE, and thus the gel temperature is much lower than with TBE or TAEbuffers. Therefore, the...
varies according to gel density and buffer composition, but in a typical 1% agarose gel in a 1X TAEbuffer or TBE buffer, bromophenol blue migrates at the...
Lithium Borate buffer has a lower conductivity, produces crisper resolution, and can be run at higher speeds than can gels made from TBE or TAE (5-50 V/cm...
as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer...
with the concentration of agarose and the buffer system used. For instance, in a 1% agarose gel made in TAEbuffer (Tris-acetate-EDTA), xylene cyanol migrates...
powder in an appropriate buffer, such as TAE or TBE, to be used in electrophoresis. The agarose is dispersed in the buffer before heating it to near-boiling...
divides the peninsula roughly in half. It was established to serve as a buffer zone between the countries of North Korea and South Korea under the provisions...
of buffers used for electrophoresis. The most common being, for nucleic acids Tris/Acetate/EDTA (TAE), Tris/Borate/EDTA (TBE). Many other buffers have...
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coming at "around the same time." The shells fell into the ocean in the buffer zone between the two countries. No damage was reported. "Inter-Korean MDL"...
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carbonic acid, and the bicarbonate buffer system can also come into play. Renal compensation can help the bicarbonate buffer system. The sensor for the plasma...
President Yeltsin handed the two recorder containers to Korean President Roh Tae-Woo, but not the tapes themselves. The following month, the ICAO voted to...
the bicarbonate buffer system (HCO3-/CO2), which allows maintaining a constant pH level of the blood and extracellular fluid. This buffer system is described...
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